Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit, 10 Plate Kit
Species	Human
Accession Number	P10145
Gene Id	3576
Gene Symbols	CXCL8\|IL8
Protein Name / Synonyms	Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I) Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b) IL-8(5-77) IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF) IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV) IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V) IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)]
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Research Area	Antibody Drug Development, Protein Therapeutics, Biomarker Detection
Sensitivity	1 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	1 pg/ml - 600 pg/ml
Recommended Dilution (Serum/Plasma)	2 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

Risk-Free Guarantee

We offer a 100% guarantee on all ELISA kits and membrane cytokine arrays.

Learn More

Amazon Gift Cards!

$5 Amazon gift card in every kit box purchased.

Target Background

Interleukin 8 (IL-8) is a chemotactic factor that attracts neutrophils, basophils and T-cells during an inflammatory process. It is released from a variety cell types such as monocytes, macrophages, neutrophils and intestine, kidney, placenta and bone marrow cells in response to inflammation. It is involved many cellular processes including cell proliferation, tissue remodeling and angiogenesis. IL-8 mediates its effects by binding to two heterotrimeric G protein-coupled receptors, CXCR1 and CXCR2. Given that it is a major mediator of inflammation, IL-8 was markedly increased in infants diagnosed with perinatal infection.

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELH-IL8.pdf)

Datasheet (ELH-IL8-CL.pdf)

SDS (ELH-IL8.pdf)

SDS (ELH-IL8-CL.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Mause S., Ritzel E., Liehn E., et al. Platelet Microparticles Enhance the Vasoregenerative Potential of Angiogenic Early Outgrowth Cells After Vascular Injury. Circulation . 2010 Aug 3;122(5):495-506. doi: 10.1161/CIRCULATIONAHA.109.909473.

Species:

Human

Sample Type:

Conditioned Media (HUVEC cells in vascular injury model)

View Publication

Dominguez C., McCampbell K., David J., Palena C. Neutralization of IL-8 decreases tumor PMN-MDSCs and reduces mesenchymalization of claudin-low triple-negative breast cancer. JCI Insight. 2017 Nov 2;2(21). pii: 94296. doi: 10.1172/jci.insight.94296.

Species:

Human

Sample Type:

Conditioned Media (Breast cancer cell lines including MDB-MB-231 and MCF7 among others)

View Publication

Zhao D., Najbauer J., Annala AJ., et al. Human neural stem cell tropism to metastatic breast cancer. Stem Cells. 2012 Feb;30(2):314-25. doi: 10.1002/stem.784.

Species:

Human

Sample Type:

Conditioned Media (MDA-MB-231 breast cancer cells)

View Publication

Wang XS, Williams LA, Krishnan S, et al. Serum sTNFR1, IL6, and the Development of Fatigue in Patients with Gastrointestinal Cancer Undergoing Chemoradiation Therapy. Brain, Behavior, and Immunity. 2012;26(5):699-705. doi:10.1016/j.bbi.2011.12.007.

Species:

Human

Sample Type:

Serum (Colorectal or esphageal cancer patients treated with concurrent chemoradiation therapy)

View Publication

LaValle CR, Zhang L, Xu S, Eiseman JL, Wang QJ. Inducible silencing of protein kinase D3 inhibits secretion of tumor-promoting factors in prostate cancer. Molecular Cancer Therapeutics 2012;11(7):1389-1399. doi:10.1158/1535-7163.MCT-11-0887.

Species:

Human

Sample Type:

Conditioned Media (PC3, prostate cancer cell lines)

View Publication

Lv M., et al. miR141-CXCL1-CXCR2 Signaling-Induced Treg Recruitment Regulates Metastases and Survival of Non-Small Cell Lung Cancer. Molecular Cancer Therapeutics. OnlineFirst October 27, 2014; doi: 10.1158/1535-7163.MCT-14-0448

Species:

Human

Sample Type:

Conditioned Media (Non-small cell lung cancer)

View Publication

Lu H., et al. A breast cancer stem cell niche supported by juxtacrine signalling from monocytes and macrophages. Nature Cell Biology (2014) doi:10.1038/ncb3041

Species:

Human

Sample Type:

Conditioned Media (Mammary Epithelial Cell lines)

View Publication

Zhou X., Gu J., Gu Y., et al. Human Umbilical Cord-Derived Mesenchymal Stem Cells Improve Learning and Memory Function in Hypoxic-Ischemic Brain-Damaged Rats via an IL-8-Mediated Secretion Mechanism Rather than Differentiation Pattern Induction. Cell Physiol Biochem. 2015;35(6):2383-401. doi: 10.1159/000374040

Species:

Human

Sample Type:

Conditioned Media (Human MSC cells)

View Publication

Xia, Yiru, et al. "mTOR inhibition rejuvenates the aging gingival fibroblasts through alleviating oxidative stress." Oxidative medicine and cellular longevity 2017 (2017).

