Human IL-18 ELISA Kit
RayBio® Human IL-18 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Human |
| Accession Number | Q14116 |
| Gene Id | 3606 |
| Gene Symbols | IL18|IGIF|IL1F4 |
| Protein Name / Synonyms | Interleukin-18 (IL-18) (Iboctadekin) (Interferon gamma-inducing factor) (IFN-gamma-inducing factor) (Interleukin-1 gamma) (IL-1 gamma) |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Design Principle | Sandwich-based |
| Sensitivity | 0.5 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 0.5 pg/ml - 75 pg/ml |
| Recommended Dilution (Serum/Plasma) | 5 - 50 fold |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Target Background
Interleukin-18 (IL-18) is a member of the IL-1 family of cytokines. Similar to IL-1b, it is synthesized as an inactive precursor which requires processing by caspase-1. However, unlike IL-1b, IL-18 is constitutively active in nearly all healthy cells. IL-18 binding protein (IL-18BP) has high affinity for IL-18, thus mediating the activity of IL-18. IL-18 forms a signaling complex with the IL-18Rα receptor and IL-18Rβ co-receptor to mediate NF?B pro-inflammatory signaling. IL-18BP, present in the extracellular compartment, binds mature IL-18 preventing binding to the IL-18 receptor. IL-18 has been implicated in conditions such as autoimmune disease, myocardial infarction, metabolic syndromes, inflammatory bowel disease and psoriasis.
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Unamuno X, Gómez-Ambrosi J, RamÃrez B, RodrÃguez A, Becerril S, Valentà V, Moncada R, Silva C, Salvador J, Frühbeck G, Catalán V. NLRP3 inflammasome blockade reduces adipose tissue inflammation and extracellular matrix remodeling. Cell Mol Immunol. 2019 Sep 24. doi: 10.1038/s41423-019-0296-z. Epub ahead of print. PMID: 31551515.
Species:
Human
Sample Type:
Conditioned Media
Shiori Yoshikawa, Sachiyo Yoshio, Yuichi Yoshida, Yuriko Tsutsui, Hironari Kawai, Taiji Yamazoe, Taizo Mori, Yosuke Osawa, Masaya Sugiyama, Masashi Iwamoto, Koichi Watashi, Takumi Kawaguchi, Tomoyuki Akita, Junko Tanaka, Yoshimi Kikuchi, Masashi Mizokami, Shinichi Oka, Tatsuya Kanto, Hiroyuki Gatanaga, Impact of Immune Reconstitution-Induced Hepatic Flare on Hepatitis B Surface Antigen Loss in Hepatitis B Virus/Human Immunodeficiency Virus-1 Coinfected Patients, The Journal of Infectious Diseases, , jiaa662, https://doi.org/10.1093/infdis/jiaa662
Species:
Human
Sample Type:
Serum
Al-Hakeim H., Al-Rammahi D., Al-Dujaili A. IL-6, IL-18, sIL-2R, and TNF? proinflammatory markers in depression and schizophrenia patients who are free of overt inflammation. J Affect Disord. 2015 Aug 15;182:106-14. doi: 10.1016/j.jad.2015.04.044
Species:
Human
Sample Type:
Serum (Depressed Patients)
Li X., Yan X., Wang Y., et al. NLRP3 inflammasome inhibition attenuates silica-induced epithelial to mesenchymal transition (EMT) in human bronchial epithelial cells. Exp Cell Res. 2018 Jan 15;362(2):489-497. doi: 10.1016/j.yexcr.2017.12.013.
Species:
Human
Sample Type:
Conditioned Media (Human Bronchial epithelial cells in silica model)
Guo W., Liu W., Chen Z., et al. Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis. Nat Commun. 2017 Dec 18;8(1):2168. doi: 10.1038/s41467-017-02351-0.
Species:
Mouse
Sample Type:
Conditioned Media (Macrophages that don't express or have SHP2 blocked)
Gileles-Hillel A., Alonso-Alvares M., Kheirandish-Gozal L., Peris E., et al. Inflammatory markers and obstructive sleep apnea in obese children: The NANOS Study. Mediators of Inflammation. Accepted May 2014 http://www.hindawi.com/journals/mi/aip/605280/ [Epub ahead of print]
Species:
Human
Sample Type:
Plasma (Plasma)
Szpakowski P., et al. Dendritic cell activity driven by recombinant Mycobacterium bovis BCG producing human IL-18, in healthy BCG vaccinated adults. Journal of Immunology Research Sept 2014, epub ahead of print.
Species:
Human
Sample Type:
Conditioned Media (DC supernate)
Jaiswal, Praveen Kumar, et al. "Association of IL-12, IL-18 variants and serum IL-18 with bladder cancer susceptibility in North Indian population." Gene 519.1 (2013): 128-134.
Species:
Human
Sample Type:
Serum (bladder cancer patients)
Pecorelli A, Cordone V, Messano N, Zhang C, Falone S, Amicarelli F, Hayek J, Valacchi G. Altered inflammasome machinery as a key player in the perpetuation of Rett syndrome oxinflammation. Redox Biol. 2020 Jan;28:101334. doi: 10.1016/j.redox.2019.101334. Epub 2019 Oct 6. PMID: 31606551; PMCID: PMC6812177.
Species:
Human
Sample Type:
Serum (female patients with clinical diagnosis of typical RTT and MECP2 mutation and healthy controls)
Han Y, Xu X, Tang C, Gao P, Chen X, Xiong X, Yang M, Yang S, Zhu X, Yuan S, Liu F, Xiao L, Kanwar YS, Sun L. Reactive oxygen species promote tubular injury in diabetic nephropathy: The role of the mitochondrial ros-txnip-nlrp3 biological axis. Redox Biol. 2018 Jun;16:32-46. doi: 10.1016/j.redox.2018.02.013. Epub 2018 Feb 15. Erratum in: Redox Biol. 2019 Jun;24:101216. PMID: 29475133; PMCID: PMC5842313.
Species:
Human/mouse
Sample Type:
(HK-2 Cells)
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Great productEasy to use. Reliable. Not a fan of the 8 well strips.
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