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    Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit

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    2 Citations

    RayBio® Human/Mouse/Rat Phospho-Erk1 (T202/Y204) + Phospho-Erk2 (T185/Y187) ELISA Kit. This assay semi-quantitatively measures phosphorylated Erk1 (Thr202/Tyr204) + in lysate samples.

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    Catalog #:
    PEL-Erk-T202
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    Product Description

    Specifications

    Size 1 Plate Kit, 2 Plate Kit, 5 Plate Kit
    Species Human, Mouse, Rat
    Accession Number P27361|P28482
    Gene Id 5595 and 5594
    Gene Symbols MAPK1|MAPK3|ERK1|PRKM3|ERK2|PRKM1|PRKM2|AND
    Protein Name / Synonyms Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (Microtubule-associated protein 2 kinase) (p44-ERK1) Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2)
    Quantitative/Semi-Quantitative Semi-Quantitative
    Specificity The antibody pair provided in this kit recognizes human, mouse and rat Phospho-Erk1 (pThr202/pTyr204) + Erk2 (pTyr185/187).
    Compatible Sample Types Tissue Lysates, Cell Lysates
    Solid Support 96-well Microplate
    Method Of Detection Colorimetric
    Design Principle Sandwich-based
    Research Area Post-Translational Modifications, Phosphorylation, MAPK Signaling
    Estimated Lead Time 1-2 business days
    Shipping Type Blue ice
    Storage -20°C

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    Product Features

    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material

    Application Notes

    Kit Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Other Materials Required
    • Distilled or deionized water
    • 100 ml and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 µl to 1 ml volumes
    • Adjustable 1-25 ml pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Protocol Outline
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 µl of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 µl of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Storage/Stability

    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

    Citations

    Stephens, Alexandre S., et al. "Myocyte enhancer factor 2c, an osteoblast transcription factor identified by dimethyl sulfoxide (DMSO)-enhanced mineralization." Journal of Biological Chemistry 286.34 (2011): 30071-30086.
    Species:
    Human
    Sample Type:
    Conditioned Media
    View Publication
    Sustarsic E., Junnila R., Kopchick J. Human metastatic melanoma cell lines express high levels of growth. Biochemical and Biophysical Research Communications 441 (2013) 144-150hormone receptor and respond to GH treatment
    Species:
    Human
    Sample Type:
    Cell Lysate (Human melanoma cell line, CBEL kit)
    View Publication

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