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    Phospho-Stat 3 (Y705) and Total Stat 3 ELISA Kit

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    4 Citations
    This assay semi-quantitatively measures phosphorylated Stat 3 (Tyr705) and Total Stat 3 in lysate samples.
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    As low as $625.00
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    Catalog #:
    PEL-Stat3-Y705-T
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human, Mouse, Rat
    Accession Number P40763
    Gene Id 6774
    Gene Symbols STAT3|APRF
    Protein Name / Synonyms Signal transducer and activator of transcription 3 (Acute-phase response factor)
    Quantitative/Semi-Quantitative Semi-Quantitative
    Specificity The antibody pair provided in this kit recognizes human, mouse and rat Phospho-Stat3 (pTyr705) + pan Stat3.
    Compatible Sample Types Tissue Lysates, Cell Lysates
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Research Area Post-Translational Modifications, Phosphorylation, JAK/STAT Signaling
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage -20°C

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    Product Features

    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Other Materials Required
    • Distilled or deionized water
    • 100 ml and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 µl to 1 ml volumes
    • Adjustable 1-25 ml pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Protocol Outline
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 µl of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 µl of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Storage/Stability

    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

    Citations

    Hegazy N, Rezq S, Fahmy A. Mechanisms Involved in Superiority of Angiotensin Receptor Blockade over ACE Inhibition in Attenuating Neuropathic Pain Induced in Rats. Neurotherapeutics. 2020 Jul;17(3):1031-1047. doi: 10.1007/s13311-020-00912-8. PMID: 32804335; PMCID: PMC7609714.
    Species:
    Human
    Sample Type:
    Conditioned Media
    View Publication
    Tukaj S., Gruner D., Tukaj C., et al. Calcitriol exerts anti-inflammatory effects in keratinocytes treated with autoantibodies from a patient with bullous pemphigoid. J Eur Acad Dermatol Venereol. 2015 Jan 22. doi: 10.1111/jdv.12929
    Species:
    Human
    Sample Type:
    Cell Lysate (Human Keratinocyte cell cultures)
    View Publication
    van Oppen, Johan N., and Eugen J. Verspohl. "Impact of Bisphenol A (BPA) and Free Fatty Acids (FFA) on Th2 Cytokine Secretion from INS-1 Cells." Pharmacology & Pharmacy 4.05 (2013): 451.
    Species:
    Rat
    Sample Type:
    Conditioned Media (INS-1 Cells)
    View Publication
    Al-Maghrebi, May, et al. "Lutein modulates transcription dysregulation of adhesion molecules and spermatogenesis transcription factors induced by testicular ischemia reperfusion injury: it could be SAFE." Naunyn-Schmiedeberg's archives of pharmacology 389.5 (2016): 539-551.
    Species:
    Rat
    Sample Type:
    Tissue lysate (testicular tissue)
    View Publication

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