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    Human CXCL14 ELISA Kit

    Rating:
    93 % of 100
    3  Reviews
    3 Citations

    RayBio® Human CXCL14 (BRAK) ELISA Kit for cell culture supernatants, plasma, and serum samples.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

    Documents
    As low as $420.00
    In stock
    Only %1 left
    Catalog #:
    ELH-CXCL14
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human
    Accession Number O95715
    Gene Id 9547
    Gene Symbols CXCL14|MIP2G|NJAC|SCYB14|PSEC0212|UNQ240/PRO273
    Protein Name / Synonyms C-X-C motif chemokine 14 (Chemokine BRAK) (MIP-2G) (Small-inducible cytokine B14)
    Quantitative/Semi-Quantitative Quantitative
    Specificity This ELISA antibody pair detects human CXCL14. Other species not determined.
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Sensitivity 700 pg/mL

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    Detection Range 700 pg/ml - 21000 pg/mL
    Recommended Dilution (Serum/Plasma) 3 fold
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage ≤-20°C

    Risk-Free Guarantee

    We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.

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    Product Features

    Additional Notes
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Citations

    Cereijo R., Gavalda-Navarro A., Cairo M., et al. CXCL14, a Brown Adipokine that Mediates Brown-Fat-to-Macrophage Communication in Thermogenic Adaptation. Cell Metab . 2018 Nov 6;28(5):750-763.e6. doi: 10.1016/j.cmet.2018.07.015. Epub 2018 Aug 16.
    Species:
    Human
    Sample Type:
    Plasma, Conditioned Media (Role of CXCL14 in brown fat)
    View Publication
    García-Beltran C, Cereijo R, Quesada-López T, et al. Reduced circulating levels of chemokine CXCL14 in adolescent girls with polycystic ovary syndrom: normalization after insulin sensitization. BMJ Open Diabetes Research and Care 2020; e001035. doi: 10.1136/bmjdrc-2019-001035
    Species:
    Human
    Sample Type:
    Serum, Conditioned Media
    View Publication
    García-Beltran C, Cereijo R, Quesada-López T, et al. Reduced circulating levels of chemokine CXCL14 in adolescent girls with polycystic ovary syndrome: normalization after insulin sensitization. BMJ Open Diab Res Care 2020;8:e001035. doi:10.1136/ bmjdrc-2019-001035
    Species:
    Human
    Sample Type:
    Serum, Conditioned Media (cell culture media)
    View Publication

    Reviews

    Write a customer review
    1. Great product
      Rating
      80%
      Works well with human urine samples.

      Review by

      from University of Houston,

      on

    2. Great product
      Rating
      100%
      Very easy to use. This is my second time using a RayBiotech ELISA kit. Great customer service as well.

      Review by

      from Meharry Medical College,

      on

    3. Great product
      Rating
      100%
      The level of secreted CXCL14 from conditioned media of human preadipocytes and adipocytes was assessed using this kit. The kit is very sensitive and even though the kit was opened and stored at -20C (unused wells and all other reagents), and used after a year, the sensitivity is still high and does not diminish.

      Review by

      on

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