Human beta-NGF ELISA Kit

Name: Human beta-NGF ELISA Kit
Brand: ELH-BNGF
SKU: ELH-BNGF
Price: 420 USD
Availability: InStock
Rating: 100 (5 reviews)

Rating:

100 % of 100

5 Reviews

15 Citations

RayBio® Human beta-NGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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As low as $420.00

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Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Human
Accession Number	P01138
Gene Id	4803
Gene Symbols	NGF\|NGFB
Protein Name / Synonyms	Beta-nerve growth factor (Beta-NGF)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	14 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	14 pg/ml - 5000 pg/ml
Recommended Dilution (Serum/Plasma)	2 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Target Background

Nerve growth factor β (BNGF) is a neurotrophin essential for the development and functional maintenance of neurons in the central (cholinergic) and peripheral nervous system. It is translated as a precursor pro-protein which undergoes cleavage to produce a biologically active fragment, which is then homodimerized and secreted. The main receptor for BNGF is tyrosine kinase receptor A (TRKA). Mutations in the BNGF gene are associated with loss of pain perception (hereditary, sensory and autonomic neuropathy, type 5 (HSAN5)), mental retardation and immune deficiency.

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELH-BNGF.pdf)

SDS (ELH-BNGF.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Sun J., Mandai M., Kamao H., et al. Protective Effects of Human iPS-Derived Retinal Pigmented Epithelial Cells in Comparison with Human Mesenchymal Stromal Cells and Human Neural Stem Cells on the Degenerating Retina in rd1 mice. Stem Cells. 2015 May;33(5):1543-53. doi: 10.1002/stem.1960.

Species:

Human

Sample Type:

Conditioned Media (Induced Pluripotent Stem Cells)

View Publication

Dias, B., SerrÃ£o, P., Cruz, F. et al. Effect of Water Avoidance Stress on serum and urinary NGF levels in rats: diagnostic and therapeutic implications for BPS/IC patients. Sci Rep 9, 14113 (2019). https://doi.org/10.1038/s41598-019-50576-4

Species:

Rat

Sample Type:

Plasma, Urine

View Publication

Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.

Species:

Human

Sample Type:

Conditioned Media (Vertebral disc conditioned media)

View Publication

Krock E., Currie J., Weber M., et al. Nerve Growth Factor Is Regulated by Toll-Like Receptor 2 in Human Intervertebral Discs. J Biol Chem. 2016 Feb 12;291(7):3541-51. doi: 10.1074/jbc.M115.675900

Species:

Human

Sample Type:

Conditioned Media (Nucleus Pulposus and Annulus Fibrosus cells)

View Publication

Faroni A., Smith R., Lu L., et al. Human Schwann-like cells derived from adipose-derived mesenchymal stem cells rapidly de-differentiate in the absence of stimulating medium. Eur J Neurosci. 2016 Feb;43(3):417-30. doi: 10.1111/ejn.13055.

Species:

Human

Sample Type:

Conditioned Media (Subcutaneous abdominal adipose tissue derived stem cells)

View Publication

Reid, Adam J., et al. "Nerve repair with adipose-derived stem cells protects dorsal root ganglia neurons from apoptosis." Neuroscience 199 (2011): 515-522.

Species:

Human

Sample Type:

Conditioned Media (Adipose-derived stem cells or primary Schwann cells)

View Publication

Kalinowska-lyszczarz A., et al. Immune cell NT-3 expression is associated with brain atrophy in multiple sclerosis patients. J Neuroimmunol. 2011 Dec 15;240-241:109-13. doi: 10.1016/j.jneuroim.2011.10.002.

Species:

Human

Sample Type:

Cell Lysate (PBMC)

View Publication

Kalinowska-lyszczarz A., et al. Immune cell NT-3 expression is associated with brain atrophy in multiple sclerosis patients. J Neuroimmunol. 2011 Dec 15;240-241:109-13. doi: 10.1016/j.jneuroim.2011.10.002.

Species:

Human

Sample Type:

Serum

View Publication

Lambiase, Alessandro, Mauro P. Ferrari, and Flavio Mantelli. "Safety and Pharmacokinetics of Escalating Doses of Human Recombinant Nerve Growth Factor Eye Drops in a Double-Masked, Randomized Clinical Trial." BioDrugs. Springer Link, Dec. 2013. Web.

Species:

Human

Sample Type:

Serum (patients received rhNGF and donated blood samples)

View Publication

Portone A., Moffa M., Gardin C., et al. Lineage-Specific Commitment of Stem Cells with Organic and Graphene Oxide-Functionalized Nanofibers. Adv. Funct. Mater. 2018, 1806694

Species:

Human

Sample Type:

Conditioned Media (Dental Pulp stem cells)

View Publication

View All Publications For This Product

Reviews

Write a customer review

Great product

Rating
100%

I love this product. If I didn't have it I would get fired.

Review by Sagan,

from Integral BioSystems, LLC,

on Nov 16, 2022
Great product

Rating
100%

A fan, ordered again!

Review by Victoria,

from University of Northern Colorado,

on Oct 8, 2019
Great product

Rating
100%

Easy-to-use kit. Will order again in future.

Review by Victoria,

from University of Northern Colorado,

on Sep 27, 2019
Great product

Rating
100%

The product was user friendly. As a first time student conducting an ELISA, the directions were workable. The direction paper has everything you need, plus example dilutions. The standards were also easy to understand. Will get this kit again when I need to analyze NGF!

Review by Vi,

from University of Northern Colorado,

on Jan 26, 2019
Great product

Rating
100%

This kit worked great for detecting NGF in human serum and plasma samples. Like all their other kits it was very easy to use and straight forward. The suggested dilution of the kit is 2:1 for human samples. However, I suggest, if you have sample to spare, to run the serum or plasma straight to avoid falling near the bottom of the standard curve.

Review by Jonathon Lisano,

from University of Northern Colorado,

on Nov 4, 2018

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Human beta-NGF ELISA Kit