RayBio® Human ANGPTL4 ELISA Kit for cell culture supernatants, plasma, and serum samples.

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Catalog #:
ELH-ANGPTL4
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species Human
Accession Number Q9BY76
Gene Id 51129
Gene Symbols ANGPTL4|ARP4|HFARP|PGAR|PP1158|PSEC0166|UNQ171/PRO197
Protein Name / Synonyms Angiopoietin-related protein 4 (Angiopoietin-like protein 4) (Hepatic fibrinogen/angiopoietin-related protein) (HFARP)
Quantitative/Semi-Quantitative Quantitative
Specificity This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF
Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Research Area Angiogenesis
Sensitivity 20 pg/ml

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Detection Range 20 pg/ml - 20000 pg/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage ≤-20°C

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Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Clasen BFF, Krusenstjerna-Hafstrom T, Vendelbo MH, et al. Gene Expression in Skeletal Muscle after an Acute Intravenous GH Bolus in Human Subjects: Identification of a Mechanism Regulating ANGPTL4. J Lipid Res. 2013;57(7):1988-1997.
Species:
Human
Sample Type:
Serum (healthy subjects vs. subjects with GH deficiency)
Drager LF, Yao Q, Hernandez KL, et al. Chronic Intermittent Hypoxia Induces Atherosclerosis via Activation of Adipose Angiopoietin-like 4. Am. J. Respir. Crit. Care Med. 2013 Jan 17;
Species:
Human
Sample Type:
Serum (cited in supplemental data & methods)
Basak S, Duttaroy AK. cis-9,trans-11 conjugated linoleic acid stimulates expression of angiopoietin like-4 in the placental extravillous trophoblast cells. Biochim Biophys Acta. 2013;1831(4):834-843.
Species:
Human
Sample Type:
Conditioned Media (HTR8 cells)
Basak, Sanjay, and Asim K. Duttaroy. "Effects of fatty acids on angiogenic activity in the placental extravillious trophoblast cells." Prostaglandins, Leukotrienes and Essential Fatty Acids 88.2 (2013): 155-162.
Species:
Human
Sample Type:
Conditioned Media (HTR8 cells - fatty acid effects)
Baranowski, T., et al. "Serum levels of the adipokine fasting-induced adipose factor/angiopoietin-like protein 4 depend on renal function." Hormone and metabolic research 43.02 (2011): 117-120.
Species:
Human
Sample Type:
Serum
Angelini PD, Zacarias Fluck MF, Pederson K, et al. Constitutive HER2 signaling promotes breast cancer metastasis through cellular senescence. Cancer Res. 2013;73(1); 450-458.
Species:
Human
Sample Type:
Conditioned Media (MCF7 (breast cancer) cell line, treated vs. untreated)
Angelini PD, Zacarias Fluck MF, Pederson K, et al. Constitutive HER2 signaling promotes breast cancer metastasis through cellular senescence. Cancer Res. 2013;73(1); 450-458.
Species:
Human
Sample Type:
Serum
Wadood S., Saihood Y., Mohammed Z. Determination of Angiopoietin Like Protein-4 Levels and Some Parameters in Iraqi Patients with Type 2 Diabetes Mellitus. Journal of Al-Nahrain University, Vol.18 (2), June, 2015, pp.33-43
Species:
Human
Sample Type:
Plasma (Type 2 Diabetes Iraqi patients)
Wang, Ying, et al. "Severity of diabetes governs vascular lipoprotein lipase by affecting enzyme dimerization and disassembly." Diabetes 60.8 (2011): 2041-2050.
Species:
Rat
Sample Type:
Serum
Adhikary, Till, et al. "Inverse PPAR?/? agonists suppress oncogenic signaling to the ANGPTL4 gene and inhibit cancer cell invasion." Oncogene 32.44 (2013): 5241.
Species:
Human
Sample Type:
Conditioned Media (MDA-MB-231 cells)
  1. Great Kit
    Rating
    100%
    Easy to detected the cell culture supernatant samples as well as blood samples. Works well.

    Review by

    from Emory University,

    on

  2. Great product
    Rating
    80%
    Standards worked fine. Urine samples without dilution are good for this marker

    Review by

    from University of Houston,

    on

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