Human RTK Phosphorylation Array G1
RayBio® G-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 71 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Research Area | Post-Translational Modifications, Phosphorylation, PI3K-AKT Signaling |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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$5 Amazon gift card in every kit box purchased.
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
ABL1
|
ACK
|
ALK-1
|
Axl
|
Blk
|
|
BMX
|
Btk
|
Csk
|
Dtk
|
EGFR
|
|
EphA1
|
EphA2
|
EphA3
|
EphA4
|
EphA5
|
|
EphA6
|
EphA7
|
EphA8
|
EphB1
|
EphB2
|
|
EphB3
|
EphB4
|
EphB6
|
ErbB2
|
ErbB3
|
|
ErbB4
|
FAK
|
FER
|
FGFR1
|
FGFR2
|
|
FGFR2 alpha isoform
|
Fgr
|
FRK
|
Fyn
|
Hck
|
|
HGFR
|
IGF-1 R
|
Insulin R
(CD220)
|
Itk
|
JAK1
|
|
JAK2
|
JAK3
|
LCK
|
LTK
|
Lyn
|
|
MATK
|
M-CSF R
|
MUSK
|
NGFR
(TNFRSF16)
|
PDGFRA
|
|
PDGFRB
|
PYK2
|
RET
|
ROR1
|
ROR2
|
|
ROS
|
RYK
|
SCF R
(CD117/c-kit)
|
SRMS
|
SYK
|
|
Tec
|
Tie-1
|
Tie-2
|
TNK1
|
TRKB
|
|
TXK
|
Tyk2
|
TYRO10
(DDR2/TKT)
|
VEGFR2
|
VEGFR3
|
|
ZAP70
|
||||
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human RTK Phosphorylation Antibody Array G1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail II
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Amit, Moran, and Ziv Gil. "Macrophages increase the resistance of pancreatic adenocarcinoma cells to gemcitabine by upregulating cytidine deaminase." Oncoimmunology 2.12 (2013): e27231.
Species:
Human
Sample Type:
Cell Lysate (PANC-1 pancreatic cancer cell line)
Zhang H., Pelech S. Using protein microarrays to study phosphorylation-mediated signal transduction. Semin Cell Dev Biol. 2012 Oct;23(8):872-82. doi: 10.1016/j.semcdb.2012.05.009
Species:
Human
Sample Type:
N/A (Review reference, no sample type, just a reference to our arrays)
Shabtay-Orbach A., Amit M., Binenbaum Y., et al. Paracrine regulation of glioma cells invasion by astrocytes is mediated by glial-derived neurotrophic factor. Int J Cancer. 2015 Sep 1;137(5):1012-20. doi: 10.1002/ijc.29380.
Species:
Human
Sample Type:
Cell Lysate (Human Glioma cell lysates)
Cui Y., Xiw S., Luan J., et al. Identification of the receptor tyrosine kinases (RTKs)-oriented functional targets of miR-206 by an antibody-based protein array. FEBS Lett. 2015 Jul 22;589(16):2131-5. doi: 10.1016/j.febslet.2015.06.026.
Species:
Human
Sample Type:
Cell Lysate (A549 cells with or without various MiR mimics, lung adenocarcinoma)
Qi W, Cooke LS, Stejskal A, Riley C, Croce KD, et al. MP470, a novel receptor tyrosine kinase inhibitor, in combination with Erlotinib inhibits the HER family/PI3K/Akt pathway and tumor growth in prostate cancer. BMC Cancer. 2009;9:142. doi:10.1186/1471-2407-9-142
Species:
Human
Sample Type:
Cell Lysate (Prostate cancer cell lysate)
Wenqing Q, Cooke LS, Stejskal A, Riley C, Croce KD, Saldanha JW, Bearss D, Mahadevan D. MP470, a novel receptor tyrosine, in combination with Erlotinib inhibits the HER family/PI3K/Akt pathway and tumor growth in prostate cancer. BMC Cancer 2009;9:142.
Species:
Human
Sample Type:
Cell Lysate (LNCaP cultured cell lysate)
Matsumoto M., Seike M., Noro R., et al. Control of the MYC-eIF4E axis plus mTOR inhibitor treatment in small cell lung cancer. BMC Cancer. 2015 Apr 9;15:241. doi: 10.1186/s12885-015-1202-4.
Species:
Human
Sample Type:
Cell Lysate (Small Cell Lung cancer cell lines (7 in total), treated with everolimus)
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Great productHighly informative setup that answers many initial questions and provides insights on future directions. The protocol is easy to follow and not very time consuming. The final results came out clear with high degree of reproducibility. Very low background and great signal to noise ratio. On the less positive side I would mention high cost of the kit. But given the fact the kit provides with great amount of data the one might consider the price factor as worthwhile.
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