Human Cytokine Array C1

Name: Human Cytokine Array C1
Brand: AAH-CYT-1
SKU: AAH-CYT-1
Price: 429 USD
Availability: InStock
Rating: 100 (2 reviews)

Rating:

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2 Reviews

63 Citations

RayBio^® C-Series Human Cytokine Antibody Array 1 Kit. Detects 23 Humans Cytokines. Suitable for all liquid sample types.

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As low as $429.00

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Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Species	Human
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	23
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	Membrane
Method Of Detection	Chemiluminescence
Design Principle	Sandwich-based
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	-20°C

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Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
GCSF Gene Symbol: CSF3 C17orf33 GCSF Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GM-CSF Gene Symbol: CSF2 GMCSF Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	GRO alpha/beta/gamma Accession #: P09341/P19875/P19876 ELISA Kit: RayBio Human GRO ELISA	GRO alpha (CXCL1) Gene Symbol: CXCL1 GRO GRO1 GROA MGSA SCYB1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A IL1F1 Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA
IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-3 Gene Symbol: IL3 Gene ID: 3562 Accession #: P08700 ELISA Kit: RayBio Human IL-3 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA	IL-6 Gene Symbol: IL6 IFNB2 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA
IL-8 (CXCL8) Gene Symbol: CXCL8 IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA	IL-13 Gene Symbol: IL13 NC30 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA
MCP-1 (CCL2) Gene Symbol: CCL2 MCP1 SCYA2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MCP-2 (CCL8) Gene Symbol: CCL8 MCP2 SCYA10 SCYA8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA	MCP-3 (MARC/CCL7) Gene Symbol: CCL7 MCP3 SCYA6 SCYA7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA	MIG (CXCL9) Gene Symbol: CXCL9 CMK MIG SCYB9 Gene ID: 4283 Accession #: Q07325 ELISA Kit: RayBio Human MIG ELISA	RANTES (CCL5) Gene Symbol: CCL5 D17S136E SCYA5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA
TGF beta 1 Gene Symbol: TGFB1 TGFB Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA TNFB TNFSF1 Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process

Kit Components

Human Cytokine Antibody Array C1 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Other Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Protocol Outline

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.

Documents

Datasheet (AAH-CYT-1.pdf)

SDS (AAH-CYT-1.pdf)

Excel (AAH-CYT-1.xls)

Citations

Desai, Mauli B., et al. "Pollen?induced antigen presentation by mesenchymal stem cells and T cells from allergic rhinitis." Clinical & translational immunology 2.10 (2013).

Species:

Human

Sample Type:

Conditioned Media (PBMCs, with pulsed and unpulsed MSCs)

View Publication

Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007; 6: 1680-1693

Species:

Human

Sample Type:

Conditioned Media (conditioned or non-conditioned medium)

View Publication

Im M., Kim SY., Sohn KC., et al. Epigallocatechin-3-gallate suppresses IGF-I-induced lipogenesis and cytokine expression in SZ95 sebocytes. J Invest Dermatol. 2012 Dec;132(12):2700-8. doi: 10.1038/jid.2012.202.

Species:

Human

Sample Type:

Conditioned Media (SZ95 sebocytes)

View Publication

Norata GD, Tibolla G, Seccomandi PM, Poletti A, Catapano AL. Dihydrotestosterone Decreases Tumor Necrosis Factor-alpha and Lipoploysaccharide-Induced Inflammatory Response in Human Endothelial Cells. J Clin Endocrinol Metab. 2006;91:546-554.

Species:

Human

Sample Type:

Conditioned Media (Human umbilical vein endothelial cell (HUVEC) conditioned media)

View Publication

Zhang R., Jack GS., Rao N., et al. Nuclear fusion-independent smooth muscle differentiation of human adipose-derived stem cells induced by a smooth muscle environment. Stem Cells. 2012 Mar;30(3):481-90. doi: 10.1002/stem.1023.

Species:

Human

Sample Type:

Conditioned Media (Smooth Muscle cells)

View Publication

Joner M, Farb A, Cheng Q, Finn A , Acampado E,et al.Pioglitazone Inhibits In-Stent Restenosis in Atherosclerotic Rabbits by Targeting Transforming Growth Factor- and MCP-1.Arterioscler Thromb Vasc Biol. 2007;27:182

Species:

Human

Sample Type:

Conditioned Media (Cell-conditioned media)

View Publication

Nazzal D., et al. Human thymus medullary epithelial cells promote regulatory T-cell generation by stimulating interleukin-2 production via ICOS ligand. Cell Death and Disease (2014) 5, e1420; doi:10.1038/cddis.2014.377

Species:

Human

Sample Type:

Conditioned Media (mTEC cells)

View Publication

LaValle CR, Zhang L, Xu S, Eiseman JL, Wang QJ. Inducible silencing of protein kinase D3 inhibits secretion of tumor-promoting factors in prostate cancer. Molecular Cancer Therapeutics 2012;11(7):1389-1399. doi:10.1158/1535-7163.MCT-11-0887.

