Immunogen was polypeptide PKCG (305-308aa)-KLH:CNYPLELYERVRMG. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with the immunogen. The IgG fraction of tissue culture supernatant was purified by Protein G/A affinity chromatography.
Immunogen Sequence
KLH:CNYPLELYERVRMG
Recommended Applications
ELISA (recommended work dilution = 1:80,000)
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in 1 x PBS. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
Estimated Lead Time
1-2 business days
Shipping Type
Ambient temperature
Storage
-20°C
Description
The gamma isotype of protein kinase C (PKC gamma) is a member of the classical PKC (cPKC) subfamily which is activated by Ca(2+) and diacylglycerol in the presence of phosphatidylserine. Physiologically, PKC gamma is activated by a mechanism coupled with receptor-mediated breakdown of inositol phospholipid as other cPKC isotypes such as PKC alpha and PKC beta. PKC gamma is expressed solely in the brain and spinal cord and its localization is restricted to neurons, while PKC alpha and PKC beta are expressed in many tissues in addition to the brain.
Storage
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.
