Name: Human Cytokine Array GS440
Brand: GSH-CAA-440
SKU: GSH-CAA-440
Price: 2033 USD
Availability: InStock

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Product Description

Specifications

Size	16 Sample Kit, 32 Sample Kit, 64 Sample Kit
Species	Human
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	440
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	Glass Slide
Method Of Detection	Fluorescence Laser Scanner
Design Principle	Sandwich-based
Shipping Type	Blue ice

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Introduction

RayBio^® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody^® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 &mirco;L per array).

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

2B4 (CD244), 4-1BB (TNFRSF9/CD137), 6Ckine (CCL21), ACE-2, Activin A, ADAM-12, ADAM-8, ADAM-9, ADAMTS-13, Adiponectin (ACRP30), Adipsin (Complement Factor D), aFGF (FGF-1), Aggrecan, AgRP, Albumin, ALCAM (CD166), ALK-6 (BMPR-IB), Alpha-fetoprotein, AMICA, Amphiregulin, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensinogen (SERPINA8), ANGPTL3, ANGPTL4, APRIL, AXL, B7-H1 (CD274), B7-H3 (CD276), BAFF, BCAM, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, Betacellulin (BTC), beta-NGF, bFGF, BLC (CXCL13), BMP-2, BMP-4, BMP-5, BMP-7, BMP-9, BMPR-IA (ALK-3), BMPR-II, C5a, CA125, CA15-3, CA19-9, CA9, Cadherin-11, Cadherin-13, Cadherin-4, Cathepsin B (Pro-Cathepsin B), Cathepsin L, Cathepsin S, CCL28 (MEC), CD14, CD147 (EMMPRIN), CD155 (PVR), CD163, CD200, CD229 (SLAMF3), CD23, CD26 (DPPIV), CD27 (TNFRSF7), CD30 (TNFRSF8), CD40 Ligand (TNFSF5), CD40 (TNFRSF5), CD48 (SLAMF2), CD58 (LFA-3), CD6, CD80 (B7-1), CD84 (SLAMF5), CD97, CD99, CEA (CEACAM-5), CEA (CEACAM-5), CEACAM-1, Chemerin, CHI3L1, CHL-1 (L1CAM-2), Ck beta 8-1 (CCL23/MPIF-1), Clusterin, CNTF, Coagulation Factor XIV (Protein C), Contactin-2 (TAG1), Cripto-1, CRP (C-Reactive Protein), CRTAM, CTACK (CCL27), CTLA-4 (CD152), CXCL14 (BRAK), CXCL16, Cystatin A, Cystatin B, Cystatin C, Cystatin E/M, DAN, DcR3, Decorin, Desmoglein-2, DKK-1, DKK-3, DKK-4, DLL1, DNAM-1 (CD226), DR3 (TNFRSF25), DR6 (TNFRSF21), Dtk, E-Cadherin, EDA-A2, EGF, EGFR, EG-VEGF (PK1), ENA-78 (CXCL5), Endoglin (CD105), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), ErbB2, ErbB3, ErbB4 (HER4), Erythropoietin R, ESAM, E-Selectin, FABP2, FAP, Fas Ligand (TNFSF6), Fas (TNFRSF6/Apo-1), Fc gamma RIIB/C (CD32B/C), Ferritin, Fetuin A, FGF-19, FGF-21, FGF-4, FGF-6, FGF-7 (KGF), FGF-9, FLRG, Flt-3 Ligand, FOLH1 (PSMA), Follistatin, Follistatin-like 1, FOLR1, Fractalkine (CX3CL1), FSH, Furin, Galectin-1, Galectin-2, Galectin-3, Galectin-7, Galectin-9, Gas 1, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, GCSF R (CD114), GDF-15, GDNF, GITR Ligand (TNFSF18), GITR (TNFRSF18), GM-CSF, gp130 (sgp130), Granulysin (NKG5/LAG-2), GRO alpha/beta/gamma, GRO alpha (CXCL1), Growth Hormone, HAI-2, HB-EGF, HCC-1 (CCL14), HCC-4 (CCL16), hCG beta, HGF, HGFR, HVEM (TNFRSF14), I-309 (TCA-3/CCL1), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), ICOS, IFN-alpha / beta R2, IFN-gamma, IGF-1, IGF-1 R, IGF-2, IGF-2 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-1 R1, IL-1 R2, IL-1 R3 (IL-1 R Acp), IL-1 R4 (ST2), IL-1 R5 (IL-18 R alpha), IL-1 R6 (IL-1 Rrp2), IL-1 Ra (IL-1 F3), IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 1 (IL-13 