Species:

Human

Sample Type:

Conditioned Media (PC3, prostate cancer cell lines)

View Publication

St Hill, Catherine A., Katherine Krieser, and Mariya Farooqui. "Neutrophil interactions with sialyl Lewis X on human nonsmall cell lung carcinoma cells regulate invasive behavior." Cancer 117.19 (2011): 4493-4505.

Species:

Human

Sample Type:

Conditioned Media

View Publication

Yen Y-T, Liao F, Hsiao C-H, Kao C-L, Chen Y-C, Wu-Hsieh BA. Modeling the Early Events of Severe Acute Respiratory Syndrome Coronavirus Infection In Vitro. J Virol. 2006;80(6):2684-2693.

Species:

Human

Sample Type:

Conditioned Media (THP-1 human monocytic Cell culture supernatant)

View Publication

View All Publications For This Product

Reviews

Write a customer review

Great product

Rating
100%

Straight forward protocol with robust and reproducible results.

Review by Sushil Kumar,

from Dana Farber Cancer Institute,

on Aug 12, 2019
Great product

Rating
100%

It works well. Easy to follow instructions.

Review by Diandra Smith,

from Augusta University,

on Jul 8, 2018

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Featured products

Human Cytokine Array C1

Product Description

Specifications

Risk-Free Guarantee

Amazon Gift Cards!

Product Features

Target Names

Application Notes

Storage/Stability

Documents

Citations

Desai, Mauli B., et al. "Pollen?induced antigen presentation by mesenchymal stem cells and T cells from allergic rhinitis." Clinical & translational immunology 2.10 (2013).

Sze S, Kleijn D, Lai R, Tan E, Zhao H,et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007; 6: 1680-1693

Im M., Kim SY., Sohn KC., et al. Epigallocatechin-3-gallate suppresses IGF-I-induced lipogenesis and cytokine expression in SZ95 sebocytes. J Invest Dermatol. 2012 Dec;132(12):2700-8. doi: 10.1038/jid.2012.202.

Norata GD, Tibolla G, Seccomandi PM, Poletti A, Catapano AL. Dihydrotestosterone Decreases Tumor Necrosis Factor-alpha and Lipoploysaccharide-Induced Inflammatory Response in Human Endothelial Cells. J Clin Endocrinol Metab. 2006;91:546-554.

Zhang R., Jack GS., Rao N., et al. Nuclear fusion-independent smooth muscle differentiation of human adipose-derived stem cells induced by a smooth muscle environment. Stem Cells. 2012 Mar;30(3):481-90. doi: 10.1002/stem.1023.

Joner M, Farb A, Cheng Q, Finn A , Acampado E,et al.Pioglitazone Inhibits In-Stent Restenosis in Atherosclerotic Rabbits by Targeting Transforming Growth Factor- and MCP-1.Arterioscler Thromb Vasc Biol. 2007;27:182

Nazzal D., et al. Human thymus medullary epithelial cells promote regulatory T-cell generation by stimulating interleukin-2 production via ICOS ligand. Cell Death and Disease (2014) 5, e1420; doi:10.1038/cddis.2014.377

LaValle CR, Zhang L, Xu S, Eiseman JL, Wang QJ. Inducible silencing of protein kinase D3 inhibits secretion of tumor-promoting factors in prostate cancer. Molecular Cancer Therapeutics 2012;11(7):1389-1399. doi:10.1158/1535-7163.MCT-11-0887.

St Hill, Catherine A., Katherine Krieser, and Mariya Farooqui. "Neutrophil interactions with sialyl Lewis X on human nonsmall cell lung carcinoma cells regulate invasive behavior." Cancer 117.19 (2011): 4493-4505.

Yen Y-T, Liao F, Hsiao C-H, Kao C-L, Chen Y-C, Wu-Hsieh BA. Modeling the Early Events of Severe Acute Respiratory Syndrome Coronavirus Infection In Vitro. J Virol. 2006;80(6):2684-2693.

Reviews

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