Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
HSPA1A, HTRA1, Human Agrin, IBP160 , IDH1, IDH3A, IFRD1 , IMP2, IGFBP-rp1, IGSF4B, Ihh, ILK, Inhibin beta, Integrin b1, Integrin beta 6, Intergrin a6, IQGAP1 , IQGAP2 , IRE1, IRS2, ISOC2, ITGB4BP, ITIH1, ITIH2, ITIH3, ITIH4 a, JAM-A, JARID2, Karyopherin beta 1, Keratin 36, Keratin 38, KHSRP, KIAA0319L, KIAA1468, KIAA1967, KIF5B , Kilon, KLK-B1, KMD4B, KMT2B, KRT31, KRT72, KRT73, KRT82, KRT85 N-terminal, KRTDAP, KRTHA3B, KSR1, LAD, LAF4, LAIR1, LAM b1, LAMA, Lamin A/C, Lamin B1, Lamin B2, Laminin 2 alpha, Laminin b2, Laminin gamma 1, LAMP, LAMP1, LAMP2, LAP3, LASP1, Latent TGF beta bp2, LCAT, LCMT2, LDHA, LDHB, LEDGF, LEKTI, LILRA3, LIMS1, LMAN2, LMW-PTP, LOK, LOX, LOXL1, LRP 4, LTA4H, LTBP4, Lubricin, LUZP1, LYPA1, Lysozyme, MAGI2, MAGP-2, MAN1, MANF, Mannosidase II, MAP1A, MAPRE1, MARCKS, Complement MASP3, MBD2 , MBP, MCAM, Mcl-1, MCM, MCM5 , MCMP2, MDH1, MDH2, ME1, Meprin alpha, Metallothionein, Metavinculin, MFAP4, MFI2, mGLUR5, MGP, Mimecan, MINPP1, MLCK, MMR, MMRN1, MN1, Moesin, MP1, MPCA, Myeloperoxidase, MRP 1, MSH6, mTOR, MUCDHL, Multimerin 2, MyBPC3, MYH2, MYH6, MYH7, MYHC, MYL12B , MYL3, MYO5A, Myoferlin , Myosin 18B, Myotrophin, NABC1, NAGLU, NAP1L1 , NAPRT1, NASP , NCAM2 , Nebulin, Nectin-1, Nectin-3, NEDD8, Neogenin, Nesprin2, Neurabin 1, N-Cadherin, Neurofibromin, Neurogranin, Neuropeptide B, Neuropilin-1, Neurotrimin, NF-M, Nidogen-2, NIT2, NME3, nNOS, Noelin, Non-muscle Actin, Non-muscle Myosin IIA, Notch-2, Notch-2 ICD, NPAS3, NPM1, NQO2, NT5C3, Nucleobindin 1, NUP98, OBCAM, OIT3, Olfactomedin-2, Ornithine Carbamoyltransferase , Orosomucoid 2, ORP150, OSBP1, OSCAR, Oncostatin M R beta, Osteoadherin, Oxytocin-neurophysin 1, p16 ARC , P20Sb3, p23, p39 , P4HB, p73, PA2G4, PABP, PACS1, PARVB, PCBP1, PCBP2, PCCA, PCDH7, PCDX8, PCK2 , PCMT1, PCNA, PCPE-1, Proprotein Convertase 9, PCYOX1, PDE1B, PDIA6, PDLIM1, PDLIM5, PDZD2, PEBP4, PEPD, PER1, Perilipin-3, Perilipin-1 , Periostin, Peroxiredoxin 2, Peroxiredoxin 3, Peroxiredoxin 5, PF4V1, PGAM1, PGAM2, PGD, PGDF, PGK-1, PGLS-C-t, PGM1, PGRPL, PHAP1, Phosphoserine Aminotransferase, PI3-Kinase C2 beta, pIgR, PIK3IP1, PIN, PISD, PKLR, PLA2G1B, Plakophilin 1, Plastin L, PLC-gamma 1 , Pleckstrin, Plectin, Plexin B1, Plexin B2, PLOD1, PLOD2, PLS3, Plxdc2, PNP, POR, PPCS, PPOX , PPP2R1B , PPP2R4, PRCP, PRDM13, PRDX 1, PRELP, PREP, PRG2, Prion protein PrP , Profilin 1, Properdin, Prosaposin, Prostaglandin D Synthase, Proteasome 20S a+b, Proteasome 20S alpha, Proteasome 20S alpha 5, Proteasome 20S b7, Proteasome 26S S5, Proteasome beta 1, Proteasome subunit alpha type 6, Proteasome subunit