Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Human
Accession Number	P01579
Gene Id	3458
Gene Symbols	IFNG
Protein Name / Synonyms	Interferon gamma (IFN-gamma) (Immune interferon)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	15 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	15 pg/ml - 15000 pg/ml
Recommended Dilution (Serum/Plasma)	2 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Target Background

Interferon-gamma (IFN-gamma) is a pleiotropic cytokine with antiproliferative, pro-apoptotic and antitumor activity. It is a homodimer formed by the association of two 17 kDa polypeptides, that bind in an antiparallel manner to form a mature 50 kDa molecule. This symmetry allows for a single molecule to bind two receptors amplifying downstream signaling. IFN-gamma is secreted by CD4 T helper type 1 cells and CD8 cytotoxic cells, γδ T cells, NK cells and to a lesser extent by NK T cells, B cells and APC cells. Its expression is induced by cytokines such as IL-12, IL-15, IL-18 and type I IFN. The effects of IFN-gamma are mediated through the activation of the JAK/STAT pathway which modulates the transcription of many genes. IFN-gamma signaling plays a role in host defense, immune surveillance, establishing adaptive immunity and in regulating inflammation, apoptosis and the cell cycle.

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4^°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20^°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4^°C for up to 6 months. For extended storage, it is recommended to store at -80^°C.

Documents

Datasheet (ELH-IFNg.pdf)

Datasheet (ELH-IFNg-CL.pdf)

SDS (ELH-IFNg.pdf)

SDS (ELH-IFNg-CL.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Kundu-Raychaudhuri S., et al. Kv1.3 in psoriatic disease: PAP-1, a small molecule inhibitor of Kv1.3 is effective in the SCID mouse psoriasis e Xenograft model. Journal of Autoimmunity Aug 2014. epub ahead of print. http://dx.doi.org/10.1016/j.jaut.2014.07.003

Species:

Human

Sample Type:

Conditioned Media (CD3 activated T cells with CD3/28)

View Publication

Fermoselle C., Rabinovich R., Ausin P., et al. Does oxidative stress modulate limb muscle atrophy in severe COPD patients? Eur Respir J. 2012 Oct;40(4):851-62.

Species:

Human

Sample Type:

Tissue Lysate (Quadricep Biopsies from COPD patients)

View Publication

Liu Y., Liao J., Zhao M., et al. Increased expression of TLR2 in CD4(+) T cells from SLE patients enhances immune reactivity and promotes IL-17 expression through histone modifications. Eur J Immunol. 2015 Sep;45(9):2683-93. doi: 10.1002/eji.201445219.

Species:

Human

Sample Type:

Conditioned Media (CD4 T cells with a TLR2 gene expression)

View Publication

Khalid M., Yu H., Sauter D., et al. Efficient Nef-mediated downmodulation of TCR-CD3 and CD28 is associated with high CD4+ T cell counts in viremic HIV-2 infection. J Virol. 2012 May;86(9):4906-20. doi: 10.1128/JVI.06856-11

Species:

Human

Sample Type:

Conditioned Media (Human PBMCs infected with HIV constructs)

View Publication

Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR

Species:

Human

Sample Type:

Plasma (Diabetic Patient Samples)

View Publication

Sambataro M, Sambado L, Trevisiol E, Cacciatore M, Furlan A, Stefani PM, Seganfreddo E, Durante E, Conte S, Della Bella S, Paccagnella A, Dei Tos AP. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Jul;32(7):3742-3751. doi: 10.1096/fj.201701279RR. Epub 2018 Feb 12. PMID: 29436863.

Species:

Human

Sample Type:

Plasma (diabetes and NC patients)

View Publication

Tammali, Ravinder, et al. "Inhibition of aldose reductase prevents angiogenesis in vitro and in vivo." Angiogenesis 14.2 (2011): 209-221.

Species:

Human

Sample Type:

Conditioned Media

View Publication

ABDEL?HAMID, Mahmoud F., et al. "Serum levels of interleukin?8, tumor necrosis factor?? and ??interferon in Egyptian psoriatic patients and correlation with disease severity." The Journal of dermatology 38.5 (2011): 442-446.

