Rapid Isothermal PCR Assay Development

RT-LAMP - This reaction is unique in that you only require a hot block that is capable of holding temperatures between 50-70 degrees C.

qPCR is excellent for most research projects with "normal" samples that don't have known PCR interferants, and especially with purified samples. However, RT-LAMP can be more sensitive in these nonpurified samples, and also doesn't suffer from the same interfering components as PCR is. Additionally, another consideration is the shear equipment requirements, with LAMP needing only a basic hot block and not a more sophisticated thermocycler. But both are great assays, and the choice is really customer need dependent.

RT-LAMP is not suitable for high-throughput applications, as it is difficult to scale up the reaction. It is also more susceptible to contamination than PCR-based methods, especially in samples that are non-purified.

PCR is susceptible to contamination, especially in unpurified samples, as numerous compounds are known to interfere with the polymerase or other steps in the reaction.