Human G-CSF ELISA Kit
RayBio® Human GCSF ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
|---|---|
| Species | Human |
| Accession Number | P09919 |
| Gene Id | 1440 |
| Gene Symbols | CSF3|C17ORF33|GCSF |
| Protein Name / Synonyms | Granulocyte colony-stimulating factor (G-CSF) (Pluripoietin) (Filgrastim) (Lenograstim) |
| Quantitative/Semi-Quantitative | Quantitative |
| Specificity | This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Solid Support | 96-well Microplate |
| Method Of Detection | Colorimetric |
| Design Principle | Sandwich-based |
| Sensitivity | 1 pg/ml Need more sensitivity? Check out the new BIQ-ELISAâ„¢ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 1 pg/ml - 500 pg/ml |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Estimated Lead Time | 1-2 business days |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Easy-to-Use, but not specific enoughI used the RayBio Human G-CSF ELISA Kit recently to measure the amount of G-CSF in mouse serum samples. I chose this kit mainly because the standard curve was within the range of the protein levels typically seen in our samples. The kit was easy to use - everything was labeled clearly and the manual was very easy to follow and understand. The only downside of the procedure was that it was very long (~5 hours long). The only issue is that I do not think the kit was specific enough for human G-CSF. When I read the plate with our samples, there was some detection of G-CSF from the negative control that we ran, which is not supposed to happen. We think that that may be because it is detecting some mouse G-CSF within the sample. That being said, I will still definitely recommend this kit to those who are looking for human G-CSF detection in their samples.
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