Lipid Peroxidation (MDA) Assay Kit
The RayBiotech Lipid Peroxidation (MDA) Assay Kit provides a simple, reproducible, and standardized tool for assaying lipid peroxidation in serum, plasma, cell or tissue lysates, urine, or other body fluid samples.
Product Description
Specifications
| Size | 2 Plate Kit |
|---|---|
| Quantitative/Semi-Quantitative | Quantitative |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Research Area | Cardiovascular Disease, Inflammation, Oxidation |
| Sensitivity | The minimum detectable concentration of MDA is 0.38 uM. |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | 2-8°C |
| Storage / Stability | The entire kit may be stored at 2-8 °C for up to 6 months from the date of shipment. For prepared reagent storage, see manual. |
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| Component | Size / Description |
|---|---|
| Microplates | Two 96-well plates (12 strips x 8 wells) |
| MDA Standard | 1 vial (25 µl) |
| Thiobarbituric Acid (TBA) | 1 bottle (1g white powder) |
| SDS Solution | 20 ml |
| 2X TBA Acid Diluent | 25 ml |
| Sodium Hydroxide Solution | 5 ml |
| 100X BHT Solution | 1 vial (1 ml) |
Additional Materials Required
- 1.5 ml microcentrifuge tubes and 50 ml conical tubes
- Benchtop centrifuge and microcentrifuge
- Water bath or heat block capable of 95°C
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- Spectrophotometric microplate reader capable of reading at 532 nm
- Analytical balance
Assay Procedure Summary
Prepare and mix all reagents thoroughly before use. Each MDA-containing sample and standard should be assayed in duplicate. High content MDA samples can be further diluted for analysis.
- Pipette 100 µl of samples or prepared MDA series standards (see Pre-Assay Preparation, B) to each microcentrifuge tube.
- Add 100 µl of the SDS Solution to each sample and the MDA standards. Mix thoroughly and quick spin, Incubate samples for 5 minutes at room temperature.
- Add 250 µl of prepared TBA Reagent (see Pre-Assay Preparation, A-3) to each sample and standard. Mix thoroughly then incubate at 95°C for 60 minutes.
- Put all tubes on ice and incubate for 5 minutes.
- Centrifuge all tubes at 1600 x g for 10 minutes.
- Transfer 200 µL of supernatant from each sample and the MDA standards to a 96 well microplate. Read the absorbance at 532 nm immediately.
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Good and easy to use kitThis kit is really worked with urine and bladder tissue samples. The protocol is very straight forward.
from Weill Cornell Medicine, New York,
on
Easy to useThis Kit is really easy to use and works efficiently in both cell lysates and tissue lysates. The protocol description is apparent and it works well.from Oncology Science Department, OUHSC, OKC, OK,
on
Good productWorking with both tissue lysate and effluent. Protocol is very straightforward. Only time consuming portion is that samples need to be prepared in microcentrifuge tubes instead of a 96-well plate as it has a 90ºC incubation, so it is not time-effective if you plan to run a lot of samples. Otherwise it's a good assay!from N/A,
on
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