Human JAK/STAT Pathway Array C3
The Human JAK/STAT Pathway Array 3 is a membrane-based cytokine array for measuring 43 human protein biomarkers from the JAK/STAT pathway. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 43 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Research Area | JAK/STAT Signaling |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
|
Bcl-2
|
Cardiotrophin-1
(CT-1)
|
c-Myc
(MYC)
|
CNTF
|
CNTF R alpha
|
|
Common beta Chain
|
Erythropoietin
|
Erythropoietin R
|
GCSF R
(CD114)
|
GFAP
|
|
GM-CSF R alpha
|
Growth Hormone
|
IFN-alpha / beta R2
|
IFN-beta
|
IFN-gamma R1
|
|
IL-10 R alpha
|
IL-15 R alpha
|
IL-19
|
IL-20
|
|
|
IL-20 R beta
|
IL-21
|
IL-22BP
|
||
|
IL-23 R
|
IL-27 R alpha
|
IL-28Â R alpha
(IFN-lambda R1)
|
IL-3
|
|
|
IL-4 R alpha
|
IL-5 R alpha
|
IL-7 R alpha
(CD127)
|
Leptin R
|
LIF R alpha
|
|
Mcl-1
(Bcl2L3)
|
Oncostatin M
|
Oncostatin M R beta
(IL-31 R beta)
|
PDGF R alpha
|
Prolactin R
|
|
SHP-1
|
TSLP R
|
 |  | |
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human JAK/STAT Pathway Antibody Array C3 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
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