RayBio® Human IP-10 (CXCL10) ELISA Kit for cell culture supernatants, plasma, and serum samples.

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As low as $336.00 Regular Price $420.00
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Catalog #:
ELH-IP10
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species Human
Accession Number P02778
Gene Id 3627
Gene Symbols CXCL10|INP10|SCYB10
Protein Name / Synonyms C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10) [Cleaved into: CXCL10(1-73)]
Quantitative/Semi-Quantitative Quantitative
Specificity This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP- 2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Sensitivity 2.5 pg/ml

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Detection Range 8.23 pg/ml - 6000 pg/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage ≤-20°C

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Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Gao Q., Camous X., Lu Y., et al. Novel inflammatory markers associated with cognitive performance: Singapore Longitudinal Ageing Studies. Neurobiol Aging. 2016 Mar;39:140-6. doi: 10.1016/j.neurobiolaging.2015.12.002.
Species:
Human
Sample Type:
Plasma (Cognitive Performance)
Perisdsky Y., Hill J., Zhang M., et al. Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab. 2016 Apr;36(4):794-807. doi: 10.1177/0271678X15606149.
Species:
Human
Sample Type:
Conditioned Media (HIV innoculated brain periocytes)
Marozin S., Altonmonte J., Munoz-Alvarez K., et al. STAT3 inhibition reduces toxicity of oncolytic VSV and provides a potentially synergistic combination therapy for hepatocellular carcinoma. Cancer Gene Ther. 2015 Jun;22(6):317-25. doi: 10.1038/cgt.2015.23.
Species:
Human
Sample Type:
Conditioned Media (Carcinoma Cell lines treated with a Stat3 inhibitor or control)
Bratland, Eirik, Alexander Hellesen, and Eystein S. Husebye. "Induction of CXCL10 chemokine in adrenocortical cells by stimulation through toll-like receptor 3." Molecular and cellular endocrinology 365.1 (2013): 75-83.
Species:
Human
Sample Type:
Plasma (autoimmune Addison's disease patients)
Fujiwara S., Nagai H., Oniki S., et al. Interleukin (IL)-17 versus IL-27: opposite effects on tumor necrosis factor-?-mediated chemokine production in human keratinocytes. Exp Dermatol. 2012 Jan;21(1):70-2. doi: 10.1111/j.1600-0625.2011.01384.x
Species:
Human
Sample Type:
Conditioned Media (Normal human epidermal keratinocytes)
Vargas-Inchaustegui D., Hogg A., Tulliano G., et al.CXCL10 Production by Human Monocytes in Response to Leishmania braziliensis Infection. Infect. Immun. January 2010 vol. 78 no. 1 301-308
Species:
Human
Sample Type:
Conditioned Media (PBMC and macrophage cultures)
Ko TM., Kuo HC., Chang JS., et al. CXCL10/IP-10 is a Biomarker and Mediator for Kawasaki Disease. Circ Res. 2015 Feb 27;116(5):876-83. doi: 10.1161/CIRCRESAHA.116.305834.
Species:
Human
Sample Type:
Plasma (Kawasaki's Disease patients)
Hardy GAD, Sieg S, Rodriguez B, et al. Interferon-? is the primary plasma type-I IFN in HIV-1 infection and correlates with immune activation and disease markers. PLoS ONE. 2013;8(2):e56527.
Species:
Human
Sample Type:
Plasma (detection limit 8.00pg/ml)
Cakir, Mehtap, et al. "Serum CXCL10 levels and neuromuscular manifestations in patients with autoimmune thyroid diseases." Autoimmunity 44.6 (2011): 496-503.
Species:
Human
Sample Type:
Serum
Wu C, Lu X, Wang X, et al. Clinical symptoms, immune factors, and molecular characteristics of an adult male in Shenzhen, China infected with influenza virus H5N1. J Med Virol. 2013 May;85(5):760-768.
Species:
Human
Sample Type:
Serum
  1. Great product
    Rating
    100%
    The Human IP-10 ELISA was used to test the levels of secreted C-X-C Motif Chemokine Ligand 10 upon treatment of cancer cells with chemotherapeutics. Results were consistent.

    Review by

    from AUSTRALIS PHARMACEUTICALS,

    on

  2. Great product
    Rating
    100%
    Easy to follow and works exactly as expected.

    Review by

    from Biocogent, LLC,

    on

  3. Great product
    Rating
    100%
    I have been using the IP-10 ELISA assay for several months to measure IP-10 levels in human plasma and purified from blood. It has very low background and works well for my projects. Controls produce consistent OD450 values when stored as directed and used within expirations listed.

    Review by

    on

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