Human/Mouse MAPK Phosphorylation Array

Name: Human/Mouse MAPK Phosphorylation Array
Brand: AAH-MAPK-1
SKU: AAH-MAPK-1
Price: 342 USD
Availability: InStock
Rating: 98 (9 reviews)

Rating:

98 % of 100

9 Reviews

15 Citations

Detects 17 phosphorylated proteins in human or mouse samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

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Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Human, Mouse
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	17
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	Membrane
Method Of Detection	Chemiluminescence
Design Principle	Sandwich-based
Research Area	Post-Translational Modifications, Phosphorylation, Akt Signaling, HER/ErbB Signaling, IGF Signaling, JAK/STAT Signaling, MAPK Signaling, PI3K-AKT Signaling, Wnt/beta-Catenin Signaling, NFkB Signaling
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	-20°C

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Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Akt (P-Ser473) (PKB (P-Ser473)) Gene Symbol: AKT1 PKB RAC Gene ID: 207 Accession #: P31749 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Akt (S473) ELISA	CREB (P-Ser133) Gene Symbol: CREB1 Gene ID: 1385 Accession #: P16220 ELISA Kit: RayBio Human Phospho-CREB (S133) ELISA	ERK1 (P-T202/Y204)/ERK2 (P-Y185/Y187) Gene Symbol: MAPK1 ERK2 PRKM1 PRKM2 Gene ID: 5594 Accession #: P28482 ELISA Kit: RayBio Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (Y185/Y187) and Total Erk1/2 ELISA	GSK3a (P-Ser21) Gene Symbol: GSK3A Gene ID: 2931 Accession #: P49840 ELISA Kit: RayBio Human/Mouse/Rat Phospho-GSK3a (S21) ELISA	GSK3b (P-Ser9) Gene Symbol: GSK3B Gene ID: 2932 Accession #: P49841 ELISA Kit: RayBio Human Phospho-GSK3b (S9) ELISA
HSP27 (P-Ser82) Gene Symbol: HSPB1 HSP27 HSP28 Gene ID: 3315 Accession #: P04792 ELISA Kit: RayBio Human Phopspho-HSP27 (S82) ELISA	JNK (P-Thr183) Gene Symbol: MAPK8 JNK1 PRKM8 SAPK1 SAPK1C Gene ID: 5599 Accession #: P45983 ELISA Kit: RayBio Human/Mouse/Rat Phospho-JNK (T183/Y185) ELISA	MEK (P-Ser217/Ser221) Gene Symbol: MAP2K1 MEK1 PRKMK1 Gene ID: 5604 Accession #: Q02750 ELISA Kit: RayBio Human/Mouse/Rat Phospho-MEK1 (S217/S221) ELISA	MKK3 (P-Ser189) Gene Symbol: MAP2K3 MEK3 MKK3 PRKMK3 SKK2 Gene ID: 5606 Accession #: P46734 ELISA Kit: RayBio Human/Mouse/Rat Phospho-MKK3 (S189) ELISA	MKK6 (P-Ser207) Gene Symbol: MAP2K6 MEK6 MKK6 PRKMK6 SKK3 Gene ID: 5608 Accession #: P52564 ELISA Kit: RayBio Human/Mouse/Rat Phospho-MKK6 (S207) ELISA
MSK2 (P-Ser360) Gene Symbol: RPS6KA4 MSK2 Gene ID: 8986 Accession #: O75676 ELISA Kit: RayBio Human Phospho-MSK2 (S360) ELISA	mTOR (P-Ser2448) Gene Symbol: MTOR FRAP FRAP1 FRAP2 RAFT1 RAPT1 Gene ID: 2475 Accession #: P42345 ELISA Kit: RayBio Human Phospho-mTOR (S2448) ELISA	p38 (P-Thr180/Tyr182) Gene Symbol: MAPK14 CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A Gene ID: 1432 Accession #: Q16539 ELISA Kit: RayBio Human/Mouse Phospho-p38 alpha (T180/Y182) ELISA	P53 (P-Ser15) Gene Symbol: TP53 P53 Gene ID: 7157 Accession #: P04637 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P53 (S15) ELISA	P70S6K (P-Thr421/Ser424) Gene Symbol: RPS6KB1 STK14A Gene ID: 6198 Accession #: P23443 ELISA Kit: RayBio Human/Mouse/Rat Phospho-P70S6K (T421/S424) ELISA
RSK1 (P-Ser380) Gene Symbol: RPS6KA1 MAPKAPK1A RSK1 Gene ID: 6195 Accession #: Q15418 ELISA Kit: RayBio Human Phosph-RSK1 (S380) ELISA	RSK2 (P-Ser386) Gene Symbol: RPS6KA3 ISPK1 MAPKAPK1B RSK2 Gene ID: 10432 Accession #: P51812

