COVID-19 S-Protein (S1RBD) ELISA Kit

SARS-CoV-2 (COVID-19) S-Protein (S1RBD) ELISA Kit. Detects the S-Protein (S1RBD). Standard protein code: 230-30162.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

As low as $420.00
In stock
Only %1 left
Catalog #:
ELV-COVID19S1
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Product Description

Specifications

Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit, 10 Plate Kit
Gene Symbols S
Protein Name / Synonyms SARS-CoV-2 Spike Protein, S1 Subunit, S protein, spike protein
Quantitative/Semi-Quantitative Quantitative
Specificity This ELISA antibody pair detects COVID-19 S Protein (S1RBD).
Compatible Sample Types Cell Culture Supernatants, Serum
Solid Support 96-well Microplate
Method Of Detection Colorimetric
Design Principle Sandwich-based
Sensitivity 4.5 pg/ml
Detection Range 6.14 pg/ml - 1500 pg/ml
Estimated Lead Time 1-2 business days
Shipping Type Blue ice
Storage -20°C

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Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

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Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Great product
    Rating
    80%
    Kit functions as advertised. My one complaint is the cardboard holder that the reagents are shipped in. When I got my kit, all of the reagents had fallen out of their holders and were scattered throughout the box.

    Review by

    from University of California, San Diego (UCSD),

    on

  2. Great product
    Rating
    100%
    The kit arrived very quickly and was very easy to use following the manual. I used plasma samples from various groups of patients and the results came out very nicely!

    Review by

    from Boston Children's Hospital,

    on

  3. Great product
    Rating
    100%
    Got it very quickly after ordering and arrived in perfect condition. Would recommend!

    Review by

    from University of Arkansas,

    on

  4. Great product
    Rating
    100%
    After success with RayBiotech's N-Protein specific ELISA kit, the RBD specific kits proved to be just as easy to use. Human SARS-CoV-2 convalescent sera samples reacted well at several dilutions. These kits are also competitively priced compared to others. Standard curves are consistent and all reagents are clearly labeled and organized. Would use this kit again!

    Review by

    from Mayo Clinic,

    on

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