Human JAK/STAT Pathway Array C2
C-Series Human JAK/STAT Pathway Antibody Array 2. Detects Human protein biomarkers from the JAK/STAT pathway. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 39 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Solid Support | Membrane |
| Method Of Detection | Chemiluminescence |
| Design Principle | Sandwich-based |
| Research Area | JAK/STAT Signaling |
| Estimated Lead Time | 4-8 weeks |
| Shipping Type | Blue ice |
| Storage | -20°C |
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.
Learn More
Amazon Gift Cards!
$5 Amazon gift card in every kit box purchased.
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
EGF
|
EGFR
|
GCSF
|
GM-CSF
|
|
Growth Hormone
|
IFN-gamma
|
IL-10
|
IL-11
|
|
IL-12 p40
|
IL-12 p70
|
IL-13
|
IL-13 R alpha 1
|
IL-13 R alpha 2
|
IL-15
|
IL-2
|
IL-2 R alpha
|
IL-2 R beta (CD122)
|
IL-2 R gamma (Common gamma Chain)
|
IL-21 R
|
IL-23 p19
|
IL-28A (IFN-lambda 2)
|
IL-29 (IFN-lambda 1)
|
IL-4
|
IL-5
|
IL-6
|
IL-7
|
IL-9
|
|
Leptin
|
LIF
|
p21
|
PDGF R beta
|
PDGF-AA
|
PDGF-BB
|
Prolactin
|
Thrombopoietin (TPO)
|
TSLP
|
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human JAK/STAT Pathway Antibody Array C2 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Write Your Own Review