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    ANP ELISA Kit

    Rating:
    87 % of 100
    3  Reviews
    9 Citations
    Human/Mouse/Rat Atrial Natriuretic Peptide EIA Kit optimized for serum and cell culture medium. Competition-based ELISA on a 96-well strip plate.
    Documents
    As low as $397.00
    In stock
    Only %1 left
    Catalog #:
    EIA-ANP
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human, Mouse, Rat
    Accession Number P01160
    Gene Id 4878
    Gene Symbols NPPA|ANP|PND
    Protein Name / Synonyms Natriuretic peptides A (CDD-ANF) (Prepronatriodilatin) [Cleaved into: Cardiodilatin-related peptide (CDP) Atrial natriuretic factor (ANF) (Atrial natriuretic peptide) (ANP)]
    Quantitative/Semi-Quantitative Quantitative
    Specificity This kit targets the common sequence of human, mouse and rat, and thus may be used to detect ANP expression in all these species with high specificity and sensitivity.

    Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.
    Compatible Sample Types Cell Culture Supernatants, Serum
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Competition-based
    Sensitivity 1.02 pg/ml
    Detection Range 0.1-1,000 pg/ml
    Recommended Dilution (Serum/Plasma) Human: 4X / Mouse: 4X / Rat: 4X
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage -20°C

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    Introduction

    Atrial natriuretic peptide (ANP) is a 28-amino acid peptide hormone secreted by cardiac myocytes of the atrium. ANP plays an important role in the homeostatic regulation of body water, sodium, potassium and fat, by acting to reduce the water, sodium and adipose loads on the circulatory system, thus reducing blood pressure.

    ANP peptide contains a 17-amino acid ring which is formed by a disulfide bond between two cysteine residues at positions 7 and 23. ANP is closely related to BNP (brain natriuretic peptide) and CNP (C-type natriuretic peptide), which all share the same amino acid ring.

    The mechanism of ANP-induced vasodilatation is through binding to a specific set of ANP receptors. Receptor-agonist binding causes a reduction in blood volume and therefore a reduction in cardiac output and systemic blood pressure. Lipolysis is increased and renal sodium reabsorption is decreased. The overall effect of ANP on the body is to counter increases in blood pressure and volume caused by the renin-angiotensin system.

    In addition to its vasodilatation effect, ANP also serves as a adipokine. Studies have shown that ANP Increases the release of free fatty acids from adipose tissue, activates adipocyte plasma membrane NPR-A receptors, and increases intracellular cGMP levels that induce the phosphorylation of a hormone-sensitive lipase and perilipin A.

    Product Features

    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 ml volumes
    • Adjustable 1-25 ml pipettes for reagent preparation
    • 100 ml and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
    Protocol Outline
  • Prepare all reagents, samples and standards as instructed.
  • Add 100 µl detection antibody to each well.
  • Incubate 1.5 h at RT or O/N at 4°C.
  • Add 100 µl standard or sample to each well.
  • Incubate 2.5 h at RT.
  • Add 100 µl prepared streptavidin solution.
  • Incubate 45 min at RT.
  • Add 100 µl TMB One-Step Substrate Reagent to each well.
  • Incubate 30 min at RT.
  • Add 50 µl Stop Solution to each well.
  • Read plate at 450 nm immediately.

    • Storage/Stability

    Standard, Biotinylated Atrial natriuretic peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

    Citations

    Muchir A., et al. Treatment with selumetinib preserves cardiac function and improves survival in cardiomyopathy caused by mutation in the lamin A/C gene. Cardiovasc Res. 2012 Feb 1;93(2):311-9. doi: 10.1093/cvr/cvr301
    Species:
    Mouse
    Sample Type:
    Serum
    View Publication
    Hamdani N., et al. Left ventricular diastolic dysfunction and myocardial stiffness in diabeticmice is attenuated by inhibition of dipeptidyl peptiase 4. Cardiovascular Research (2014) 104, 423-431. doi:10.1093/cvr/cvu223
    Species:
    Mouse
    Sample Type:
    Serum (Sitagliptin, DPP-4 inhibitor)
    View Publication
    Egom E., Vella K., Hua R., et al. Impaired sinoatrial node function and increased susceptibility to atrial fibrillation in mice lacking natriuretic peptide receptor C. J Physiol 593.5 (2015) pp 1127-1146
    Species:
    Mouse
    Sample Type:
    Plasma (Cardiac studies)
    View Publication
    Asmar A., Simonsen L., Asmar M., et al. Renal extraction and acute effects of glucagon-like peptide-1 on central and renal hemodynamics in healthy men. Am J Physiol Endocrinol Metab. 2015 Apr 15;308(8):E641-9. doi: 10.1152/ajpendo.00429.2014.
    Species:
    Human
    Sample Type:
    Plasma
    View Publication
    Kim M, Platt MJ, Shibasaki T, Quaggin SE, Backx PH, et al. GLP-1 receptor activation and Epac2 link atrial natriuretic peptide secretion to control of blood pressure. Nat Med. 2013;19:567-575.
    Species:
    Mouse
    Sample Type:
    Serum (Serum and cell culture medium)
    View Publication
    Ku HC., et al. DPP4 deficiency preserves cardiac function via GLP-1 signaling in rats subjected to myocardial ischemia/reperfusion. Naunyn Schmiedebergs Arch Pharmacol. 2011 Aug;384(2):197-207. doi: 10.1007/s00210-011-0665-3.
    Species:
    Rat
    Sample Type:
    Plasma
    View Publication
    Staedtke V., Bai R., Kim K., et al. Disruption of a self-amplifying catecholamine loop reduces cytokine release syndrome. Nature. 2018 Dec;564(7735):273-277. doi: 10.1038/s41586-018-0774-y
    Species:
    Mouse
    Sample Type:
    Conditioned Media (C nogyi NT treated cells)
    View Publication
    Samillan V., et al. Combination of erythropoietin and sildenafil can effectively attenuate hypoxia-induced pulmonary hypertension in mice. Pulm Circ. 2013 Dec;3(4):898-907. doi: 10.1086/674758
    Species:
    Mouse
    Sample Type:
    Serum
    View Publication
    Staedtke V., Bai R., Kim K., et al. Disruption of a self-amplifying catecholamine loop reduces cytokine release syndrome. Nature. 2018 Dec;564(7735):273-277. doi: 10.1038/s41586-018-0774-y
    Species:
    Mouse
    Sample Type:
    Plasma (C nogyi NT treated cells)
    View Publication

    Reviews

    Write a customer review
    1. Great product
      Rating
      100%
      Great kit, easy to use, highly recommend

      Review by

      from University at Buffalo,

      on

    2. Great product
      Rating
      80%
      The kit worked well for detecting human ANP. The instructions are clear, the manual is well written and easy to follow. We used the recommended 4x dilution of the samples and the sensitivity was on the lower end, but still detected ANP from serum.

      Review by

      from Augusta University,

      on

    3. Great product
      Rating
      80%
      Well explained method. High sensitivity. Worked very well with human and mouse samples. Highly recommend this kit.

      Review by

      from University of Buffalo,

      on

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