Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Rat
Accession Number	Q63264
Gene Symbols	IL1B
Protein Name / Synonyms	Interleukin-1 beta (IL-1 beta)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: rat CINC-2, CINC-3, CNTF, Fractalkine, IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-10, GM-CSF, IFN-gamma, Leptin, Lix, MCP-1, MIP-3 alpha, beta- NGF, TIMP-1, TNF-alpha.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	80 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	80 pg/ml - 50000 pg/ml
Recommended Dilution (Serum/Plasma)	2 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELR-IL1b.pdf)

Datasheet (ELR-IL1b-CL.pdf)

SDS (ELR-IL1b.pdf)

SDS (ELR-IL1b-CL.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Allabassi M. The Possible Protective Effects of Zileuton against Pulmonary Fibrosis Induced by Amiodarone in Male Rats. Journal of Natural Sciences Research. Vol.5, No.2, 2015

Species:

Rat

Sample Type:

Tissue Lysate (Lung tissue treated with Zileuton)

View Publication

Abdelkader NF., Safar MM., Salem H. Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson's Disease: Modulation of Mitochondrial Perturbations. Mol Neurobiol. 2014 Dec 15. [Epub ahead of print]. DOI 10.1007/s12035-014-9043-8

Species:

Rat

Sample Type:

Tissue Lysate (Striatal brain tissue samples)

View Publication

Hedya SA, Safar MM, Bahgat AK. Cilostazol Mediated Nurr1 and Autophagy Enhancement: Neuroprotective Activity in Rat Rotenone PD Model. Mol Neurobiol. 2018 Sep;55(9):7579-7587. doi: 10.1007/s12035-018-0923-1. Epub 2018 Feb 10. PMID: 29429051.

Species:

Rat

Sample Type:

Tissue Lysate (Striatal Tissue)

View Publication

Chen H., Min XH., Wang QY., et al. Pre-activation of mesenchymal stem cells with TNF-?, IL-1? and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury. Scientific Reports 5, Article number: 8718 (2015). doi:10.1038/srep08718

Species:

Rat

Sample Type:

Serum

View Publication

Chen H., Min XH., Wang QY., et al. Pre-activation of mesenchymal stem cells with TNF-?, IL-1? and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury. Scientific Reports 5, Article number: 8718 (2015). doi:10.1038/srep08718

Species:

Rat

Sample Type:

Tissue Lysate (Intestinal Tissue lysates)

View Publication

JÃ¤rvelÃ¤, Juha T., et al. "Temporal profiles of age-dependent changes in cytokine mRNA expression and glial cell activation after status epilepticus in postnatal rat hippocampus." Journal of neuroinflammation 8.1 (2011): 29.

Species:

Rat

Sample Type:

Tissue Lysate (hipocampus)

View Publication

Meireles M., Marques C., Norberto S., et al. The impact of chronic blackberry intake on the neuroinflammatory status of rats fed a standard or high-fat diet. J Nutr Biochem. 2015 Nov;26(11):1166-73. doi: 10.1016/j.jnutbio.2015.05.008.

Species:

Rat

Sample Type:

Tissue Lysate (Cortex brain tissue lysate)

View Publication

Li Y, Li S, Li D. Breviscapine Alleviates Cognitive Impairments Induced by Transient Cerebral Ischemia/Reperfusion through Its Anti-Inflammatory and Anti-Oxidant Properties in a Rat Model. ACS Chem Neurosci. 2020 Dec 16;11(24):4489-4498. doi: 10.1021/acschemneuro.0c00697. Epub 2020 Dec 3. PMID: 33270442.

Species:

Rat

Sample Type:

Serum (MCAO rat model)

View Publication

Arya, Aditya, et al. "Cerium oxide nanoparticles protect rodent lungs from hypobaric hypoxia-induced oxidative stress and inflammation." International journal of nanomedicine 8 (2013): 4507.

Species:

Rat

Sample Type:

Plasma

View Publication

Xie, Y., Feng, S.-L., Mai, C.-T., Zheng, Y.-F., Wang, H., Liu, Z.-Q., Liu, L. (2021). Suppression of up-regulated LXR? by silybin ameliorates experimental rheumatoid arthritis and abnormal lipid metabolism. Phytomedicine, 80, 153339. doi:10.1016/j.phymed.2020.153340

Species:

Rat

Sample Type:

Serum

View Publication

View All Publications For This Product

Reviews

Write a customer review

it worked for Rat plasma samples

Rating
60%

Easy to use and detect the IL-6 from Rat plasma samples

Review by Eunhee C,

from University of Texas at San Antonio,

on May 26, 2023
Great product

Rating
100%

We preformed this experiment on brain cell lysates and plasma extracted from rat

Review by Vicki S,

from UNMC,

on Feb 28, 2023
Great product

Rating
100%

I used rat serum for the analysis. The assay procedure was simple and reagents quantities were perfect. The assay was easy to use and sensitive, I got the reliable results compare to the from the previous experiments.

Review by Mani,

from Iowa state University,

on Mar 13, 2022
Great product

Rating
100%

I used rat plasma (heparin anti coagulated) and kidney lysate. After determining an appropriate dilution, using two lanes of the plate, went on to determine the concentrations present in our sample. The assay was easy to use and sensitive, corroborating results from other experiments.

Review by Nancy,

from University of Wisconsin,

on Feb 25, 2021
Great product

Rating
100%

Excellent results. Easy to use. Reproducible.

Review by Kay Beharry,

from SUNY Downstate Medical Center,

on Jul 30, 2019
Great product

Rating
100%

The Rat IL-1B ELISA kit from RayBiotech was easy to use and worked very well. The manufacturer protocol was simple and very user friendly. I recommend using this kit for detecting IL-1b in tissue lysates.

Review by Asheebo Rojas,

from Emory University,

on Apr 7, 2019
Great product

Rating
80%

Worked well for our pancreas homogenates

Review by Anonymous,

on Feb 12, 2019

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