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human/Mouse MAPK Phosphorylation Antibody Array C1 Membranes
Blocking Buffer
Detection Antibody Cocktail
1,000X HRP-Anti-Rabbit-IgG Concentrate
20X Wash Buffer I Concentrate
20X Wash Buffer II Concentrate
2X Cell Lysis Buffer Concentrate
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray w/ Lid
Protease Inhibitor Cocktail
100x Phosphatase Inhibitor Cocktail I
Phosphatase Inhibitor Cocktail II
Plastic Sheets
Array Map Template
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Detection Antibody Cocktail
Incubate with HRP-Conjugated anti-IgG
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Typical Data

Figure 1 - HeLa cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 250 nM PMA for 20 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Figure 2 - HepG2 cells were grown to 80% confluency and then serum starved overnight. Cells were either untreated (bottom panel) or treated (top panel) with 25 ng/mL of recombinant human IL-1β for 30 minutes. Data shown are from a 20 second exposure using a chemiluminescence imaging system.

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

Documents

Datasheet (AAH-MAPK-1.pdf)

SDS (AAH-MAPK-1.pdf)

Excel (AAH-MAPK-1.xls)

Citations

Yu S., Zhong Q., Chen R., et al. CRLF1 promotes malignant phenotypes of papillary thyroid carcinoma by activating the MAPK/ERK and PI3K/AKT pathways. Cell Death Dis. 2018 Mar 7;9(3):371. doi: 10.1038/s41419-018-0352-0.

Species:

Human

Sample Type:

Cell Lysate (Human thyroid cell lines, normal and cancerous; IHH-4, PTC, etc)

View Publication

Yu, Shi-Tong, et al. "CRLF1 promotes malignant phenotypes of papillary thyroid carcinoma by activating the MAPK/ERK and PI3K/AKT pathways." Cell death & disease 9.3 (2018): 1-13.

Species:

Human

Sample Type:

Cell Lysate

View Publication

Poling, Holly M., et al. "Mechanically induced development and maturation of human intestinal organoids in vivo." Nature biomedical engineering 2.6 (2018): 429-442.

Species:

Human

Sample Type:

Tissue Lysate

View Publication

Schuett J., Schuett H., Oberoi R., et al. NADPH oxidase NOX2 mediates TLR2/6-dependent release of GM-CSF from endothelial cells. FASEB J. 2017 Mar 8. pii: fj.201600729R. doi: 10.1096/fj.201600729R

Species:

Human

Sample Type:

Cell Lysate (Human umbilical cord endothelial cells)

View Publication

Schuett, Jutta, et al. "NADPH oxidase NOX2 mediates TLR2/6?dependent release of GM?CSF from endothelial cells." The FASEB Journal 31.6 (2017): 2612-2624.

Species:

Human

Sample Type:

Cell Lysate

View Publication

Yang Z, Zou W, Pan Y, Yong KT, Li L, Wang X, Liu D, Chen T, Xue D, Lin G. PEGylated CuInS2/ZnS quantum dots inhibit neurite outgrowth by downregulating the NGF/p75NTR/MAPK pathway. Ecotoxicol Environ Saf. 2021 Jan 1;207:111378. doi: 10.1016/j.ecoenv.2020.111378. Epub 2020 Oct 3. PMID: 33022524.

Species:

Rat

Sample Type:

Cell Lysate

View Publication

Dhani, Shanel, et al. "Fusaric acid alters Akt and ampk signalling in c57bl/6 mice brain tissue." Food and Chemical Toxicology (2020): 111252.

Species:

Mouse

Sample Type:

Tissue Lysate (brain tissue)

View Publication

Sarsour, Ehab H., et al. "Arachidonate 12-lipoxygenase and 12-hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer." Journal of Biological Chemistry 295.20 (2020): 6946-6957.

Species:

Human

Sample Type:

Cell Lysate (pancreatic cells)

View Publication

L'Huillier C, Jarbeau M, Achamrah N, et al. Glutamine, but not Branched-Chain Amino Acids, Restores Intestinal Barrier Function during Activity-Based Anorexia. Nutrients. 2019;11(6):1348. Published 2019 Jun 15. doi:10.3390/nu11061348

Species:

Mouse

Sample Type:

Tissue Lysate (Colon tissue )

View Publication

Hou J, Cui C, Kim S, Sung C, Choi C. Ginsenoside F1 suppresses astrocytic senescence-associated secretory phenotype. Chem Biol Interact. 2018;283:75-83. doi:10.1016/j.cbi.2018.02.002

Species:

Human

Sample Type:

Cell Lysate

View Publication

View All Publications For This Product

Reviews

Write a customer review

Great product

Rating
100%

This product was great. It was easy to use and provided great results.

Review by SI,

from UAMS,

on Sep 2, 2024
Great product

Rating
100%

This product is very easy and straightforward to use. I have been using this for a while now.

