The RayBio® COVID-19 Spike-ACE2 binding assay kit is a patented, rapid, simple, and sensitive method to characterize the binding affinity of the SARS-CoV-2 Spike (S) protein and the Angiotensin I Converting Enzyme 2 (ACE2) receptor complex in the presence of potential inhibitors.
Positive and negative controls for the Spike-ACE2 binding assays are available as an accessory item. Click here.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Human, SARS-CoV-2 |
| Accession Number | QHD43416 |
| Gene Symbols | ACE2|S|RBD|SRBD|S-RBD |
| Protein Name / Synonyms | Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, ACEH, Metalloprotease MPROT15, Processed angiotensin-converting enzyme 2, ACE2, Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD) |
| Format | ACE2 on plate + Spike RBD query |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Compatible Sample Types | Plasma, Serum, Antibodies, Proteins, Small Molecules, Peptides |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Research Area | Protein Therapeutics, Receptor-Ligand Binding |
| Estimated Lead Time | 3-5 business days |
| Shipping Type | Blue ice |
| Storage | 4°C |
| Note | The binding assay comes in two different options based on which protein is immobilized on the plate and which protein is added in solution (i.e. "query"). It is the researcher's responsibility to select the format for their study. |
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Pirovano, G., Ordonez, A. A., Jain, S. K., Reiner, T., Carroll, L. S., & Pillarsetty, N. V. K. (2021). Rapid detection of SARS-CoV-2 using a radiolabeled antibody. Nuclear Medicine and Biology, 98-99, 69-75. doi:10.1016/j.nucmedbio.2021.05.1
Tsukinoki, Keiichi et al. "Detection of cross-reactive IgA against SARS-CoV-2 spike 1 subunit in saliva." medRxiv 2021.03.29.21253174; doi: https://doi.org/10.1101/2021.03.29.21253174
Boroduske, Anete et al. "Wild Sambucus nigra L. from north-east edge of the species range: A valuable germplasm with inhibitory capacity against SARS-CoV2 S-protein RBD and hACE2 binding in vitro." Industrial crops and products vol. 165 (2021): 113438. doi:10.1016/j.indcrop.2021.113438
Olaleye, Omonike A et al. "Discovery of Clioquinol and analogues as novel inhibitors of Severe Acute Respiratory Syndrome Coronavirus 2 infection, ACE2 and ACE2 - Spike protein interaction in vitro." Heliyon vol. 7,3 (2021): e06426. doi:10.1016/j.heliyon.2021.e06426
Olaleye, Omonike A et al. "Ambroxol Hydrochloride Inhibits the Interaction between Severe Acute Respiratory Syndrome Coronavirus 2 Spike Protein's Receptor Binding Domain and Recombinant Human ACE2." bioRxiv 2020.09.13.295691; doi: https://doi.org/10.1101/2020.09.13.295691
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Fantastic KitOverall this kit was very easy to use and offered reliable, replicable results. The modular test strips made for easy testing setups without having to commit to running a full 96 wells, which allowed us to do easy preliminary testing while still getting used to the kit. Our only complaint is that it doesn't last longer. Would love to see the development of a more stable kit.
Overall this kit perfectly suited our needs and we will most definitely be ordering more in the future.on
Great productWe followed the exact protocol on from the manual. The kit is easy to use. Gives rapid results.from Leveragen, Inc.,
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Great productThe kit is easy to use and reliable. We have generated lots of data using this kit. I like this particular kit (CoV-ACE2S2-1) because the RBD is Fc-Tagged allowing to test the effects of diverse agents even spike proteins.from Georgetown University,
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Great productPositive feed back from our R&D team.from AIVITA Biomedical,
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Great productThe kit is great, we have use 3 kits already. Something extra to say not product related itself, is BE CAREFUL with the temperature labeled in the shipping box. All items I've got from RayBiotech has came with a -20C storage recommendation when the actual kit temp is 4C and few items are -20. My coworker didnt check and all my kit got into -20 overnight until I came next day and verify it.from Stanford University,
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Great productKit doesn't come with kind of protocol but, works very well.from The University of Cincinnati,
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Great productSome of the wells did work well. Next time we are planning to do an overnight incubation to give it a better chance. I'm ordering a 2nd kit.from Stanford University,
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Great productwe tested the transduction efficiency of the viruses on wild type 293 cells... plasma samples were used as negative.from NANOBST,
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Great productI checked inhibition of SARS-CoV2 spike protein and ACE2 interaction by using using our developed vaccine and it worked really well.from Washington University in St. Louis,
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Great productKit helped us to check whether the our test compound is inhibiting the interaction or notfrom South dakota state university,
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Great productPositive control along with kit showed a good signal. However, our RBD antigen showed no binding with secondary Abs. So finally we used competitive ELISA, it looks good though. Now it is the 2nd kit we are using, good product.from University of Texas at Austin,
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Great productVery easy to use, data are reliable and repeatable.from LSU,
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Great productGreat kit at an affordable cost. Easy to use. Have the option to choose Spike or ACE 2 on plate. Overnight incubation yields very high OD at 450nm, but the incubation time could be adjusted to 1-2hours at RT. It would be beneficial if they could provide the concentration of 1X ACE2 protein and also could have included a positive control (a known inhibitor) in the kit.from University of Mississippi,
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Great productInstructions are easy to follow. Works well with both kit-provided and our own RBD protein. Only suggestion is they should at least disclose the concentration of the query protein.from Scripps Research,
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Great productThe kit works as expected. Easy to use.from National Institutes of Health Clinical Center,
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Great productI ordered the kit when there was only one option, "Spike RBD on plate + ACE2 query (CoV-SACE2)". At that time, the storage condition was -20°C on arrival, keeping some at -20°C and some at 4°C. I mentioned this for we should take care of the storage of the components of the kit.from UMichigan,
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Great productWe obtained good results using this, so I'll be purchasing more. With that said, the instructions could have been written a bit clearer and the actual starting amount of ACE2 in vial F should be provided (phone operator said it was "unknown"). This makes assigning true values to the standard curve impossible. But as I said, the qualitative use for binding inhibition assays seems to work just fine, just can't be quantitative.from University of Nebraska Medical Center,
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Great productGreat kit. Easy to use. I love how the rows of wells can pop out of the plate so the unused ones can remain in the fridge.from Columbia University,
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Great productThe Spike-ACE2 binding assay is a sensitive and very suitable method for fast screening of potential inhibitors of the S-ACE2 complex formation. Signal intensity at 450 nm recorded for technical replicates results in very similar measurements and the signal-to-noise ratio is very high.from Pharma,
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