Insulin Epitope Mapping Peptide Array
The Insulin Epitope Mapping Peptide Array is a semi-quantitative array displaying 75 synthesized peptides representing the full-length Insulin. It is suitable for antibody epitope mapping and detection of IgG, IgA, IgM, IgE, etc. in serum, plasma, and many other liquid sample types.
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Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit, 16 Sample Kit |
|---|---|
| Species | Human |
| Accession Number | P01308 |
| Protein Name / Synonyms | Insulin [Cleaved into: Insulin B chain, Insulin A chain] |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 75 |
| Compatible Sample Types | Plasma, Serum, Ascites, Hybridoma cell culture medium, Purified antibodies, Purified protein, DNA Aptamers, Other fluids |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Antibody Drug Development, Drug Antibody (DA) Detection |
| Estimated Lead Time | 4-6 weeks |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| 1. FVNQHLCGSHLV | 2. VNQHLCGSHLVE | 3. NQHLCGSHLVEA | 4. QHLCGSHLVEAL |
| 5. HLCGSHLVEALY | 6. LCGSHLVEALYL | 7. CGSHLVEALYLV | 8. GSHLVEALYLVC |
| 9. SHLVEALYLVCG | 10. HLVEALYLVCGE | 11. LVEALYLVCGER | 12. VEALYLVCGERG |
| 13. EALYLVCGERGF | 14. ALYLVCGERGFF | 15. LYLVCGERGFFY | 16. YLVCGERGFFYT |
| 17. LVCGERGFFYTP | 18. VCGERGFFYTPK | 19. CGERGFFYTPKT | 20. GERGFFYTPKTR |
| 21. ERGFFYTPKTRR | 22. RGFFYTPKTRRE | 23. GFFYTPKTRREA | 24. FFYTPKTRREAE |
| 25. FYTPKTRREAED | 26. YTPKTRREAEDL | 27. TPKTRREAEDLQ | 28. PKTRREAEDLQV |
| 29. KTRREAEDLQVG | 30. TRREAEDLQVGQ | 31. RREAEDLQVGQV | 32. REAEDLQVGQVE |
| 33. EAEDLQVGQVEL | 34. AEDLQVGQVELG | 35. EDLQVGQVELGG | 36. DLQVGQVELGGG |
| 37. LQVGQVELGGGP | 38. QVGQVELGGGPG | 39. VGQVELGGGPGA | 40. GQVELGGGPGAG |
| 41. QVELGGGPGAGS | 42. VELGGGPGAGSL | 43. ELGGGPGAGSLQ | 44. LGGGPGAGSLQP |
| 45. GGGPGAGSLQPL | 46. GGPGAGSLQPLA | 47. GPGAGSLQPLAL | 48. PGAGSLQPLALE |
| 49. GAGSLQPLALEG | 50. AGSLQPLALEGS | 51. GSLQPLALEGSL | 52. SLQPLALEGSLQ |
| 53. LQPLALEGSLQK | 54. QPLALEGSLQKR | 55. PLALEGSLQKRG | 56. LALEGSLQKRGI |
| 57. ALEGSLQKRGIV | 58. LEGSLQKRGIVE | 59. EGSLQKRGIVEQ | 60. GSLQKRGIVEQC |
| 61. SLQKRGIVEQCC | 62. LQKRGIVEQCCT | 63. QKRGIVEQCCTS | 64. KRGIVEQCCTSI |
| 65. RGIVEQCCTSIC | 66. GIVEQCCTSICS | 67. IVEQCCTSICSL | 68. VEQCCTSICSLY |
| 69. EQCCTSICSLYQ | 70. QCCTSICSLYQL | 71. CCTSICSLYQLE | 72. CTSICSLYQLEN |
| 73. TSICSLYQLENY | 74. SICSLYQLENYC | 75. ICSLYQLENYCN | Â |
Application Notes
- Identify high antigenicity epitopes for antibody production & vaccine development
- Locate (auto)antigen epitopes of (auto)antibodies
- Define the best antibody clone against the protein-of-interest
- Perform epitope mutant analysis
- Map B cell epitopes
Service Features
- Small serum sample volume required (2 µl)
- High density (simultaneously analyze hundreds of overlapping peptides)
- High sensitivity
- Large dynamic range
- Suitable for high-throughput assays
- High efficiency and accuracy
- Affordable, quick and simple to use
Kit Components
- RayBio® human Insulin protein peptide array
- Blocking Buffer
- Wash Buffer I
- Wash Buffer II
- Biotin-conjugated secondary antibody
- Cy3 equivalent dye conjugated streptavidin
- Array accessories (including slide incubation chamber assembly, gasket, adhesive plastic strips, etc.)
Other Materials Required
- Distilled water
- Small plastic boxes or containers
- Orbital shaker or oscillating rocker
- Pipettors, pipette tips and other common lab consumables
- Aluminum foil
- Laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Storage/Stability
Upon receipt, all kit components should be stored at -20 °C to -80 °C until ready to use. Stored properly, the kit will retain complete activity for up to 6 months.
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