Mouse IGF-1 ELISA Kit

Name: Mouse IGF-1 ELISA Kit
Brand: ELM-IGF1
SKU: ELM-IGF1
Price: 336 USD
Availability: InStock
Rating: 93 (3 reviews)

Rating:

93 % of 100

3 Reviews

5 Citations

RayBio® Mouse IGF-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

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As low as $336.00 Regular Price $420.00

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Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Mouse
Accession Number	P05017
Gene Id	16000
Gene Symbols	IGF1\|IGF-1
Protein Name / Synonyms	Insulin-like growth factor I (IGF-I) (Somatomedin)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP- 5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, PSelectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Sensitivity	4 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	4 pg/ml - 2000 pg/ml
Recommended Dilution (Serum/Plasma)	100 - 5,000 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Target Background

Insulin-like-growth factor is a hormone structurally similar to insulin. It is central to the somatotropic axis acting downstream of growth hormone (GH) to promote tissue growth and development. IGF1 is synthesized in the liver, where its secretion is regulated by the pituitary secretion of GH. The actions of IGF1 are mediated through the IGF1R receptor to activate downstream signaling cascades such as PI3-kinase and mitogen-activated protein kinase pathways. Defects in the encoding gene can cause insulin-like growth factor deficiency - characterized by growth retardation, sensorineural deafness and intellectual disability.

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELM-IGF1.pdf)

SDS (ELM-IGF1.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Tebebi P., Burks S., Kim S., et al. Cyclooxygenase-2 or Tumor Necrosis Factor-a Inhibitors Attenuate the Mechanotransductive Effects of Pulsed Focused Ultrasound to Suppress Mesenchymal Stromal Cell Homing to Healthy and Dystrophic Muscle. Stem Cells 2015 Apr;33(4):1173-86. doi: 10.1002/stem.1927

Species:

Mouse

Sample Type:

Tissue Lysate (Hamstring tissue lysate)

View Publication

Hamdi, Hadhami, et al. "Efficacy of epicardially delivered adipose stroma cell sheets in dilated cardiomyopathy." Cardiovascular research 99.4 (2013): 640-647.

Species:

Mouse

Sample Type:

Tissue Lysate (dilated cardiomyopathy model)

View Publication

Juffer P., Jaspers RT., Lips P., et al. Expression of muscle anabolic and metabolic factors in mechanically loaded MLO-Y4 osteocytes. Am J Physiol Endocrinol Metab. 2012 Feb 15;302(4):E389-95. doi: 10.1152/ajpendo.00320.2011.

Species:

Mouse

Sample Type:

Conditioned Media (MLO-Y4 osteocytes cells)

View Publication

Huey K., Smith S., Sulaeman A., Breen E. Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice. J Appl Physiol (1985). 2016 Jan 15;120(2):188-95. doi: 10.1152/japplphysiol.00638.2015.

Species:

Mouse

Sample Type:

Tissue Lysate (Skeletal muscle lysate from plantaris muscle)

View Publication

Provenzano G., et al. GH dysfunction in Engrailed-2 knockout mice, a model for autism spectrum disorders. Frontiers in Pediatrics. published: 01 September 2014doi: 10.3389/fped.2014.00092

Species:

Mouse

Sample Type:

Serum

View Publication

Reviews

Write a customer review

Great product

Rating
80%

I used this kit to detect serum IGF1 level in irradiated and non-irradiated control mice.

Review by Kamendra Kumar,

from Georgetown University,

on Jan 8, 2023
Great product

Rating
100%

The kit worked well with the serum samples from mice of C57BL/6J background

Review by Artem Astafev,

from Cleveland State University,

on Feb 13, 2020
Great product

Rating
100%

Works with mouse serum, both adult and pup, and worked with our milk samples. Great ELISA!

Review by Zachary Carlson,

from University of Michigan,

on Feb 24, 2019

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Mouse IGF-1 ELISA Kit