beta type 2, Proteasome subunit beta type 4, Coagulation Factor XIV, Protein Z, Prouroguanylin, PRSS23, PRSS3, Myeloblastin, PSMA1, PSMA2, PSMA4, PSMA7, PSMB5, PSMC3 , PSMD1, PSMD9 , PTEN, PTK 7, PTMA, PTP gamma, PTP kappa, PTP mu, PTPRS, PTPRZ, PYGL, PZP, QDPR, QPRT, Quiescin Q6, Rab7a, Ran, RanGAP1, RAP1AB, Rbm15, RCL , Reg1A, Reg3A, RHOC, RhoGDI, Ribonuclease A, Ribonuclease Inhibitor, Ribonuclease T2, RKIP, RNA Polymerase II, RNASE4, RNASE6, RPL10, RPL10A , RPL11, RPL12, RPL14 , RPL17, RPL22, RPL5, RPL7A, RPLP0 , RPS10, RPS11, RPS12, RPS19, RPS2, RPS20 , RPS23, RPS25, RPS28, RPS3, RPS5, RREB1, RSU1, S100 A1, S100 A11, S100 A7, S100 A9, S100P, SAA, SAA4a, Salivary alpha amylase, SAMSN1, SBP-1, SBSN, SDF4, SDNSF, SDPR, Secretogranin V, Semaphorin 6B, Semaphorin 7A, Semenogelin I, Semenogelin II, Serpin A11, Serpin A7, Serpin B3, Serpin B6, Serpin B8, Serpin F2, Serpin A10, Serpin B1, Serpin B4, Serpin E2, SerRS, SET, SEZ6L2, SF20, SH3BGRL, SH3BGRL3, SHANK1, SHC1, SHIP, SHMT1, SHP-1, Siglec-1, Siglec-14, SIM2 , SIRP beta 1, Six3, SLC38A10, SLITRK1, SLURP1, SMA, SMC4 , SMPD4, SOD1, SOD2, SOD3, SOD4, Somatostatin, SORD, SorLA, SOX4, SP-D, Spectrin beta-5, SPEN, SPINK7, SPTBN1, Src(1), SREC-II, STAT3, Stathmin 1, Sterol carrier protein 2, STI1, STOM, SUCLG1, SUMO3, Symplekin, SynCAM, Syntaxin 7, TAB182, TAGLN2, Talin1, Talin1&2, TAX1BP3 , TBCA, TCEB2, Tcf20, TCN1, TCP1 eta, Tenascin C, Tenascin X(1), TFF2(1), TGM3, Thioredoxin-1, Thimet Oligopeptidase, Thymosin b10, Titin, TLS, TMEM223, TOB2 , TOP2B, TPM4, Tripeptidyl-peptidase I, Transaldolase 1, Transketolase, Transthyretin, TRAP1, TRAP220, TRF 2, Triosephosphate isomerase, Tropomyosin 3, TRP-1, TRPS1, Trypsinogeb-2, TrypsinPan, Tryptophanyl, TSR2, TUBA6, TWF2, TXNDC4, TXNDC5, TXNRD2, UBE2D3, Ube2L3, UBE2N, Ubiquitin+1, UCH-L1, UFM 1, UGGT, UNC-13 Homolog D, UNC45A, UNC5H4, UPB1 , UQCRB, UQCRH , URB, URB2, UROC1, UROD, URP2, USP14 , USP5 , Uteroglobin, Utrophin, VAP-1, VAP-A, VCP, VDAC1 , Versican isoform V0, Vimentin B, VNN1, VSIG4, WDR1, WISP2, WNK2, YB1 , YY1, ZBTB4, ZC3H4-N-t, ZC3H8, ZDHHC18 , ZNF671, Zyxin
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Glass Slide(s)
- Spin Columns
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Streptavidin-Conjugated Fluor
- Adhesive Plastic Strips
- Labeling Buffer
- Lysis Buffer
- 30 ml Centrifuge Tube
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- 1 ml tube
- Pipettors, pipette tips and other common lab consumables
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Dry the glass slide
- Block array surface
- Incubate with Samples
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.