Human L3 Array, Glass Slide

RayBio® L-Series Human Antibody Array 3 Glass Slide Kit. Detects 500 Human Proteins. Suitable for serum, plasma and cell culture supernatants. (4 or 8 Sample Kit)

As low as $716.00
In stock
Only %1 left
Catalog #:
AAH-BLG-3
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Product Description

Specifications

Size 4 Sample Kit, 8 Sample Kit
Species Human
Quantitative/Semi-Quantitative Semi-Quantitative
Number of Targets Detected 500
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
Solid Support Glass Slide
Method Of Detection Fluorescence Laser Scanner
Design Principle Label-based
Shipping Type Blue ice
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Product Features

  • High density arrays
  • Unbiased detection through direct biotin labeling of samples
  • Low sample consumption
  • High detection sensitivity
  • Same day result
  • Affordable and simple to use

Target Names

14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, 53BP1, LAMR1, ABAT, ABCF1, ABI3BP, ACAA1, ACAA2, ACACA, ACAA, ACLP, ACLY, Aconitase 1, ACTBL2, ACTC1, Actinin alpha 1, ADAMDEC1, ADAS, ADH1B, ADH1C, ADH4, ADH5, ADM, Advillin-N-t, AFG3L2, AGA, Aggrecan, AGXT, AHNAK , Ahsp, AIF, AK2 , AKAP9 , AKR1B1, AKR1C3, AKR7A2, ALAD, Alanine Transaminase, Alcohol Dehydrogenase, Aldehyde Oxidase 1, ALDH16A1 , ALDH1A1, ALDH9A1, Alkaline Phosphatase, ALP, alpha 1,2 Mannosidase IA, alpha Actinin 4, Alpha Fodrin, alpha Glucosidase II, Alpha-synuclein, alpha Tubulin, AlphaA Crystallin, ALS, Als2 , ALS+B62:B1212CR1 , Aminoacylase-1, Androgen Receptor, ANGPTL6, ANGPTL8, ANK, Ankrd26, Annexin A1, Annexin A2, Annexin A6, Annexin V, ANP, Antithrombin 3, APA, APLP-1, APM2, Apo (a), APOA1BP, ApoF, ApoL1, ApoL2, ARFBP1, ARFGEF3, Argininosuccinate Lyase, ArgRS, ARP19, Arp2, ARP2/3, Arp3, ARPC2, ARPC3, ART3, Aminopeptidase PILS, ARX, ASH2L , Asialoglycoprotein Receptor 2, ASK1 , Aspartate Aminotransferase , Aspartyl Aminopeptidase, ASXL1, ATBF1, ATP5A, ATP5O, ATPB, B3GNT2, B4GalT1, B7-H2, B7-H3, bad, Band 3, BASP1, Bassoon, BAZ2B, BCHE, bcl-w, BCOR, beta 1 Spectrin , beta B1 Crystallin, Tubulin beta-1 chain, Tubulin beta-3 chain, BID, BIN2, BIRC6, BLMH, BLVRB, BMP-1, BPGM, BPIFB1, BPIL1, BRCA 2, BRD2, Brevican, Brg1, BRSK1, BTD, BTF3, C1Q, C1QA, C1QB, C1QR1, C1RL, C1S, C3orf75, C4.4A, C4BPA, C5b-9, C6 -N-terminal, C8G, C9orf40, CA1, CA150 , CA2, CA3, CACNB4, CAD , Cadherin-22, Cadherin-6, Caldesmon, CALML5, Calmodulin, Calpain 1, Calpain S1 , Calpastatin, Calretinin, Calumenin , CAP1, CapG, CAPZA1, Carboxypeptidase B2, CARHSP1, Caspase-14, Catalase, Cathelicidin, Cathepsin A, Cathepsin G, Cathepsin H, Cathepsin Z, CBS, CCDC126, CCDC25, CCT3, CD109, CD133, CD155, ADP-Ribosyl Cyclase 2, CD16, CD21, CD32, CD35, CD39L4, CD41, CD42b, CD48, CD5L, CD9 , CD98, CDA, CDC5L, CDK2 , CEACAM-8, CECR1, CENPF, CEP57, Carboxylesterase 1, CETP, Cezanne, CFHR 1, CFHR4, Complement Factor H-related 5, CFI, CFL1, Coagulation Factor VII, CHC17, Chitobiase, Chitotriosidase, CHORDC1, CHREBP, Chromogranin B, Chromogranin C, CIP29, CKB, CLIC1, CLIC4, CLIP170-N-t, Collectin-12, CLPS, CLTA, CNN2, CNOT1, CO4A2, COG4 , COL19A1, COL4A3, COL6A2, COL9A3, COLEC10, Collagen I alpha-1, Collagen III, Collagen IV alpha-6, Collagen IX, Collagen V, Collagen VI, Collagen X, Collagen XV alpha 1, Thrombospondin 5, Complement Factor B, Contactin-3, COPS8 , Corneodesmosin, Coronin 3, Cortactin, COTL1, Carboxypeptidase E, CPEB3, Carboxypeptidase M, CPN1, CPNE3, CPS1, Creatine Kinase