Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
ACE / CD43, ACE-2, ACTH, ADFP, Adiponectin, Adipsin (Factor D), AgRP , AMPKa1 , Amylin, Angiopoietin 1, Angiopoietin 2, Angiotensinogen / Angiotensin II, Ang-like Factor, ANGPTL1, ANGPTL2, ANGPTL3, ANGPTL4 , Apelin Receptor , ApoB, ApoE, Axl, BDNF, bFGF, BMP-2 , BMP-3, BMP-3b / GDF-10, BMP-4 , BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA / ALK-3, BMPR-IB / ALK-6, BMPR-II, b-NGF, C3a des Arg, CART , CD137 (4-1BB), CD36, Clusterin, CNTF, C-peptide, CRP , Cystatin C, Dtk, EGF, EGFR, ENA-78, Endophin Beta, Epiregulin, E-selectin, ET-1 (Endothelin), FABP4, FAM3B, FAS / Apo-1, FGF-10, FGF-6, FSH , Galectin -1, Growth Hormone (Growth Hormone), Ghrelin, GITR, GITR Ligand, GLP-1 , Glucagon, Glut1, Glut2, Glut3, Glut5, Glutathione peroxidase 1, Glutathione peroxidase 3, GRO alpha, HCC4, HGF, HSD-1, ICAM1, IFN-gamma, IGF-1, IGF-1 sR, IGFBP-1, IGFBP-2, IGFBP-3, IGF-2, IL-1 R1, IL-1 R4, IL-1a, IL-1b, IL-1 ra, IL-6, IL-6 sR, IL-8, IL-10, IL-11, IL-12, IL-25 / IL-17E, INSL3, INSRR , Insulin, Insulin R (CD220), Leptin, Leptin R, LH (Luteinizing Hormone), LIF, LOX, Lymphotactin, MCP-1, MCP-3, M-CSF, MIF, MIP-1 alpha, MIP-1 beta, MIP-3b, MMP-2, MMP-9, MMP-11, MMP-19, MSHa , MSPa, Myostatin, NAIP, NeuroD1, Neurophilin-2, NGF R, NPY (Neuropeptide Y), Obestatin R (GPR-39), Orexin A, Orexin B, OSM, Osteocalcin, Osteonectin , Osteoprotegerin, PARC, PDGF, PDGF-AA, PDGF-AB, PDGF-C, PDGF-D, PEDF , Pentraxin-3, PPARg2 / NRIC3, Pref-1 , Prohibitin, Prolactin, PYY , RANTES, RBP4 , RELMb , Resistin, S100, S100 A8+A9, S100 A10, SAA , SDF-1, SEMA3A, Serotonin, Syndecan-3, TACE, TDAG51 , TECK, TGF alpha, TGF-b, Thrombospondin 1, Thrombospondin 2, Thrombospondin 4, TIMP-1, TIMP-2, TIMP-3, TIMP-4, Tissue factor (CD142), TLR2, TLR4, TNF alpha, sTNFRI, sTNFRII, TSG-6 , TSH , Vaspin, VCAM-1, VEGF-A, Visfatin/PBEF1, XEDAR
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Membranes
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Detection Buffer C
- Detection Buffer D
- Wash Buffer 1
- Wash Buffer 2
- HRP-Conjugated Streptavidin Concentrate
- Plastic Sheets
- Spin Columns
- Labeling Buffer
- Lysis Buffer
- Plastic Incubation Tray
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- Pipettors, pipette tips and other common lab consumables
- 2?5 ml tube
- 15 ml conical collection tube
- Tissue Paper, blotting paper or chromatography paper
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Block array membranes
- Incubate with Samples
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
