This antibody was produced from antiserum of rabbits immunized with a recombinant protein immunogen. The IgG fraction was purified by Protein A affinity chromatography.
Recommended Applications
ELISA (recommended work dilution = 1:5,000)
Reconstitution
Product is supplied as a powder obtained from lyophilization of purified antibody in glycine solution. Reconstitute the antibody with sterile water to a final concentration of 1 mg/ml.
N7-methylguanosine (m7G) is the most ubiquitous mRNA cap modification and is also present in internal mRNA, microRNA (miRNA), tRNA and rRNA. m7G is positively charged and produced by the methyltransferase-like 1 (METTL1) and WD repeat domain 4 (WDR4) complex. Emerging evidence suggests that the METTL1/WDR4 complex promoted or inhibited the processes of brain development and diseases, as well as many kinds of tumor, including head and neck, lung, liver, colon, bladder cancer, and teratoma. Additionally, m7G is also crucial for tumor chemoresistance, vasculogenesis and prognosis. Therefore, m7G modification can be used as biomarkers or potential intervention targets, providing new possibilities for early diagnosis and treatment of tumors.
Storage/Stability
Store at -20°C. If intended for use within a month, may keep at 4°C. Minimize freeze-thaw cycles.
Expiration
12 months from the date of shipment when stored properly.
