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    Bovine IGF-1 ELISA Kit

    Rating:
    95 % of 100
    4  Reviews

    RayBio® Bovine IGF-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

    Lead time: Typically ships within 1-2 business days. No Friday shipments.

    Documents
    As low as $480.00
    In stock
    Only %1 left
    Catalog #:
    ELB-IGF1
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human, Bovine (cow)
    Accession Number P07455
    Gene Id 281239
    Gene Symbols IGF1
    Protein Name / Synonyms Insulin-like growth factor I (IGF-I) (Somatomedin)
    Quantitative/Semi-Quantitative Quantitative
    Specificity This ELISA antibody pair detects a free form of Bovine IGF-1 and Human IGF-1 (not a complex such as binding with IGFBPs).
    Compatible Sample Types Cell Culture Supernatants, Plasma, Serum
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Sensitivity 1.23 ng/ml

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    Detection Range 1.23 ng/ml - 300 ng/ml
    Recommended Dilution (Serum/Plasma) 2 fold
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage ≤-20°C

    Risk-Free Guarantee

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    Product Features

    Additional Notes
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data

    Standard Curves

    Spiking & Recovery Results

    Linearity Results

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Other Materials Required
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Protocol Outline
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

    Storage/Stability

    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

    Reviews

    Write a customer review
    1. Easy and Simple
      Rating
      100%
      Great kit to use for bovine serum. Had simple and easy to read manual instructions that are online.

      Review by

      from Purina Animal Nutrition,

      on

    2. Research Technologist II
      Rating
      80%
      Bovine serum was used in this assay. It was an easy to ready and effective kit. Highly satisfied with the results.

      Review by

      from Purina Animal Nutrition,

      on

    3. Bovine IGF-1 ELISA Kit
      Rating
      100%
      Bovine IGF-1 ELISA Kit, this was an easy to use kit that was simple to follow. The online instructions were easy to follow and find on the website. We used it on Bovine samples. Can't wait to use the product again.

      Review by

      from Land O Lakes Purnia Animal Nutrition ,

      on

    4. Easy to Follow
      Rating
      100%
      The IGF ELISA was well constructed and had an easy to follow manual, making the process quick and simple.

      Review by

      from Purina Animal Nutrition,

      on

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