Name: Human Cytokine Array GS1200
Brand: GSH-CAA-1200
SKU: GSH-CAA-1200
Price: 5040 USD
Availability: InStock

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Product Description

Specifications

Size	16 Sample Kit, 32 Sample Kit, 64 Sample Kit
Species	Human
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	1200
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
Solid Support	Glass Slide
Method Of Detection	Fluorescence Laser Scanner
Design Principle	Sandwich-based
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	-20°C

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Introduction

RayBio® G-Series Arrays are glass slide-based antibody arrays which allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid. Like a traditional sandwich-based ELISA, this array uses a matched pair of cytokine-specific antibodies for detection. Like the Quantibody® Multiplex ELISA Arrays, G-Series utilizes a highly sensitive and stable fluorescent readout which can be detected by most laser fluorescent scanner systems (see below for more info). After capturing the spot densities with a laser scanner, normalization of the raw data can be easily calculated by the researcher, or by a quick copy-paste into our excel-based Analysis Tool software.

This array as well as all catalog numbers beginning with 'GS' differ from the classic G-Series Arrays in a few important ways. First, each capture antibody is printed in quadruplicate instead of duplicate, delivering higher precision. Secondly, this array features the same antibody panels used in our Quantibody Arrays, allowing a seamless transition to our quantitative multiplex assay platform. Lastly, all 16 wells are spotted as sub-arrays, delivering easy handling of 16 samples simultaneously while consuming low sample volumes (10 - 100 µl per array).

Product Features

High sensitivity (pg/mL to ng/mL levels)
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 50 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Test 16 samples on each slide
Data analysis software provides one-step computation

Target Names

15-PGDH, 17-beta-HSD1, 2B4, 4-1BB, 4-1BB Ligand, 5'-Nucleotidase, 6Ckine, ACE, ACE-2, ACP6, Activin A, Activin AB, Activin C, Activin R1A, Activin R1B, Activin R2A, Activin R2B, ADA, ADAM-12, ADAM-22, ADAM-23, ADAM-8, ADAM-9, ADAMTS-13, ADAMTSL-1...View all 1200 human target proteins

