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This kit is a sandwich-based bridging ELISA for the quantitative measurement of Adalimumab in serum, plasma, and cell culture supernatants.
Product Description
Specifications
Size
1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Protein Name / Synonyms
Adalimumab
Quantitative/Semi-Quantitative
Quantitative
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum
Solid Support
96-well Microplate
Method Of Detection
Colorimetric
Design Principle
Sandwich-based
Research Area
Antibody Drug Development, Drug Antibody (DA) Detection
Sensitivity
0.012 ng/ml
Detection Range
0.037 ng/ml - 9 ng/ml
Recommended Dilution (Serum/Plasma)
8 fold
Estimated Lead Time
1-2 business days
Shipping Type
Blue ice
Storage
≤-20°C
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Standard Curve
Application Notes
Kit Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Stop Solution
Assay Diluent
Other Materials Required
Microplate reader capable of measuring absorbance at 450nm
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 ml and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Log-log graph paper or computer and software for ELISA data analysis
Horizontal orbital microplate shaker
Tubes to prepare standard and sample dilutions
Protocol Outline
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well. Incubate 2 hours at room temperature.
Add 100 μl prepared biotinylated antibody to each well. Incubate 1 hour at room temperature.
Add 100 μl prepared Streptavidin solution. Incubate 45 minutes at room temperature.
Add 100 μl TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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