Mouse Activin A ELISA Kit

Name: Mouse Activin A ELISA Kit
Brand: ELM-ActivinA
SKU: ELM-ActivinA
Price: 420 USD
Availability: InStock

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RayBio® Mouse Activin A ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

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Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Mouse
Accession Number	Q04998
Gene Id	16323
Protein Name / Synonyms	Inhibin beta A chain (Activin beta-A chain)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA antibody pair detects Mouse, Human and Rat Activin A beta A subunit. Other species not determined. This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse 6Ckine (CCL21), ADAMTS-1 (METH1), Adiponectin (ACRP30), Angiopoietin-3 (ANGPTL1), ANGPTL3, Artemin, CCL28 (MEC), CD36 (SR-B3), Chordin, CRP (C-Reactive Protein), E-Cadherin, Epigen, Epiregulin, Fas (TNFRSF6/Apo-1), Galectin-7, gp130, Granzyme B, Gremlin-1, IFN-gamma R1, IL-17 RB, IL-17B, IL-22, MIP-1 beta (CCL4), MMP-10, MMP-2, MMP-3, PDGF-AA, Persephin, sFRP-3, SLAM (CD150), Sonic Hedgehog NTerminal (Shh-N), TCK-1 (CXCL7), TECK (CCL25), TGF beta 1, Trance (TNFSF11), TREML1 (TLT-1), TWEAK (TNFSF12), VEGF-B, VEGFR2.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	0.5 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	0.5 pg/ml - 100 pg/ml
Recommended Dilution (Serum/Plasma)	15 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELM-ActivinA.pdf)

SDS (ELM-ActivinA.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Schumann C., Nguyen D., Norgard M., et al. Increasing lean muscle mass in mice via nanoparticle-mediated hepatic delivery of follistatin mRNA. Theranostics. 2018; 8(19): 5276-5288. Published online 2018 Oct 22. doi: 10.7150/thno.27847

Species:

Mouse

Sample Type:

Serum (Nanoparticle testing)

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Mouse Activin A ELISA Kit