Adiponectin, Adipsin, ADP-Ribosyl Cyclase 2, aFGF, Aggrecan, AgRP, AHCY, AIF, AKR1C4, Albumin, ALCAM, ALK-1, ALK-6, Alkaline Phosphatase, Alpha-fetoprotein, Alpha-Galactosidase A, AMICA, AmiGO, AmiGO2, Aminopeptidase LRAP, Aminopeptidase N, Aminopeptidase P2, Amnionless, Amphiregulin, AMSH, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiostatin, Angiotensinogen, ANGPTL2, ANGPTL3, ANGPTL4, ANGPTL7, Annexin A1, Annexin A4, Annexin A5, APE1, APRIL, Arginase 1, ARNT, Artemin, Arylsulfatase A, Arylsulfatase B, ASAH2, ASAHL, ASAM, Attractin, AXL, Azurocidin, B3GNT2, B4GalT1, B7-H1, B7-H2, B7-H3, B7-H4, BAFF, BAFF R, BAI1, BAI3, BAK, BAMBI, BATF3, bax, BCAM, BCL-10, BCL-2, bcl-w, BCMA, BDNF, beta IG-H3, Beta-2 Microglobulin, Betacellulin, Beta-glucuronidase, beta-NGF, bFGF, BID, Biglycan, BLAME, BLC, BLMH, BMP-2, BMP-4, BMP-5, BMP-7, BMP-8, BMP-9, BMPR-IA, BMPR-II, BNIP3, BOC, Bora, Brachyury, Brevican, Brorin, BTLA, C1q R1, C1qTNF1, C1qTNF4, C1qTNF9, C2, C5a, CA12, CA125, CA13, CA14, CA15-3, CA19-9, CA2, CA4, CA5A, CA5B, CA6, CA72-4, CA8, CA9, Cadherin-11, Cadherin-13, Cadherin-17, Cadherin-4, Cadherin-6, Calcitonin, Calreticulin, Calreticulin-2, Calsyntenin-1, CANT1, Carboxylesterase 1, Carboxylesterase 2, Carboxypeptidase A1, Carboxypeptidase A2, Carboxypeptidase B1, Carboxypeptidase E, Carboxypeptidase M, Cardiotrophin-1, Carnosine Dipeptidase-1, Caspase-1, Caspase-3, Caspase-7, Caspase-8, Caspase-9, Caspr2, Catalase, Cathepsin A, Cathepsin B, Cathepsin C, Cathepsin D, Cathepsin E, Cathepsin F, Cathepsin H, Cathepsin L, Cathepsin S, Cathepsin V, Cathepsin Z, Caveolin-2, CCL28, CD109, CD14, CD147, CD155, CD160, CD163, CD177, CD2, CD200, CD200 R1, CD229, CD23, CD26, CD27, CD27 Ligand, CD28, CD286, CD30, CD300a, CD300c, CD300e, CD300f, CD314, CD320, CD34, CD35, CD36, CD39L2, CD39L3, CD39L4, CD4, CD40, CD40 Ligand, CD42b, CD44, CD47, CD48, CD5, CD51, CD58, CD6, CD69, CD7, CD79 alpha, CD80, CD83, CD84, CD86, CD96 Isoform 2, CD97, CD99, CD99-L2, CDC25B, CDK5 Activator 1, CDNF, CDO, CDX4, CEA, CEA Variant, CEACAM-1, CEACAM-3, Cerberus 1, Chemerin, CHI3L1, Chitotriosidase, CHL-1, CHMP2B, Chordin-like 2, CHST1, CHST2, CHST3, CHST4, cIAP-1, cIAP-2, CIB1, CILP-1, Ck beta 8-1, CLEC-1, CLEC10A, CLEC-2, CLEC4D, CLEC9A, Clusterin, Clusterin-like protein 1, CMG-2, c-Myc, CNTF, CNTF R alpha, Coagulation Factor III, Coagulation Factor VII, Coagulation Factor X, Coagulation Factor XIV, Cochlin, COCO, Collagen alpha-1(XIII) chain, Collectin-12, Common beta Chain, Complement MASP3, Complexin-1, COMT, Contactin-1, Contactin-2, Contactin-3, Contactin-4, Contactin-5, Cortactin, C-Peptide, CREG, CRELD2, CRIM1, Cripto-1, CRISP-2, CrkL, CRP, CRTAC1, CRTAM, CTACK, CTLA-4, CTRC, C-type Natriuretic Peptide, CXADR, CXCL14, CXCL16, CXCL5, Cyclin E1, Cyclophilin A, Cyr61, Cystatin A, Cystatin B, Cystatin C, Cystatin D, Cystatin E/M, Cystatin S, Cystatin SA, Cystatin SN, Cytokeratin 18, Cytokeratin 