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    COVID-19 N-Protein ELISA Kit

    Rating:
    97 % of 100
    6  Reviews
    1 Citations

    RayBio® COVID-19 / SARS-CoV-2 Nucleocapsid Protein ELISA Kit. Detects the N-Protein (standard protein code: 230-01104).

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

    Documents
    As low as $420.00
    In stock
    Only %1 left
    Catalog #:
    ELV-COVID19N
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Gene Symbols N
    Protein Name / Synonyms Nucleoprotein, Nucleocapsid protein, Protein N
    Quantitative/Semi-Quantitative Quantitative
    Specificity This ELISA antibody pair detects virus COVID-19 N Protein
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum, Nasopharyngeal swab
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Sensitivity 0.07 ng/ml
    Detection Range 0.07 ng/ml - 50 ng/ml
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage -20°C

    Risk-Free Guarantee

    We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.

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    Amazon Gift Cards!

    $5 Amazon gift card in every kit box purchased.

    Product Features

    Additional Notes
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Citations

    Remsik J., Wilcox J., Babady NE., et al. Inflammatory Leptomeningeal Cytokines Mediate COVID-19 Neurologic Symptoms in Cancer Patients. Cancer Cell . 2021 Feb 8;39(2):276-283.e3. doi: 10.1016/j.ccell.2021.01.007. Epub 2021 Jan 16.
    Species:
    Human
    Sample Type:
    CSF (Inflammation in the brain during covid)
    View Publication

    Reviews

    Write a customer review
    1. Worked fine in detecting covid samples
      Rating
      100%
      Kit worked well with covid viral samples in supernatants down to nanogram quantities

      Review by

      on

    2. Great product
      Rating
      80%
      Kit works as intended. The cardboard reagent holder is not sturdy enough, so the reagents always fall out and get scattered throughout the box.

      Review by

      from University of California, San Diego (UCSD),

      on

    3. Great product
      Rating
      100%
      We have tested these SARS-CoV-2 N-specific protein ELISA kits to screen multiple COVID-19 convalescent donor plasma samples and found them as a reliable and sensitive assay to be used in both clinical and laboratory research settings.

      Review by

      from Mayo Clinic,

      on

    4. Great product
      Rating
      100%
      Very easy to use and accurate. Used with SARS-CoV-2 positive serum. Produces consistent controls and accurate replicates. Would definitely recommend!

      Review by

      from Mayo Clinic,

      on

    5. Great product
      Rating
      100%
      Human saliva samples, positive and negatives, accurate and robust.

      Review by

      from Oceanit Laboratories, Inc.,

      on

    6. Great product
      Rating
      100%
      Kit worked as described with several recombinant nucleocapsid proteins

      Review by

      from Aalto Scientific Ltd.,

      on

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