Human IL-6 Immuno-PCR Assay Kit, BIQ-ELISA™
The Human Bead-based Immuno-Quantitative (BIQ) IL-6 ELISA Kit merges the specificity of sandwich-based ELISA, the sensitivity of real-time PCR, and the easy manipulation of microspheres to detect even the lowest levels of IL-6 in serum, plasma, and cell culture supernatant samples.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Human |
| Host Species | Human |
| Accession Number | P05231 |
| Gene Id | 3569 |
| Gene Symbols | IL6, IFNB2 |
| Protein Name / Synonyms | Interleukin-6 (IL-6) (B-cell stimulatory factor 2) (BSF-2) (CTL differentiation factor) (CDF) (Hybridoma growth factor) (Interferon beta-2) (IFN-beta-2) |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | The IL-6 antibodies used in the kit are specific to human IL-6. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Solid Support | 96-well Microplate |
| Method Of Detection | qPCR |
| Design Principle | Sandwich-based |
| Sensitivity | 0.03 pg/ml |
| Detection Range | 0.27 pg/ml - 200 pg/ml |
| Recommended Dilution (Serum/Plasma) | 2-10-fold |
| Estimated Lead Time | 3-5 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.
Learn More
Amazon Gift Cards!
$5 Amazon gift card in every kit box purchased.
Related Products
| Component | Size / Qty |
|---|---|
| 96-well V-bottom Plate | 1 plate |
| 96-well Plate Film | 1 plate |
| 96-well PCR Plate* | 1 plate |
| 1X Assay Diluent | 1 bottle (25 mL) |
| 20X Wash Buffer Concentrate | 1 bottle (30 mL) |
| Human IL-6 Standard | 1 vial |
| Human IL-6 Antibody Coated Beads | 1 vial (0.6 mL) |
| Oligonucleotide Conjugated Antibody | 1 vial (10 μL) |
| 1X Elution Buffer | 1 vial (5 mL) |
| 2X PCR Master Mix | 1 vial (1.05 mL) |
| Primer Mix | 1 vial (lyophilized) |
| PCR Plate Film | 1 piece |
*The PCR plate used is a 0.2 mL, non-skirted 96-well plate (Thermo Fisher, cat. no. AB0600). Please ensure compatibility with your PCR machine prior to purchase. For additional information contact technical support (techsupport@raybiotech.com).
Additional Materials Required
- Real-time PCR instrument, Bio-Rad or QuantStudio™ recommended
- Orbital 96-well plate shaker (with ability to reach 700 rpm)
- Microplate Washer (BioTek 405 TS Washer recommended) and a compatible 96-well V-bottom plate magnetic stand (ThermoFisher AM10050 recommended)
- Precision pipettes to deliver 1 μL to 1 mL volumes
- Pipette tips (barrier tips are recommended to reduce contamination)
- Adjustable 1-25 mL pipettes for reagent preparation
- Centrifuge that can spin 96 well plates at 500 g
- Nuclease-free water or deionized water
- 1.5 mL, 2 mL, or 5 mL microcentrifuge tubes
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add 5 μL of the bead and conjugate mix, and 5 μL of each standard and sample to each well. Incubate for 2.5 hours at room temperature while shaking.
- Add 40 μL Assay Diluent and wash the plate using a Microplate Washer.
- Add 40 μL Elution buffer to each well. Incubate for 10 minutes at room temperature.
- Prepare PCR/Primer Master Mix and pipette 15 μL into each well of the PCR plate.
- Transfer 5 μL supernatant from V-bottom plate to the PCR plate.
- Run real-time PCR.