19, Cytokeratin 8, DAB2, DAN, DAPP1, DBH, DCBLD2, DC-LAMP, DcR3, DC-SIGN, DC-SIGNR, DDR1, Decorin, Dectin-1, Dectin-2, DEFB-3, Desmin, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoglein-3, DFF45, DKK-1, Dkk-3, Dkk-4, DLEC, DLL1, DLL4, DNAM-1, DNER, DNMT3A, Doc2-alpha, Dopa Decarboxylase, DPPII, DR3, DR6, DRAK1, Draxin, DSCAM, DSCAM-L1, DSPG3, Dtk, Dynactin subunit 1, Dystroglycan, E-Cadherin, EDA-A2, EDAR, EDIL3, EGF, EGFR, EG-VEGF, ELF3, EMR2, Endocan, Endoglin, Endoglycan, Endorepellin, eNOS, ENPP-2, ENPP-7, Enteropeptidase, Eotaxin-1, Eotaxin-2, Eotaxin-3, EphA1, EphA2, EphB2, EphB3, EphB4, EphB6, Ephrin-A4, Ephrin-B3, Epimorphin, Epiregulin, ER alpha, ErbB2, ErbB3, ErbB4, Erythropoietin, Erythropoietin R, ESAM, E-Selectin, Exostosin-like 3, FABP1, FABP2, FABP3, FABP4, FABP5, FABP6, FABP8, FAM3A, FAM3C, FAP, Fas, Fas Ligand, Fc epsilon RI, Fc gamma RIIB/C, FCAR, FCRL1, FCRL2, FCRL3, FCRL5, FCRLB, Ferritin, Fetuin A, Fetuin B, FGF R3, FGF R5, FGF-12, FGF-16, FGF-17, FGF-19, FGF-20, FGF-21, FGF-23, FGF-3, FGF-4, FGF-5, FGF-6, FGF-7, FGF-9, Fgr, Fibromodulin, Ficolin-1, FKBP51, FKBPR38, FLRG, FLRT1, FLRT2, FLRT3, Flt-3, Flt-3 Ligand, FOLH1, Follistatin, Follistatin-like 1, FOLR1, FOLR2, FOLR3, FosB, FoxM1, FoxO3, FoxP3, Fractalkine, FRS2, FSH, F-Spondin, FUCA1, Fucosyltransferase 8, Furin, FUT11, FXR, Galanin, Galectin-1, Galectin-10, Galectin-2, Galectin-3, Galectin-4, Galectin-7, Galectin-8, Galectin-9, GALNT10, GALNT2, GALNT3, Gas 1, Gas 6, GASP-1, GASP-2, Gastric Intrinsic Factor, Gastrokine-1, GATA-4, GATA-5, GBA3, GCNT1, GCP-2, GCSF, GCSF R, GDF-11, GDF-15, GDF-3, GDF-8, GDF-9, GDNF, GFAP, GFR alpha-1, GFR alpha-2, GFR alpha-3, GITR, GITR Ligand, GLB1, GLI-3, GLP-1, Glucokinase, Glyoxalase 2, Glypican 1, Glypican 2, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, GMF-beta, GOLM1, gp130, GPR111, GPR115, GPR56, GPV, GPVI, Granulysin, Granzyme A, Granzyme B, Granzyme H, GRAP2, GRK5, GRO alpha, GRO alpha/beta/gamma, Growth Hormone, Growth Hormone R, GSK-3 beta, GSTM1, GSTP1, HAI-1, HAI-2, HAPLN1, HAPLN4, HB-EGF, HCC-1, HCC-4, hCG beta, hCG intact, HE4, HepaCAM, Hepassocin, Hepsin, HGF, HGFR, HIF-1 alpha, HMGB3, HNMT, HS3ST1, HS3ST3B1, HS3ST4, HSP32, HTRA2, Human Amyloid-like protein 1, HVEM, Hydroxyacid oxidase 1, I-309, ICAM-1, ICAM-2, ICAM-3, ICOS, IDO, Iduronate 2-Sulfatase, IFN-alpha/beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGF-1, IGF-1 R, IGF-2, IGF-2 R, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, IGFBP-6, IGSF3, IGSF4B, IkB-alpha, IkB-epsilon, IKK-gamma, IL-1 alpha, IL-1 beta, IL-1 R1, IL-1 R2, IL-1 R3, IL-1 R4, IL-1 R6, IL-1 Ra, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17 RA, IL-17 RB, IL-17 RC, IL-17 RD, IL-17A, IL-17B, IL-17C, IL-17E, IL-17F, IL-18, IL-18 BP alpha, IL-18 R alpha, IL-18 R beta, IL-19, IL-2, IL-2 R alpha, IL-2 R beta, IL-2 R gamma, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 R