RayBio® Human VEGF-A ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
---|---|
Species | Human |
Accession Number | P15692 |
Gene Id | 7422 |
Gene Symbols | VEGFA|VEGF |
Protein Name / Synonyms | Vascular endothelial growth factor A (VEGF-A) (Vascular permeability factor) (VPF) |
Quantitative/Semi-Quantitative | Quantitative |
Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO. |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Solid Support | 96-well Microplate |
Method Of Detection | Colorimetric |
Design Principle | Sandwich-based |
Research Area | Angiogenesis, Antibody Drug Development, Protein Therapeutics, Biomarker Detection |
Sensitivity | 10 pg/ml Need more sensitivity? Check out the new BIQ-ELISAâ„¢ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
Detection Range | 10 pg/ml - 6000 pg/ml |
Recommended Dilution (Serum/Plasma) | 2 - 5 fold |
Estimated Lead Time | 1-2 business days |
Shipping Type | Blue ice |
Storage | ≤-20°C |
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Mause S., Ritzel E., Liehn E., et al. Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury. Circulation . 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473.
De Barros, Sandra, et al. "Aging-related decrease of human ASC angiogenic potential is reversed by hypoxia preconditioning through ROS production." Molecular Therapy 21.2 (2013): 399-408.
Fermoselle C., Rabinovich R., Ausin P., et al. Does oxidative stress modulate limb muscle atrophy in severe COPD patients? Eur Respir J. 2012 Oct;40(4):851-62.
Bigalke C., et al. VEGF-releasing suture material for enhancement of vascularization: 4 Development, in vitro and in vivo study. Acta Biomaterialia 7 September 2014, DOI: 10.1016/j.actbio.2014.09.002
Diaz-Gomez L., Concheiro A., Alvarez-Lorenzo C., Garcia-Gonzalez C. Growth factors delivery from hybrid PCL-starch scaffolds processed using supercritical fluid technology. Carbohydr Polym. 2016 May 20;142:282-92. doi: 10.1016/j.carbpol.2016.01.051.
Paget, M. B., et al. "Rotational co?culture of clonal ??cells with endothelial cells: effect of PPAR?? agonism in vitro on insulin and VEGF secretion." Diabetes, Obesity and Metabolism 13.7 (2011): 662-668.
Wang XS, Williams LA, Krishnan S, et al. Serum sTNFR1, IL6, and the Development of Fatigue in Patients with Gastrointestinal Cancer Undergoing Chemoradiation Therapy. Brain, Behavior, and Immunity. 2012;26(5):699-705. doi:10.1016/j.bbi.2011.12.007.
Wang, Y., Lan, H., Yin, T., Zhang, X., Huang, J., Fu, H., Wang, Z. (2019). Covalent immobilization of biomolecules on stent materials through mussel adhesive protein coating to form biofunctional films. Materials Science and Engineering: C, 110187. doi:10.1016/j.msec.2019.110187Â
Platt, DH et al. Peroxynitrite increases VEGF expression in vascular endothelial cells via STAT3. Peroxynitrite increases VEGF expression in vascular endothelial cells via STAT3. Peroxynitrite increases VEGF expression in vascular endothelial cells via STAT3. Free Radic. Biol. Med. Volume 39, Issue 10, Pages 1353-61, Nov 2005
Etulain, J., et al. "Hyperthermia inhibits platelet hemostatic functions and selectively regulates the release of alpha?granule proteins." Journal of Thrombosis and Haemostasis 9.8 (2011): 1562-1571.
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Easy to useWe have been using this kit for many years. Very easy to use.
from Medical University of SC,
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Great productVery easy to use. Excellent results.from Michigan State University,
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Great productIt is a very user-friendly product for people who work with this type of sample for the first time, highly recommended.from University of Puerto Rico,
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Great productThis kit is simple to use and ideal for those who are new to evaluating factors on culture media. All directions are simple to follow, and the reagents are high quality.from University of Puerto Rico at Mayaguez,
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Great productVery good sensitivityfrom BCM,
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Great productHad great success using these kits. Really clear results and effective. These kits are easy to use and come with all the components needed which is a plus! Look forward to using them again.from Advirna,
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Great productI've used this product several times and am very happy with its high sensitivity, ease of use, and the technical support.from State University of New York,
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Great productI used cell culture supernatant and it was really easy to performfrom CPMC,
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Great productUse it to test in vitro releasing of VEGF-A from tissue engineering scaffold. Easy to use and work very well.from North Carolina State University,
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Great productI used this kit for first time and works really goodfrom CPMC,
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Great productVery sensitive kit, instructions are easy to follow and have yielded great results!from University of Illinois,
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Great productWriting another review because we have had nothing but success with this kit! Looking to publish the results soon!from University of Florida,
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Great productEasy to use!from University of San Diego,
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Great productThe ELISA instructions are concise and easy to follow. The kit also has all reagents you need. It has great reproducibility and reliability.from University of Florida,
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Great productThis product has been reliable with a descriptive protocol booklet that is easy to follow.from Immutrix Therapeutics,
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Great productVery simple, concise instructions. This ELISA kit has a wide concentration detection range. It performed as intended and will continue using their ELISA kits. I fully recommend RayBiotech to anyone in lab.from Stanford University,
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Great productI'm really impressed by all Raybiotech ELISA kits. This one is not an exception: it works well with tumor tissue lysates, and allows to analyze tumor cell-derived VEGF-A in xenograft mouse models.from Roswell Park Comprehensive Cancer Center,
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Great productWe ordered 2 boxes of this kit (along side with 2 of Periostin and 2 of IL-4 ELISA kits). The kits came in strong, sturdy boxes. The instructions are clear and easy to follow. The only thing is we wish that the sensitivity was lower. The current minimum detectable dose of Human VEGFA is 10 pg/ml. We had to dilute the standards and remove one of the them from the standard curve to be able to detect the protein levels. But we used these kits on urine samples and it seemed like VEGFA was very low in urine anyways. Just an idea for future improvement.from New York Medical College,
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