R1), IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17A, IL-17B, IL-17C, IL-17E (IL-25), IL-17F, IL-18, IL-18 BP alpha, IL-18 R beta (AcPL), IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma (Common gamma Chain), IL-20, IL-21, IL-21 R, IL-23, IL-24, IL-27, IL-28A (IFN-lambda 2), IL-29 (IFN-lambda 1), IL-3, IL-31, IL-32 alpha, IL-33 (IL-1 F11), IL-34, IL-36 alpha (IL-1 F6), IL-36 beta (IL-1 F8), IL-36 gamma (IL-1 F9), IL-36 Ra (IL-1 F5), IL-37 (IL-1 F7), IL-38 (IL-1 F10), IL-4, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-8 (CXCL8), IL-9, Insulin, IP-10 (CXCL10), I-TAC (CXCL11), JAM-A (CD321/F11R), JAM-B (VE-JAM/CD322), Kallikrein 14, Kallikrein 5, LAG-3, LAP/TGF beta 1 (TGF beta 1), Layilin, LDL R, Legumain, Leptin, Leptin R, LIF, LIGHT (TNFSF14), LIMPII, Lipocalin-2 (NGAL), LOX-1, LRIG3, LRP-6, L-Selectin (CD62L), Lymphotactin (XCL1), LYVE-1, Marapsin, MBL, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MCP-4 (CCL13), M-CSF, M-CSF R, MDC (CCL22), MEPE (OF45), Mer, MICA, MICB, Midkine, MIF, MIG (CXCL9), MIP-1 alpha (CCL3), MIP-1 beta (CCL4), MIP-1 delta (CCL15), MIP-3 alpha (CCL20), MIP-3 beta (CCL19), MMP-1, MMP-10, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MPIF-1 (CCL23), MSP alpha/beta, NAP-2 (PPBP/CXCL7), NCAM-1 (CD56), Nectin-4, Neprilysin, NGFR (TNFRSF16), Nidogen-1, Notch-1, NOV (CCN3), NrCAM, NRG1-beta 1 (HRG1-beta 1), NSE, NT-3, NT-4, Oncostatin M, Osteoactivin (GPNMB), Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PAI-1, PARC (CCL18), P-Cadherin, PD-1, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PECAM-1, Pentraxin-3 (TSG-14), Pepsinogen 1, Periostin, Persephin, PGRPs, Platelet Factor 4 (CXCL4), PLGF, Pref-1 (DLK-1/FA1), Procalcitonin, Prolactin, Prostasin, PSA-free, RAGE, RANK (TNFRSF11A), RANTES (CCL5), RBP4, Renin, Resistin, RGM-B, ROBO3, S100 A8, SCF, SCF R (CD117/c-kit), SDF-1 alpha (CXCL12 alpha), SDF-1 beta (CXCL12 beta), SERPINA4, sFRP-3, SIGIRR, Siglec-10, Siglec-5 (CD170), Siglec-7 (CD328), Siglec-9, SLAM (CD150), Sonic Hedgehog N-Terminal (Shh-N), SOST, SP-D, Syndecan-1, Syndecan-3, Syndecan-4, TACE, TACI (TNFRSF13B), TARC (CCL17), TECK (CCL25), Testican 2 (SPOCK2), TFPI, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta R3, Thrombomodulin, Thrombopoietin (TPO), Thrombospondin 1, Thrombospondin 2, Thrombospondin 5 (COMP), Thyroglobulin, Tie-1, Tie-2, TIM-1 (KIM-1), TIM-3 (KIM-3), TIMP-1, TIMP-2, TIMP-4, TLR2, TLR4, TNF alpha, TNF beta (TNFSF1B), TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRAIL R1 (TNFRSF10A/DR4), TRAIL R2 (TNFRSF10B/DR5), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRAIL (TNFSF10), TRANCE (TNFSF11), Transferrin, Transferrin, Trappin-2, TREM-1, TROP1 (EpCAM), Troponin I, TSH, TSLP, TWEAK (TNFSF12), ULBP-1, ULBP-2 (ALCAN-alpha/N2DL2), uPA, uPAR, VCAM-1 (CD106), VE-Cadherin (CDH5), VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, WIF-1, WISP-1 (CCN4), XEDAR

Application Notes

Suggested Application

Multiplexed Protein Detection
Biomarker Screening
Identifying Key Factors
Confirming Biological Process
Biomarker Validation
Validation of Antibody Array Results
Quantitative Protein Detection
Establishing Normal Range

Kit Components

Human Cytokine Array Q440 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
30 ml Centrifuge Tube
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.