Review by stve,

from New York Medical College,

on Nov 10, 2023
Great product

Rating
100%

Showed robust results in lung cancer cell lines. Planning to order a couple more to use on other cell lines.

Review by YH,

from JHU,

on Nov 2, 2023
Great product

Rating
100%

Very easy-to-use, straightforward assay. Great for lung cancer samples.

Review by KC,

from Dana Farber Cancer Institute,

on Sep 29, 2023
Great product

Rating
100%

We compared the MAPK expression patterns in untreated and doxorubicin-treated mouse hearts. Heart muscle tissue extract were assayed at mid-range protein amounts loaded.

Review by Alexi Kiss,

from George Washington University,

on Dec 28, 2022
Great product

Rating
100%

1.Experimental conditions and sample typea. In vitro: Human breast cancer cell line (hBCC)b. In vivo: hBCC grew up in nude mouse bone + mouse bone (human/mouse protein mixed)Pros: pAKT1, pCREB, pGSK3b,p70S6K, pRSK1,2, pMEK1, pp38, and pMKK3,6 are good. The experimental process is very easy. Easy to reproduce the resultsCons: pERK1/2 is a signal relatively weak compared to the Cell signaling antibody.

Review by Yubei-BJH,

from Yale University,

on Mar 16, 2021
Great product

Rating
100%

This assay is very straightforward and provides good results for brain samples.

Review by Maria Rodrigues,

from Emory,

on Dec 7, 2020
Great product

Rating
100%

These arrays are what I was looking for. They are easy to use, very clean membranes with super sharp results. These arrays are a great second step for large scale analysis such RNAseq.

Review by Ehab Sarsour,

from The University of Iowa,

on May 5, 2019
Great product

Rating
80%

Easy to use and convenient way to look at multiple targets.

Review by Harsh Sharthiya,

from Midwestern University,

on Mar 25, 2019

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Featured products

Human/Mouse MAPK Phosphorylation Array

Product Description

Specifications

Risk-Free Guarantee

Amazon Gift Cards!

Product Features

Target Names

Application Notes

Typical Data

Storage/Stability

Documents

Citations

Yu S., Zhong Q., Chen R., et al. CRLF1 promotes malignant phenotypes of papillary thyroid carcinoma by activating the MAPK/ERK and PI3K/AKT pathways. Cell Death Dis. 2018 Mar 7;9(3):371. doi: 10.1038/s41419-018-0352-0.

Yu, Shi-Tong, et al. "CRLF1 promotes malignant phenotypes of papillary thyroid carcinoma by activating the MAPK/ERK and PI3K/AKT pathways." Cell death & disease 9.3 (2018): 1-13.

Poling, Holly M., et al. "Mechanically induced development and maturation of human intestinal organoids in vivo." Nature biomedical engineering 2.6 (2018): 429-442.

Schuett J., Schuett H., Oberoi R., et al. NADPH oxidase NOX2 mediates TLR2/6-dependent release of GM-CSF from endothelial cells. FASEB J. 2017 Mar 8. pii: fj.201600729R. doi: 10.1096/fj.201600729R

Schuett, Jutta, et al. "NADPH oxidase NOX2 mediates TLR2/6?dependent release of GM?CSF from endothelial cells." The FASEB Journal 31.6 (2017): 2612-2624.

Yang Z, Zou W, Pan Y, Yong KT, Li L, Wang X, Liu D, Chen T, Xue D, Lin G. PEGylated CuInS2/ZnS quantum dots inhibit neurite outgrowth by downregulating the NGF/p75NTR/MAPK pathway. Ecotoxicol Environ Saf. 2021 Jan 1;207:111378. doi: 10.1016/j.ecoenv.2020.111378. Epub 2020 Oct 3. PMID: 33022524.

Dhani, Shanel, et al. "Fusaric acid alters Akt and ampk signalling in c57bl/6 mice brain tissue." Food and Chemical Toxicology (2020): 111252.

Sarsour, Ehab H., et al. "Arachidonate 12-lipoxygenase and 12-hydroxyeicosatetraenoic acid contribute to stromal aging-induced progression of pancreatic cancer." Journal of Biological Chemistry 295.20 (2020): 6946-6957.

L'Huillier C, Jarbeau M, Achamrah N, et al. Glutamine, but not Branched-Chain Amino Acids, Restores Intestinal Barrier Function during Activity-Based Anorexia. Nutrients. 2019;11(6):1348. Published 2019 Jun 15. doi:10.3390/nu11061348

Hou J, Cui C, Kim S, Sung C, Choi C. Ginsenoside F1 suppresses astrocytic senescence-associated secretory phenotype. Chem Biol Interact. 2018;283:75-83. doi:10.1016/j.cbi.2018.02.002

Reviews

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