RayBio® Human IGF-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

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As low as $420.00
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Catalog #:
ELH-IGF1
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species Human
Accession Number P05019
Gene Id 3479
Gene Symbols IGF1|IBP1
Protein Name / Synonyms Insulin-like growth factor I (IGF-I) (Mechano growth factor) (MGF) (Somatomedin-C)
Quantitative/Semi-Quantitative Quantitative
Specificity This antibody pair can only detect a free form of human IGF-1. It does not detect a complex, such as binding with IGFBPs. This IGF-1 ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Sensitivity 100 pg/ml

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Detection Range 0.1 ng/ml - 30 ng/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage ≤-20°C

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Target Background

Insulin-like-growth factor is a hormone structurally similar to insulin. It is central to the somatotropic axis acting downstream of growth hormone (GH) to promote tissue growth and development. IGF1 is synthesized in the liver, where its secretion is regulated by the pituitary secretion of GH. The actions of IGF1 are mediated through the IGF1R receptor to activate downstream signaling cascades such as PI3-kinase and mitogen-activated protein kinase pathways. Defects in the encoding gene can cause insulin-like growth factor deficiency - characterized by growth retardation, sensorineural deafness and intellectual disability.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
De Barros, Sandra, et al. "Aging-related decrease of human ASC angiogenic potential is reversed by hypoxia preconditioning through ROS production." Molecular Therapy 21.2 (2013): 399-408.
Species:
Human
Sample Type:
Conditioned Media (hASCs under normoxic and hypoxic conditions)
Kim S, Kang Y, Krueger CA, et al. Sequential delivery of BMP-2 and IGF-1 using a chitosan gel with gelatin microspheres enhances early osteoblastic differentiation. Acta Biomaterialia. 2012;8(5):1768-1777. doi:10.1016/j.actbio.2012.01.009.
Species:
Human
Sample Type:
Conditioned Media (Microspheres loaded with IGF1)
Pickard A, McDade SS, McFarland M, McCluggage WG, Wheeler CM, McCance DJ (2015) HPV16 Down-Regulates the Insulin-Like Growth Factor Binding Protein 2 to Promote Epithelial Invasion in Organotypic Cultures. PLoS Pathog 11(6): e1004988. doi:10.1371/journal.ppat.1004988
Species:
Human
Sample Type:
Conditioned Media (Conditioned form E6/7 Human foreskin keratinocytes)
Vinayak, Shaveta, et al. "A clinical trial of lovastatin for modification of biomarkers associated with breast cancer risk." Breast cancer research and treatment 142.2 (2013): 389-398.
Species:
Human
Sample Type:
Serum
Del Fante, Claudia, et al. "Conditioned Medium Originated From Lethally Irradiated Umbilical Cord Blood-Derived Mononuclear Cells Has Different Pro-Angiogenic Effects Over Mature and Progenitor Endothelial Cells In Vitro." (2013): 1068-1068.
Species:
Human
Sample Type:
Conditioned Media (monnuclear cells)
Dai Y, Ai B, Liu Y, Pascal LE, Wang Z, Dhir R, Sun X, Jiang Y. Abnormal expression of Rab27B in prostatic epithelial cells of benign prostatic hyperplasia alters intercellular communication. Int J Biochem Cell Biol. 2020 Dec 4;131:105898. doi: 10.1016/j.biocel.2020.105898. Epub ahead of print. PMID: 33285290.
Species:
Human
Sample Type:
Conditioned Media (cell culture media)
Stoppoloni D., et al. Effect of Glucosamine and its peptidyl-derivative on the production of extracellular matrix components by human primary chondrocytes. Osteoarthritis and Cartilage Sept 2014, epub ahead of print. http://dx.doi.org/10.1016/j.joca.2014.09.005
Species:
Human
Sample Type:
Conditioned Media
Krogh J., Rostrup E., Thomsen C., Elfving B., Videbech P., Nordentoft M. The effect of exercise on hippocampal volume and neurotrophines in patients with Major Depression - a randomized clinical trial. Journal of Affective Disorders Available online 23 April 2014 [Epub Ahead of Print]
Species:
Human
Sample Type:
Serum (Patient Serum)
Witberg, Guy et al. “Vascular endothelium function among male carriers of BRCA 1&2 germline mutation.” Oncotarget vol. 10,49 5041-5051. 20 Aug. 2019, doi:10.18632/oncotarget.27120
Species:
Human
Sample Type:
Plasma
Bai Y, Moeinzadeh S, Kim S, Park Y, Lui E, Tan H, Zhao W, Zhou X, Yang YP. Development of PLGA-PEG-COOH and gelatin-based microparticles dual delivery system and E-beam sterilization effects for controlled release of BMP-2 and IGF-1. Part Part Syst Charact. 2020 Oct;37(10):2000180. doi: 10.1002/ppsc.202000180. Epub 2020 Aug 18. PMID: 33384477; PMCID: PMC7771709.
Species:
Human
Sample Type:
Conditioned media (cell culture media)
  1. Great product
    Rating
    100%
    Excellent to make an analysis of protein secretome in cell differentiation processes.

    Review by

    from University of Puerto Rico,

    on

  2. Great product
    Rating
    100%
    Thanks for having great customer service! I needed to find some more information about the detection and capture antibody and when I called they were so helpful and informative!

    Review by

    from University of Florida,

    on

  3. Great product
    Rating
    100%
    We used this kit to measure the IGF in the extracellular vesicles from MSCs. It is easy to use and give a reliable result. The procedure is like other ELISA kits that I have used before. The kit is enough for two 96-well plates.

    Review by

    from North Carolina State University,

    on

  4. Great product
    Rating
    100%
    The kit works well and is reactive with His-tagged human IGF1, as well. Results are reproducible and the whole experiment can be performed in ~5 hours.

    Review by

    from University of Michigan,

    on

  5. Great product
    Rating
    100%
    The human IGF-1 ELISA kit (ELH-IGF1-1) is easy to work with and reproducible. Overall we are very happy with these kits.

    Review by

    from Lipscomb Univeristy,

    on

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