alpha 1, IL-22BP, IL-23, IL-23 R, IL-24, IL-27, IL-27 R alpha, IL-28 R alpha, IL-28A, IL-29, IL-3, IL-31, IL-31 RA, IL-32 alpha, IL-33, IL-34, IL-36 alpha, IL-36 beta, IL-36 gamma, IL-36 Ra, IL-37, IL-38, IL-4, IL-4 R alpha, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9, ILKAP, ILT-2, ILT-4, Inhibin A, Insulin, Insulin R, Integrin alpha-1, Integrin alpha-2, Integrin alpha-4, Integrin alpha-5, Integrin alpha-M, IP-10, IRF-2, IRF-4, ISLR-2, I-TAC, Jagged 1, Jagged 2, JAM-A, JAM-B, JAM-C, JNK2, JunB, Kallikrein 1, Kallikrein 11, Kallikrein 12, Kallikrein 14, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 8, Kell, KIR2DL3, KIR3DL1, KIRREL3, KLF4, Klotho beta, Kremen-2, Kynureninase, LAG-3, LAIR1, LAIR2, Laminin alpha 4, LAMP, LAMP1, LAMP2, Langerin, LAP/TGF beta 1, Latexin, Layilin, LCK, LDL R, LEDGF, Legumain, LEKTI, Leptin, Leptin R, LIF, LIF R alpha, LIGHT, LILRA5, LILRB4, LIMPII, Lin-28A, Lipocalin-1, Lipocalin-2, LMW-PTP, LOX-1, LOXL2, LRIG1, LRIG3, LRP-1 Cluster III, LRP-6, LRPAP, LRRC4, LRRTM1, LRRTM3, LRRTM4, LSECtin, L-Selectin, Lumican, Luteinizing hormone, Lymphotactin, Lymphotoxin beta R, LYVE-1, MAGP-2, MAP1D, Marapsin, Matrilin-2, Matrilin-3, Matriptase, MBL, MCAM, MCEMP1, Mcl-1, MCP-1, MCP-2, MCP-3, MCP-4, M-CSF, M-CSF R, MDC, MDGA1, MDGA2, MDL-1, MDM2, MEF2C, Melan-A, MEPE, Meprin alpha, Meprin beta, Mer, Mesothelin, Mesothelin, METAP2, METTL11A, MFRP, mGluR3, MIA, MICA, MICB, Midkine, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MIP-3 beta, MIS RII, MKK6, MMP-1, MMP-10, MMP-12, MMP-13, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMR, MOG, MPIF-1, MSP alpha/beta, MSP Receptor, NAP-2, N-Cadherin, NCAM-1, NCK1, Nectin-1, Nectin-2, Nectin-3, Nectin-4, NELL1, Neprilysin, Neprilysin-2, Nesfatin-1, Nestin, Netrin-4, Neudesin, Neurexin-3 beta, Neurocan, Neuroglycan C, Neurogranin, Neuroligin 2, Neuropilin-1, Neuropilin-2, Neurturin, NFKB1, NG2, NGFR, NgR3, Nicastrin, Nidogen-1, Nidogen-2, NKp30, NKp44, NKp46, NKp80, Nogo Receptor, Nogo-A, Norrin, Notch-1, Notch-3, NOV, NPDC-1, NPTXR, NQO-1, Nr-CAM, NRF2, NRG1-alpha, NRG1-beta 1, NSE, NT-3, NT-4, NTAL, NTB-A, NTH1, NUDT5, Numb, NUP85, OBCAM, Olfactomedin-2, Olig2, Oligo1, Oligo3, Omgp, Oncostatin M, Oncostatin M R beta, OSCAR, Osteoactivin, Osteoadherin, Osteopontin, Osteoprotegerin, OX40, OX40 Ligand, p130Cas, p27/Kip1, p53, p63, PAG1, PAI-1, PAM, Pancreatic Polypeptide, PAPP-A, PAPP-A Heterotetramic, PAPP-A2, PAR1, PARC, Park7, Pax3, Pax-4, Paxillin, P-Cadherin, PCK1, PCSK2, PD-1, PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PD-L2, PDX-1, PEAR1, PECAM-1, Pentraxin-3, Pepsinogen 1, Pepsinogen 2, Periostin, Peroxiredoxin-1, Peroxiredoxin-2, Peroxiredoxin-4, Persephin, PGCD6, PGRP-S, PI3-Kinase p55-gamma, PILR-alpha, PLA2G4A, Platelet Factor 4, Plexin A4, Plexin B3, Plexin D1, PLGF, PLGF-2, PLUNC, Podocalyxin, Podoplanin, POGLUT1, PON1, PRCP, Pref-1, PRELP, Presenilin 