Species:

Human

Sample Type:

Conditioned Media (gingival fibroblasts)

View Publication

Grbic DM, Degagne E, Langlois C, Dupuis A, Gendron F. Intestinal Inflammation Increases the Expression of the P2Y6 Receptor on Epithelial Cells and the Release of CXC Chemokine Ligand 8 by UDP. J Immunol. 2008;180:2659-2668.

Species:

Human

View Publication

View All Publications For This Product

Reviews

Write a customer review

Great product

Rating
100%

It was a convenient assay to follow, and showed accuracy in the triplicates.

Review by Ozcan,

from University of Connecticut,

on Jun 3, 2022
Great product

Rating
100%

Human Cell Culture SupernatantsCell type was used.

Review by Dan,

from NanoBST,

on Feb 27, 2022
Great product

Rating
100%

Great kit, one of the standards in our lab. Easy to use, and customer service is great.

Review by Laura,

from UTSW,

on Feb 17, 2022
Great product

Rating
100%

I ordered this kit for many studies and it works very good for me. I tried different dilution factors.

Review by Samia,

from Florida A&M University,

on Dec 17, 2020
Great product

Rating
100%

Used IL8 for Elisa to identify Neutrophils. Easy to follow instructions.

Review by Vlad Strumkovsky,

from Husson University,

on Sep 2, 2020
Great product

Rating
80%

I have used this kit with tissue as well as supernatant and it's really easy

Review by Aida,

from CPMC,

on Jul 8, 2020
Great product

Rating
80%

I used this kit a couple of times in cell lysate and it works really good

Review by Aida,

from CPMC,

on Jun 1, 2020
Great product

Rating
100%

I used this kit to measure the IL-8 secretion in culture supernatant. The kit has easy to follow instructions. It worked really great.

Review by Richa,

from Icahn School of Medicine at Mount Sinai,

on Jan 12, 2020
Great product

Rating
100%

I bought this IL8 ELISA 3 times from RayBiotech, each time the very exact results for same samples with a range of 1-600 pg/ml. I am impressed.

Review by Madhavi Pali,

from University of Texas Dallas,

on Sep 23, 2019
Great product

Rating
80%

0

Review by Luvina Rice,

from University of Texas Dallas,

on Aug 26, 2018
Great product

Rating
100%

Easy to follow and works exactly as expected.

Review by Brianna,

from Biocogent, LLC,

on Jul 24, 2018
Great product

Rating
100%

Best Elisa. Easy to use instructions very helpful for new users.

Review by Debbie Piktel,

from Wvu,

on Jun 30, 2018
Great product

Rating
100%

It is very user-friendly well written protocol. Easy to use and get results in one day.

Review by Dhara Shah,

from University of Illinois at Chicago,

on Jun 20, 2018
Great product

Rating
80%

I used this kit to measure IL-8 in culture supernatants after HIV treatment. The results look good and there was no high background in my negative controls.

Review by Nancy,

on Apr 16, 2018

Write Your Own Review

Your Rating

1 star 2 stars 3 stars 4 stars 5 stars

Display Name

Your email address is required to receive a $10 Amazon Gift Card. It will not be posted with the review. Gift cards are emailed out every Friday.

Review Title / Summary

Institution

Review (include details such as sample type used, experimental conditions, etc.)

Submit a review for this product on Biocompare.com to earn an entry into a monthly raffle for one of five $100 Amazon gift cards!

Ask a Question

Display name:

Your e-mail:*

Your message (include experimental conditions or sample type used):*

Your location:*

U.S.

Non-U.S.

Name

This field is for validation purposes and should be left unchanged.

Featured products

Human IL-8 ELISA Kit