The RayBiotech ATP Quantification Assay is a rapid, sensitive, and simple method that utilizes the ATP dependency of firefly luciferase to quantify ATP content in solution via the resulting luminosity.
The RayBio® ATP Quantification Assay is a rapid, sensitive, and simple method that utilizes the ATP dependency of firefly luciferase to quantify ATP content in solution via the resulting luminosity. This kit can be used to assess cell viability, cytotoxicity, and/or metabolic activity in a variety of media (cell culture, PBS, water, etc.) and can quantify as little as 0.064 picomoles in a 100 µL reaction.
Product Features
Rapid and Sensitive
Versatile Applications
High Sensitivity
Suitable for various cell types and environmental samples
Kit Components
Dilution Buffer
Assay Buffer
Lysis Buffer
ATP Standard
Luciferase
DTT
D-Luciferin
96-Well White-Walled Microplate
Plate Seal
Additional Materials Required
Sterile PBS
Vortex Mixer
Tabletop centrifuge
Pipettes and nuclease-free pipette tips (barrier tips recommended)
Serological pipette and nuclease-free glass tips
Nuclease-free 15 mL conical tubes and 1.5 mL microcentrifuge tubes
Plate reader capable of measuring luminosity
Assay Procedure Summary
Prepare all reagents, samples and standards as instructed.
Add standard or sample to each well of the microplate.
Add Master Mix to each well and mix.
Protect from light and allow to equilibrate to room temperature for 10 minutes.
Measure luminosity.
Storage/Stability
Store the kit at -70°C protected from light for up to 4 months. Kit must be used within expiry period to ensure optimal performance. Do not mix reagents from different kit lots.
