Albumin is a 66.5 kDa globular plasma protein synthesized by the liver and rapidly excreted into the bloodstream. The main function of albumin is to regulate the osmotic pressure of blood. A patient's liver function can be measured by checking their serum albumin levels. Albumin levels can be affected by a poor nutritional state or liver/kidney dysfunction. Low albumin levels can be a sign of liver or kidney disease.
Product Features
Additional Notes
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
This kit works very well for urine samples. I used a sample dilution of 1:250.
Review byRyan,
fromUniversity of Houston,
on
Great product
Rating
100%
The kit is easy to use. The instructions are straight-forward, easy to follow, and produce reliable, replicable results. With its relatively short incubation times, this kit is a great product.
Review byJason Albert,
fromUniversity of Michigan,
on
Great product
Rating
100%
The ELISA kit for Human Albumin is simply fantastic and easy to use. The procedure is very quick and gives you great and reliable results. Super recommended.
