Universal Nuclease (DNase / RNase)

Recombinant universal nuclease affinity-purified from E. coli with high endonuclease activity. Tolerates high concentrations of urea, which is commonly used for insoluble protein solubilization and purifications. Degrades all forms of DNA and RNA.

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$99.00

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Product Description

Specifications

Size	10 KU (400 µl)
Species	Serratia marcescens
Gene Symbols	nucA
Protein Name / Synonyms	Nuclease, endonuclease, unrestricted endonuclease, Dnase / RNase
Expression System	E. coli
Tag	C-terminal His-tag
Purification	His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity	>95%
Purity Determined By	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)	27 kDa
Format	Liquid
Formulation	Filtered solution in 20 mM Tris-HCl (pH 8.0), 50 mM NaCl, 5 mM MgCl2, 50% glycerol.
Concentration (lot specific)	25 units/µl
Activity	25 U/µl. One unit is the cleavage of 37 µg salmon sperm DNA (ssDNA) at 37°C in 30 minutes. Product is compatible with the diversity condtions: buffer pH 7-10, temperature (4-40°C), 0-4 M urea (See Fig. 2), etc.
Endotoxin Level	< 0.5 EU per µg of the protein as determined by the LAL method
Unit Definition	One unit cleaves 37 µg salmon sperm DNA (ssDNA) at 37°C in 30 minutes.
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	-20°C

Images

Quality control image — **Fig. 1 SDS-PAGE analysis of purified product. M, protein ladder (kDa).**

DNA cleavage assay competitor comparison — **Fig. 2 DNA cleavage assay**

**Fig. 3 Universal Nuclease digestion reduce sample viscosity.**

Instructions

Resuspend the cell pellets in any physiological buffers at the appropriate ratio, for example, 10~20/1 (V/W, 10-20 ml buffer/1 g wet cells). Typically for bacteria, we use 20 mM Tris-HCl (pH 8), 2.5 mM MgCl₂, 50 mM NaCl.
Add appropriate Universal Nuclease to the suspension (normally adding 250 U enzymes/1 g wet cell paste)
Break the cells through ultrasonication, tissue homogenizer, freeze-thaw cycles, high pressure homogenizer, etc.
Clarify the cell lysate by high speed centrifugation at 20,000 g for 30 minutes at 4°C.
Transfer the clear supernatants to new tubes for downstream work.

Stability & Storage

Shipping

The product is shipped with ice packs. Upon arrival, immediately store it at the temperature recommended below. It is also supplied with one vial of 10x Cleavage Buffer (200 mM Tris-HCl, 25 mM MgCl2, 500 mM NaCl, pH 8.0), 1 mL.

Storage/Stability

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. The shelf life is up to 6 months from the date of receipt at -20°C or -80°C under sterile condition.

Documents

Datasheet (230-06001.pdf)

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Universal Nuclease (DNase / RNase)