Bovine Cytokine Array Q30
Quantibody® Bovine Cytokine Antibody Array 30 Kit. A combination of 3 non-overlapping arrays to quantitatively measure 30 Bovine cytokines. Suitable for all liquid sample types.
Product Description
Specifications
Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
---|---|
Species | Bovine (cow) |
Quantitative/Semi-Quantitative | Quantitative |
Number of Targets Detected | 30 |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Solid Support | Glass Slide |
Method Of Detection | Fluorescence Laser Scanner |
Design Principle | Sandwich-based |
Estimated Lead Time | 1-2 business days |
Shipping Type | Blue ice |
Storage | -20°C |
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Scroll over each target protein for more information | ||||
---|---|---|---|---|
aFGF
(FGF-1)
|
Angiopoietin-1
|
bFGF
|
Decorin
|
|
GASP-1
(WFIKKNRP)
|
IFN alpha
|
IFN-gamma
|
IGF-1
|
|
IL-1 alpha
(IL-1 F1)
|
IL-13
|
IL-15
|
||
IL-17A
|
IL-2
|
IL-21
|
IL-36 Ra
(IL-1 F5)
|
|
IL-4
|
IP-10
(CXCL10)
|
MCP-1
(CCL2)
|
MIG
(CXCL9)
|
|
MIP-1 beta
(CCL4)
|
NCAM-1
(CD56)
|
RANTES
(CCL5)
|
TNF alpha
|
VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Bovine Cytokine Array Q30 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- 30 ml Centrifuge Tube
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.