Human Inflammation Array Q1
Glass slide multiplex ELISA array quantitatively detects 10 human inflammatory factors.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Research Area | Inflammation, JAK/STAT Signaling, MAPK Signaling, NFkB Signaling |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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$5 Amazon gift card in every kit box purchased.
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-10
| IL-13
|
IL-4
| IL-6
| IL-8 (CXCL8)
| MCP-1 (CCL2)
| TNF alpha
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Inflammation Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Zhai Y, Zhong Z, Chen C-YA, Xia Z, Song L, Blackburn MR, Shyu A-B. Coordinated Changes in mRNA Turnover, Translation, and RNA Processing Bodies in Bronchial Epithelial Cells following Inflammatory Stimulation. Mol Cell Biol. 2008; 28(24):7414-7426.
Species:
Human
Sample Type:
N/A (Conditioned Media)
Wu B., Wang W., Zhan Y., et al. CXCL13, CCL4, and sTNFR as circulating inflammatory cytokine markers in primary and SLE-related autoimmune hemolytic anemia. J Transl Med. 2015 Apr 8;13:112. doi: 10.1186/s12967-015-0474-4.
Species:
Human
Sample Type:
Plasma (Autoimmune hemolytic anemia patients)
Modulation of Cytokine Release and Gene Expression by the Immunosuppressive Domain of gp41 of HIV-1
Species:
Human
Sample Type:
N/A (Conditioned Media)
Zhang C, Ding H, He M, Liu L, Liu L, Li G, et al. (2016) Comparison of Early Changes in Ocular Surface and Inflammatory Mediators between Femtosecond Lenticule Extraction and Small-Incision Lenticule Extraction. PLoS ONE 11(3): e0149503. doi:10.1371/journal.pone.0149503
Species:
Human
Sample Type:
Tears (femtosecond vs small incision lenticule extraction)
Provençal, Nadine, et al. "Childhood chronic physical aggression associates with adult cytokine levels in plasma." PLoS One 8.7 (2013): e69481.
Species:
Human
Sample Type:
Plasma (Childhood Chronic Physical Aggression Associates with Adult Cytokine Levels in Plasma)
Provençal, Nadine, et al. "Differential DNA methylation regions in cytokine and transcription factor genomic loci associate with childhood physical aggression." PLoS One 8.8 (2013): e71691.
Species:
Human
Sample Type:
Plasma
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good cytokine arrayeverything went well and the kit was easy to use.
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