Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit, 10 Plate Kit |
|---|---|
| Species | Human |
| Accession Number | P01137 |
| Gene Id | 7040 |
| Gene Symbols | TGFB1|TGFB |
| Protein Name / Synonyms | Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)] |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1alpha, TIMP-1, TIMP-2, TNF-alha, TGF-beta 2, TGF-beta 3, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Design Principle | Sandwich-based |
| Sensitivity | 18 pg/ml Need more sensitivity? Check out the new BIQ-ELISAâ„¢ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 18 pg/ml - 4000 pg/ml |
| Recommended Dilution (Serum/Plasma) | 20 fold after treatment (see activation steps on page 6) |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Very good and UserfriendlyThe quality of kit is very good. I have used it for culture supernatant. Company also provides excel sheet for calculation.
from Meharry medical College,
on
ELISA from cell culture supernatantI used it for characterizing monocytes derived macrophages M1 vs M2 from human monocytes as well as cell line. Works great, only thing is we need to activate TGF-B by heat or acidfrom Brigham and Women's Hospital,
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Great productUsed it to analyze hTGFB1 concentration in cells. Very easy to use and worked well.from Medical University of South Carolina,
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Great productUsed it for cell culture media from prostate epithelial cells and fibroblasts as part of a cytokine panel with other kits. Of note, this uses a different assay diluent (E2) than other ELISA kits in step 5 and 7. Assay worked well.from University of Arizona,
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Great productI used this ELISA to look for TGFbeta in media collected after LPS stimulation of THP-1 cells. Very easy to use kit. The only complaint is that solutions necessary for TGFb activation are not included, and not listed in the manual (in the section additional materials). Only midway through the sample preparation, there is a description of activation step.from Duke University,
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Great productCorrelates with Kiloplex resultsfrom University of Houston,
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Great productUseful in measuring hTGFB1 concentration for nanoparticle incorporation of growth factors. ELISA kit instructions were easy to follow and had a high sensitivity which is necessary in specific essays like this.from University of Wisconsin-Madison,
on
Great productI used it for human corneal epithelial cell culture supernatant under induced conditions and tested various treatments. It is very important to ensure that it's very high sensitivity and this product was very useful.from University of Illinois at Chicago,
on
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