The RayBiotech Mpox (formerly known as monkeypox) Virus 1-Step High Throughput PCR Kit is a ready to use PCR assay for the detection of Mpox virus (MPXV) DNA. The 1-Step Nucleic Acid Stabilization Buffer eliminates the need for DNA extraction and purification steps.
No cross reactivity was identified when the kit was evaluated against other common Orthopox family members: Cowpox virus, Camelpox virus, and Vaccinia virus.
Mpox virus is a virus from the orthopox family of viruses, a family most often associated with the more severe smallpox virus (variola), or the origination of vaccinations from the cowpox virus. Originally named for an outbreak among monkeys, it was first identified in humans in central and western Africa around 1970 where it continues to circulate at a low endemic level. While the virus is clinically less severe than smallpox, the symptoms are similar. With vaccinations against smallpox ceasing in 1972 and the eradication of the smallpox virus, vaccinations in the younger population (<50 years of age) is extremely limited. The escape from the endemic regions has the World Health Organization on alert given the lack of associated travel within the region of those suspected and confirmed cases. Understanding how the virus is being spread, the populations it is spreading to, and the potential immune responses against the virus are of increasing importance in the research field.
The RayBiotech Mpox Virus 1-Step High Throughput PCR Kit is a ready to use PCR assay for the detection of MPXV DNA. It uses a fluorescent probe with specific primer sets to detect the J2R gene specific for the genome of MPXV. Primer and probe for an internal control, RNase P are also integrated in the assay to validate the assay quality. The kit also contains a proprietary buffer which enables the user to use crude skin swabs directly in their PCR reactions. By simply mixing the 1-Step Nucleic Acid Stabilization Buffer with the sample, the viral DNA is immediately released and stabilized, and interfering components of the human tissue are inactivated. This eliminates the need for laborious, costly DNA extraction and purification steps, drastically improving upon the traditional workflow of PCR, while removing potential sample loss and contamination.
Product Features
Mpox virus specific / no cross-reacitvity with other Orthopox viruses.
Hot-start Taq DNA Polymerase with high efficiency and fidelity.
UDG and dUTP system minimizes carry-over contamination.
ROX reference dye improves accuracy and reproducibility.
Kit Components
Component
Ingredients
Specification
Quantity
2x PCR Reaction Solution
Buffer, dNTP’s, enzyme, ROX reference dye
1000 µL / tube
1 tube
Primers and Probe Mix
MPXV/RNase P Primer & Probe Mix
500 μl / tube
1 tube
Positive Control
Synthetic MPXV genomic DNA/RNase P Positive Control
50 µL / tube
1 tube
Negative Control
Nuclease-free water
500 µL / tube
1 tube
1-Step Nucleic Acid Stabilization Buffer
Buffer
10 mL / bottle
1 bottle
Other Materials Required
VTN DNA preservation solution
Fluorescence PCR instrument capable of reading FAM or equivalent channel (494 nm maximum absorption, 518 nm maximum emission) and TAMRA or equivalent channel (550 nm maximum absorption, 518 nm maximum emission).
Vortex Mixer
Microcentrifuge
Pipettes
Sterile nuclease-free pipette tips (barrier tips recommended) and microfuge tubes
The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C.
