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    Hippo Pathway Screening Array

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    Screen the relative levels of 118 human proteins involved in Hippo signaling pathway. Suitable for serum, plasma, cell culture supernatants, and lysates.

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    As low as $495.00
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    Catalog #:
    AAH-BLG-HIP
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    Product Description

    Specifications

    Size 4 Sample Kit, 8 Sample Kit
    Species Human
    Quantitative/Semi-Quantitative Semi-Quantitative
    Number of Targets Detected 118
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
    Solid Support Glass Slide
    Method Of Detection Fluorescence Laser Scanner
    Design Principle Label-based
    Research Area Hippo Signaling
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage -20°C
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    $5 Amazon gift card in every kit box purchased.

    Product Features

    • High density arrays
    • Unbiased detection through direct biotin labeling of samples
    • Low sample consumption
    • High detection sensitivity
    • Same day result
    • Affordable and simple to use

    Target Names

    14-3-3 beta/alpha, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 theta, 14-3-3 zeta/delta, ACTG1, aFGF / FGF-1, AJUBA, ALK-6 / BMPR-IB, Alpha-fetoprotein, AMH, Amphiregulin, APC, AXIN1, AXIN2, BMP-2, BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-8B / OP-2, BMPR-IA / ALK-3, BMPR-II, BTRC, CCN2, CCND2, CCND3, cIAP-1 / BIRC2 / hIAP-2, cIAP-2, c-Myc / MYC, CSNK1D, CSNK1E, CTNNA3, CTNNB1, Cyclin D1, DLG4, DVL1, DVL2, DVL3, E-Cadherin, FBXW11, Frizzled-1, Frizzled-2, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Frizzled-8, GDF5, GDF-7 / BMP-12, GDF-8 / Myostatin, GLI2, GSK-3 beta, ID1, ID2, ITGB2, LEF1, LIMD1, MOB1B, MPP5, NF2, PAK1, PARD3, PARD6A, PARD6B, PPP1CA, PPP1CC, PPP2CA, PPP2CB, PPP2R1A, PPP2R1B, PPP2R2A, PPP2R2B, PPP2R2C, PRKCI, PRKCZ, RASSF1, RASSF2, Serpin E1, SMAD1, SMAD2, SMAD3, SMAD4, SMAD7, SOX2, STK3, Survivin / BIRC5, TAZ / WWTR1, TCF7, TCF7L1, TGF beta 1, TGF beta 2, TGF beta 3, TGF beta R1 / ALK 5, TGF beta R2, TP73, WNT1, Wnt-10a, Wnt-10b, Wnt-11, Wnt-16, Wnt-2, Wnt-2b, Wnt-3a, Wnt-4, Wnt5a, Wnt-5b, Wnt-6, Wnt-7a, Wnt-7b, Wnt-8b, Wnt-9a, Wnt-9b, WWC1, YAP1

    Application Notes

    Suggested Application
    Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
    Kit Components
    • RayBio® L-Series Antibody Array Glass Slide(s)
    • Spin Columns
    • Labeling Reagent
    • Stop Solution
    • Blocking Buffer
    • Wash Buffer 1
    • Wash Buffer 2
    • Streptavidin-Conjugated Fluor
    • Adhesive Plastic Strips
    • Labeling Buffer
    • Lysis Buffer
    • 30 ml Centrifuge Tube
    Other Materials Required
    • Distilled or de-ionized water
    • Small plastic or glass containers
    • Orbital shaker or oscillating rocker
    • 1 ml tube
    • Pipettors, pipette tips and other common lab consumables
    • Aluminum foil
    • Gene microarray scanner or similar laser fluorescence scanner

    View Compatible Laser Scanners

    Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

    Protocol Outline
    1. Purify Samples with Spin Column
    2. Biotinylate Samples
    3. Purify biotinylated samples using spin column
    4. Dry the glass slide
    5. Block array surface
    6. Incubate with Samples
    7. Incubate with Streptavidin-Conjugated Fluor
    8. Disassemble the glass slide
    9. Scan with a gene microarray laser scanner
    10. Perform densitometry and analysis

    Storage/Stability

    For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

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