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Human Cancer Biomarker Array 1
Multiplex ELISA array detects 18 human cancer biomarker proteins. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
|---|---|
| Species | Human |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 18 |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates |
| Solid Support | Glass Slide |
| Method Of Detection | Fluorescence Laser Scanner |
| Design Principle | Sandwich-based |
| Research Area | Cancer |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | -20°C |
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$5 Amazon gift card in every kit box purchased.
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Alpha-fetoprotein
| CA125
| CA15-3
| CA19-9
| CA72-4
|
CEA
| Cytokeratin 19 (KRT19)
| GOLM1 (GP73)
| hCG beta
| HE4 (WFDC2)
|
NSE
| Pepsinogen 1
| Pepsinogen 2
| ProGRP
| PSA-free
|
PSA-total
| SERPINB3 (SCCA1)
| Thyroglobulin
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Cancer Biomarker Array Q1 Slide(s)
- Sample Diluent
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- 1.5 ml Polypropylene microcentrifuge tubes
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