MM, CRF21, CRHBP, CrkL, CRMP2, CRTAC1, CS, Ctip2, Cux2, Cyclophilin A, Cyclophilin B, Cystatin D, Cystatin E/M, Cystatin S, Cystatin SN, Cysteine-rich Protein 1, CYTL1, Cytochrome b5, Cytochrome C, Cytokeratin 1, Cytokeratin 10, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 16, Cytokeratin 17, Cytokeratin 20, Cytokeratin 3, Cytokeratin 4, Cytokeratin 5, Cytokeratin 9, D4-GDI, DAK, DAN, DARS2, DCI, DCXR, DDAH1, DDT, DDX3Y, DEFA6, DEP-1, Der p2, Dermcidin, Desmocollin-1, Desmocollin-2, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoplakin, Desmuslin, Destrin , DGK, DISC 1, DMGDH, DMRN9, Dopamine beta Hydroxylase, DOT1L, DPEP2, DPP3, DPPI, DRIL1, DSCAM, DSPG3, Dystroglycan, Ubiquitin-activating enzyme E1, ECHS1 , ECM-1, EEF1G , EEF2, EFEMP2 , EFTUD2, EHD1 , EHD3, EIF3S2, eIF4A1-N-terminal, eIF5A, ELAVL1 , EMILIN1 , EMSY, EN2, Endorepellin, ENO1, ENO1/2/3, ENSA, Envoplakin, Eosinophil derived neurotoxin, EPB41, EPCR, Ephrin-B1, Ephrin-B2, EPHX2, EPPK1, Eps 15, ERAB, Aminopeptidase LRAP, ERp29, ERp57, ERp72, ESD, ESR1, ETL, EVC2, Ezrin, Coagulation Factor XI, FABP5, Coagulation Factor IX, Coagulation Factor V, Coagulation Factor XII, Coagulation Factor XIII, FAM20C, FAM3C, Fascin, FASN, fast skeletal Myosin, FASTKD5, F-box protein 38, FBPase 2, FBPase 1, FCGBP, FDPS , FH, Fibrillin 1, Fibrinogen gamma chain, Fibrinogen-like 2, Fibrinopeptide B, Fibulin 3, Ficolin-2, Filamin A, Filamin B, Filamin C, FKBP12, FKBP25, FKBP51, FLG2, FOLR3, Frizzled 8, FRY, FSH, FTL, FUCA1, FUCA2, Fumarylacetoacetate hydrolase, G0/G1switch 2, G3BP , GALNT2, Catenin gamma, GAPHD, GARNL1 , GART, Gastrokine 1, GATM C-terminal, GBE1, GCDFP15, GCLC , GCSH , GDA, GDF7, GDI1, GDI2, Gephyrin, GFAP, GHRF, GIP, GLIPR2, GLRX1, G6PD, Glucosidase 2 subunit beta, GLUD1, Glutamyl hydrolase gamma , GSTO1, Glutathione Synthetase, Glycerol 3 Phosphate Dehydrogenase, GPV, Glyoxalase II, GM2A, GMF beta, GNB1, GNPTG, GOLM1, GOLPH4 , GOT2, GPCR GPR116, GPLD1, Grainyhead-like protein 1 homolog, Granzyme M, GRHPR, GRP, GSTM1, GSTP1, Guanylin, GULP1, H6PD, HABP2, HBZ, HCFC1, HDGF, HEG1, Hemoglobin, Hemoglobin A1c, Hemoglobin subunit beta, Hemoglobin subunit delta, Hemoglobin subunit gamma 2, HEXB, HGFA, Growth Hormone, hHR23b, HIBADH , HINT1, HIP1R , Histone H1.2, Histone H1.3, Histone H2A, Histone H2A.Z, Histone H2B K, Histone H3.3, Histone H4, HLA-C, HMGB1, HMGB2, HMGB3, HMGN2, HN1, HNF-3 alpha , hnRNP A1, hnRNP A2B1, hnRNP C1/C2, hnRNP G , hnRNP L, hnRNP M1-M4 , hnRNP U , Hornerin, HOXB3, HOXD11, HP1BP3, HPD, HPR, HPRT, HPRG, HRSP12, HSC70, HSP47

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Antibody Array Glass Slide(s)
  • Spin Columns
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Streptavidin-Conjugated Fluor
  • Adhesive Plastic Strips
  • Labeling Buffer
  • Lysis Buffer
  • 30 ml Centrifuge Tube
Other Materials Required
  • Distilled or de-ionized water
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • 1 ml tube
  • Pipettors, pipette tips and other common lab consumables
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline
  1. Purify Samples with Spin Column
  2. Biotinylate Samples
  3. Purify biotinylated samples using spin column
  4. Dry the glass slide
  5. Block array surface
  6. Incubate with Samples
  7. Incubate with Streptavidin-Conjugated Fluor
  8. Disassemble the glass slide
  9. Scan with a gene microarray laser scanner
  10. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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