1, Procalcitonin, Pro-EGF, Progranulin, ProGRP, Prolactin, Prolactin R, Proprotein Convertase 9, Prostasin, Proteinase 3, Protocadherin-17, PSA-free, PSA-total, P-Selectin, PSMA1, PSP94, PTH, PTH1R, PTP1B, PTP-MEG2, PU.1, PYY, RAGE, RalA, RalB, RANK, RANTES, RBP4, RCOR1, Reg1B, Reg3A, Reg4, Relaxin-1, RELT, Renin, Resistin, Ret, RGM-A, RGM-B, RGM-C, ROBO2, ROBO3, ROBO4, ROR1, ROR2, R-Spondin 2, R-Spondin 3, Ryk, S100 A1, S100 A13, S100 A2, S100 A6, S100 A8, S100 B, SAA, SALM4, SCCA2, SCF, SCF R, SDF-1 alpha, SDF-1 beta, Semaphorin 4C, Semaphorin 4D, Semaphorin 4G, Semaphorin 6A, Semaphorin 6B, Semaphorin 6C, Semaphorin 6D, Semaphorin 7A, Semaphorin-3E, Serpin A4, Serpin A5, Serpin B2, Serpin B6, Serpin B8, Serpin F1, Serpin A12, SERPINB3, sFRP-3, SH2B1, SH2D1A, SHP-1, SIGIRR, Siglec-1, Siglec-10, Siglec-11, Siglec-2, Siglec-3, Siglec-5, Siglec-6, Siglec-7, Siglec-9, SIRP alpha, SIRP-gamma, Sirtuin 1, Sirtuin 2, Sirtuin 3, Sirtuin 5, SLAM, SLITRK4, SLITRK5, SLPI, SMAD4, SMPD1, SNCG, SOD1, SOD3, Sonic Hedgehog N-Terminal, SorCS1, SorCS2, SorCS3, Sortilin, SOST, SOX10, SOX15, SOX2, SOX7, SOX9, SPARCL1, SP-D, SPHK1, Spinesin, SPINK1, SR-AI, SREC-I, SREC-II, ST3GAL1, ST6GAL1, ST8SIA1, ST8SIA2, Stabilin-2, SULT1B1, SULT1C2, SULT2A1, SULT2B1, Syndecan-1, Syndecan-3, Syndecan-4, Syntaxin 4, Syntaxin 6, TACE, TACI, TAFA1, TAFA2, TAFA5, TARC, TAZ, TBX2, TC-PTP, TECK, Tenascin C, Tenascin R, Testican 1, Testican 2, TFF3, TFPI, TFPI-2, TGF alpha, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta R3, THAP11, Thioredoxin-1, Thrombomodulin, Thrombopoietin, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thrombospondin-5, THSD1, Thyroglobulin, Thyroid Peroxidase, Tie-1, Tie-2, TIGIT, TIM-1, TIM-3, TIM-4, TIMP-1, TIMP-2, TIMP-4, TLR1, TLR2, TLR3, TLR4, TMEFF1, TNF alpha, TNF beta, TNF RI, TNF RII, TPST2, TRACP, TRAF-3 Isoform 2, TRAIL, TRAIL R1, TRAIL R2, TRAIL R3, TRAIL R4, TRANCE, Transcobalamin II, Transferrin, Transglutaminase 3, Transglutaminase 4, Trappin-2, TREM-1, TREM-2, TREML1, Tripeptidyl-peptidase I, TrkA, TRKC, TROP1, TROP-2, Troponin C, Troponin I, TROY, TRP14, Trypsin 1, Trypsin 3, Trypsin Pan, TSC1, TSH, TSLP, TSLP R, Tsukushi, TWEAK, TWEAK R, Ubiquitin+1, UCH-L3, ULBP-1, ULBP-2, ULBP-3, ULBP-4, UNC5H3, UNC5H4, uPA, uPAR, Uromodulin, USAG-1, VAMP-1, VAMP-2, VAP-A, VAP-B, VCAM-1, VE-Cadherin, VEGF-A, VEGF-C, VEGF-D, VEGFR1, VEGFR2, VEGFR3, Visfatin, VSIG4, vWF-A2, WIF-1, WISP-1, Wnt-4, XEDAR, XIAP, ZAP70

Application Notes

Suggested Application

Multiplexed Protein Detection
Detection of Relative Protein Expression
Detecting Patterns of Cytokine Expression
Biomarker Screening
Identifying Key Factors
Confirming a Biological Process

Kit Components

Antibody Array Slide/s
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
30 ml Centrifuge Tube
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with samples
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.