Species:

Human

Sample Type:

Serum

View Publication

Yu H, Usmani SM, Borch A, et al. The efficiency of Vpx-mediated SAMHD1 antagonism does not correlate with the potency of viral control in HIV-2-infected individuals. Retrovirology. 2013;10:27.

Species:

Human

Sample Type:

Conditioned Media (HIV-infected dendritic cells)

View Publication

Abdallah M., et al. Serum Th1/Th2 and Macrophage Lineage Cytokines in Leprosy; Correlation with Circulating CD4+ CD25highFoxP3+ T-regs Cells. Experimental Dermatology, August 2014 Accepted Article, doi:10.1111/exd.12529

Species:

Human

Sample Type:

Serum

View Publication

View All Publications For This Product

Reviews

Write a customer review

Human IFN-gamma ELISA Assay Kit

Rating
100%

We used this kit to check the IFN-gamma levels in human melanoma cell lines. It is very sensitive and the instructions are easy to follow. But it took 5-6 hours for the whole process. We are very satisfied with this product.

Review by Teresa,

from Thomas Jefferson University,

on Apr 25, 2024
Excellent kit

Rating
100%

This kit is easy to use and the instructions are straightforward. It takes about 5 hours for the whole protocol. The preparation of the reagents takes some time. But you can start with the first incubation which is 2.5 hours first, then prepare the rest of the reagents during that incubation time. The result is pretty good with the standard compound provided in the kit to make a standard curve. It is on 30% OFF sale now. I just bought another two-plate kit. Hurry! :)

Review by TZ,

from Thomas Jefferson University,

on Apr 18, 2024
Great product

Rating
100%

Well trusted material for my analysis

Review by Carlos A. Barreto Gamarra,

from University of Puerto Rico,

on Sep 1, 2022
Great product

Rating
80%

we used this kit to detect IFN gamma in iPSC-derived CNS cells. it works fine. it took about 4.5 hours to complete the assay which is reasonable.

Review by Ming,

from Mayo Clinic,

on May 11, 2022
Great product

Rating
60%

Plasma & Urine were run at 2-fold dilution. Urine samples were BLQ. Plasma samples had very low concentration. Our samples and too low of a concentration to be measured by this assay.

Review by Anonymous,

from University of Michigan,

on Mar 14, 2022
Great product

Rating
100%

This ELISA kit was used for quantification of IFN-gamma in the supernatants of SEB-stimulated peripheral blood mononuclear cells in combination with conventional chemotherapeutics. The variability of the technical replicates was low and the data obtained very repetitive.

Review by MARINA K,

from KYNAN Pharma,

on Jul 17, 2021
Great product

Rating
100%

This human IFNg ELISA kit works very well. Steps included in the manufacturer's protocol were easy to follow and all the necessary materials were provided. The snap wells come in handy when running multiple experiments in small batches for optimization of the sample. We ran human plasma samples and the standards ran evenly and the detection concentration fell within the provided range which is rare for quantifying IFNg levels in human samples.

Review by Shreya Deb,

from University of Illinois at Chicago,

on Oct 31, 2020
Great product

Rating
80%

This is the first time I've run an Interferon-g ELISA and I'm very happy with the results. I typically use the ELISpot platform and I'm happy that the results with this kit are far superior- very little background and fairly sensitive with the detection. I may try the newer version (with the qRT-PCR) readout for subsequent experiments to compare between kits.

Review by Aish S,

from Mayo Clinic,

on Jan 1, 2020
Great product

Rating
80%

This human IFNg ELISA kit is good. Steps were clear and all necessary materials were provided. Since we ran multiple smaller experiments, snap wells were really handy. We used BAL from mice lungs and though the standard ran evenly, samples showed low IFNg levels.

Review by Hesper Wong,

from University of Pittsburgh,

on Jul 23, 2019
Great product

Rating
80%

The human IFNg ELISA kit is easy to follow and reproducible. We used the kit with IFNg-secreting human cell lines following the manufacturer's protocol after the cells were treated with LPS. The supernatant was used to detect the IFNg. The kit worked perfectly when detecting high content of IFNg. However, when the IFNg content is low in the samples, more repeats may be needed as the sensitivity or accuracy of the kit is becoming low, too. Overall, the kit works well in detecting IFNg.

Review by Rosa Ignacio,

on Jan 24, 2018

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Human IFN-gamma ELISA Kit