RayBio® C-Series Human Matrix Metalloproteinase Antibody Array 1 Kit. Detects 10 Human MMPs and TIMPs. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 10 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
MMP-1
| MMP-2
| MMP-3
| MMP-8
| MMP-9
|
MMP-10
| MMP-13
| TIMP-1
| TIMP-2
| TIMP-4
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Matrix Metalloproteinase Antibody Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007; 6: 1680-1693
Species:
Human
Sample Type:
Conditioned Media (conditioned or non-conditioned medium)
Smith GN Jr, Mickler EA, Payne KK, Lee J, et al. Lung Transplant Metalloproteinase Levels are Elevated Prior to Bronciolitis Obliterans Syndrome. Am J Transplant. 2007;7:1656-1661.
Species:
Human
Sample Type:
BAL Fluid (Bronchoalveolar lavage (BAL))
Thielitz A, Vetter R, Schultze B, Wrenger S, Simeoni L,et al.Inhibitors of Dipeptidyl Peptidase IV-Like Activity Mediate Antifibrotic Effects in Normal and Keloid-Derived Skin Fibroblasts. J Investig Dermatol. 2008;128:855-866
Species:
Human
Sample Type:
Conditioned Media
Tinaburri L, Valente C, Teson M, Minafò YA, Cordisco S, Guerra L, Dellambra E. The Secretome of Aged Fibroblasts Promotes EMT-Like Phenotype in Primary Keratinocytes from Elderly Donors through BDNF-TrkB Axis. J Invest Dermatol. 2020 Sep 12:S0022-202X(20)32062-5. doi: 10.1016/j.jid.2020.08.019. Epub ahead of print. PMID: 32931807.
Species:
Human
Sample Type:
Conditioned Media
Abdul Muneer P., Alikunju S., Szlachetka A., Haorah J. The mechanisms of cerebral vascular dysfunction and neuroinflammation by MMP-mediated degradation of VEGFR-2 in alcohol ingestion. Arterioscler Thromb Vasc Biol. 2012 May;32(5):1167-77. doi: 10.1161/ATVBAHA.112.247668
Species:
Human
Sample Type:
Conditioned Media (Human Brain endothelial cells treated with ethanol)
Cheng YC, Chiang HY, Cheng SJ, Chang HW, Li YJ, Shieh SY. Loss of the tumor suppressor BTG3 drives a pro-angiogenic tumor microenvironment through HIF-1 activation. Cell Death Dis. 2020 Dec 11;11(12):1046. doi: 10.1038/s41419-020-03248-5. PMID: 33311481; PMCID: PMC7732837.
Species:
Mouse
Sample Type:
Conditioned media
Del Carlo M, Schwartz D, Erickson EA, Loeser RF. Endogenous production of reactive oxygen species is required for stimulstion of human articular chondrocyte matrix metalloproteinase production by fibronectin fragments. Free Radical Biol Med. 2007;42:1350-1358.
Species:
Human
Sample Type:
Conditioned Media (chondrocyte cell-cultured medium)
Huang J., Yang B., Xiang T., et al. Diallyl disulfide inhibits growth and metastatic potential of human triple-negative breast cancer cells through inactivation of the ?-catenin signaling pathway. Mol Nutr Food Res. 2015 Jun;59(6):1063-75. doi: 10.1002/mnfr.201400668.
Species:
Human
Sample Type:
Conditioned Media (Triple negative breast cancer cell line TNBCs treated with Diallyl Disulfide)
Ichikawa T., Sugiura H., Koarai A., Minakata Y., Kikuchi T., Morishita Y., et al. TLR3 Activation Augments Matrix Metalloproteinase Production through Reactive Nitrogen Species Generation in Human Lung Fibroblasts. The Journal of Immunology April 23, 2014 1302919 [Epub Ahead of Print]
Species:
Human
Sample Type:
Conditioned Media (Human Fetal Lung Fibroblasts (HFL-1) and U2-OS Conditioned Media)
Salhi A., Farhadian J., Giles K., et al. RSK1 Activation Promotes Invasion in Nodular Melanoma. Am J Pathol. 2015 Mar;185(3):704-16. doi: 10.1016/j.ajpath.2014.11.021.
Species:
Human
Sample Type:
Tissue Lysate (Metastatic tumor lysates from melanoma patients)
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Great product2 8 well assays were used for an experiment and it worked well. I liked having the instructions available via web however, I used an old printed out manual that I received previously.
from Indiana University School of Dentistry,
on
Great productIn our experiment we are greatly satisfied with your kits. We were able to get clear and vivid pictures of MMP's and compare dots for different samples.from Stony Brook University (SUNY/Stony Brook Department of Ob/Gyn),
on
Great productWe have recently used the C-series array for the human MMPs on denatured protein isolated from skin extracts and it works great. I like to emphasize that even though the kit recommends the use of non-denatured proteins using their supplied buffer, this worked fine for us, which a plus point to use this array.The customer service and the scientific support team is excellent. We will definitely use their products again.Arupon
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