RayBio® Human MCP-1 (CCL2) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Human |
| Accession Number | P13500 |
| Gene Id | 6347 |
| Gene Symbols | CCL2|SCYA2|MCP1 |
| Protein Name / Synonyms | C-C motif chemokine 2 (HC11) (Monocyte chemoattractant protein 1) (Monocyte chemotactic and activating factor) (MCAF) (Monocyte chemotactic protein 1) (MCP-1) (Monocyte secretory protein JE) (Small-inducible cytokine A2) |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Design Principle | Sandwich-based |
| Sensitivity | 2 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 2 pg/ml - 500 pg/ml |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Risk-Free Guarantee
We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.
Learn More
Amazon Gift Cards!
$5 Amazon gift card in every kit box purchased.
Related Products
Mause S., Ritzel E., Liehn E., et al. Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury. Circulation . 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473.
Caspar-Bauguil S., Kolditz C., Lefort C., et al. Fatty acids from fat cell lipolysis do not activate an inflammatory response but are stored as triacylglycerols in adipose tissue macrophages. Diabetologia. 2015 Nov;58(11):2627-36. doi: 10.1007/s00125-015-3719-0.
Lv M., et al. miR141-CXCL1-CXCR2 Signaling-Induced Treg Recruitment Regulates Metastases and Survival of Non-Small Cell Lung Cancer. Molecular Cancer Therapeutics. OnlineFirst October 27, 2014; doi: 10.1158/1535-7163.MCT-14-0448
Perisdsky Y., Hill J., Zhang M., et al. Dysfunction of brain pericytes in chronic neuroinflammation. J Cereb Blood Flow Metab. 2016 Apr;36(4):794-807. doi: 10.1177/0271678X15606149.
Wang J., Su X., Yang L., et al. The influence of myeloid-derived suppressor cells on angiogenesis and tumor growth after cancer surgery. Int J Cancer. 2016 Jun 1;138(11):2688-99. doi: 10.1002/ijc.29998.
Sambataro M, Sambado L., Trevisiol E., et al. Proinsulin-expressing dendritic cells in type 2 neuropathic diabetic patients with and without foot lesions. FASEB J. 2018 Feb 12:fj201701279RR. doi: 10.1096/fj.201701279RR
Chen YL., Huang KF., Kuo WC., et al. Inhibition of glutaminyl cyclase attenuates cell migration modulated by monocyte chemoattractant proteins. Biochem. J. (2012) 442, 403-412. doi:10.1042/BJ20110535
Filková M, Hulejová H, Kuncová K, et al. Resistin in idiopathic inflammatory myopathies. Arthritis Research & Therapy. 2012;14(3):R111. doi:10.1186/ar3836.
Leow K-Y, Goh WWB, Tan S-Z, et al. Variant screening of the serum amyloid A1 gene and functional study of the p.Gly90Asp variant for its role in atherosclerosis. Atherosclerosis. 2013 Mar;227(1):112-117.
Huggins C., Pearce S., Peri F., et al. A novel small molecule TLR4 antagonist (IAXO-102) negatively regulates non-hematopoietic toll like receptor 4 signalling and inhibits aortic aneurysms development. Atherosclerosis. 2015 Oct;242(2):563-70. doi: 10.1016/j.atherosclerosis.2015.08.010
-
Great productI used this kit on human patient ascites samples. The results were consistent with previous analysis on a cytokine array and I was pleased with the results. My only complaint is the protocol is not written very clearly. Specifically, the assay diluent (item E2) needs to be diluted 5-fold prior to use. I have used several RaybioTech ELISAs before and this was the first one that required the buffer to be diluted first. That in and of itself wouldn't be an issue, however, the item names are "Item E" for the 1x buffer and "Item E2" for the 5x buffer. Since I was used to using the kits that have 1x buffer, I missed the dilution step and ruined the entire assay. The customer service representative was a life saver, she responded extremely quickly and had a free replacement kit in the mail the next day. Overall, I am pleased with the product but do wish the protocols were clearer and the item names were more specific to avoid confusion.
from University of New Mexico,
on
Great productThe Human MCP-1 ELISA was used to test the levels of secreted Monocyte Chemoattractant Protein-1 upon treatment of cancer cells with chemotherapeutics. Results were consistent.from AUSTRALIS PHARMACEUTICALS,
on
Great productGreat kit! Protocol is fairly standard with all Raybiotech kits which makes it simple to work with.from University of Houston,
on
Great productUsed for analysis of human urine samples, worked perfectly.from NephroNet,
on
Great productI am extremely happy with the results obtained using this kit. I used it before in many studies to measure the cytokine in the supernatant. You might need to try different concentrations to get the best results.from Florida A&M University,
on
Great productEasy and precisefrom University of Houston,
on
Great productEasy to follow!from Oregon Health & Science University,
on
Great productVery easy instructions to follow and the kit worked very well.from University of Houston,
on
Great productEasy to follow and works exactly as expected.from Biocogent, LLC,
on
Great productUsed for analysis of human serum samples, easy to use, clean definitive results for varied human samples.from Washington University,
on
Write Your Own ReviewAsk a Question