Human CD47 ELISA Kit

Name: Human CD47 ELISA Kit
Brand: ELH-CD47
SKU: ELH-CD47
Price: 480 USD
Availability: InStock
Rating: 80 (1 reviews)

Rating:

80 % of 100

1 Review (include details such as sample type used, experimental conditions, etc.)

RayBio® Human CD47 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Human
Accession Number	Q08722
Gene Id	961
Gene Symbols	CD47\|MER6
Protein Name / Synonyms	Leukocyte surface antigen CD47 (Antigenic surface determinant protein OA3) (Integrin-associated protein) (IAP) (Protein MER6) (CD antigen CD47)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA antibody pair detects Human CD47. Other species not determined.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Research Area	Antibody Drug Development, Protein Therapeutics, Biomarker Detection
Sensitivity	0.52 ng/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	0.52 ng/ml -125 ng/ml
Recommended Dilution (Serum/Plasma)	4 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELH-CD47.pdf)

SDS (ELH-CD47.pdf)

Excel (sandwich-elisa.xlsm)

Reviews

Write a customer review

Great product

Rating
80%

This assay works very well in both serum and EDTA-plasma matrices with good dilutional linearity and % spike recovery

Review by Anonymous,

from Gilead Sciences,

on Apr 17, 2021

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Human CD47 ELISA Kit