Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Species	Human
Accession Number	P09038
Gene Id	2247
Gene Symbols	FGF2\|FGFB
Protein Name / Synonyms	Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Quantitative/Semi-Quantitative	Quantitative
Specificity	This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Solid Support	96-well Microplate
Method Of Detection	Colorimetric
Design Principle	Sandwich-based
Research Area	Angiogenesis
Sensitivity	50 pg/ml Need more sensitivity? Check out the new BIQ-ELISA™ kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	50 pg/ml - 10000 pg/ml
Recommended Dilution (Serum/Plasma)	2 fold
Estimated Lead Time	1-2 business days
Shipping Type	Blue ice
Storage	≤-20°C

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Target Background

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, is a member of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (HST/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Need your results faster? Try RayBiotech's SpeedELISA platform for quantitative detection in just three hours.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Documents

Datasheet (ELH-bFGF.pdf)

Datasheet (ELH-bFGF-CL.pdf)

SDS (ELH-bFGF.pdf)

SDS (ELH-bFGF-CL.pdf)

Excel (sandwich-elisa.xlsm)

Citations

Fedak PW, Bai L., Turnbull J., et al. Cell therapy limits myofibroblast differentiation and structural cardiac remodeling: basic fibroblast growth factor-mediated paracrine mechanism. Circ Heart Fail. 2012 May 1;5(3):349-56. doi: 10.1161/CIRCHEARTFAILURE.111.965889

Species:

Human

Sample Type:

Tissue Lysate (Vascular Smooth Muscle Cell Lysates)

View Publication

Lai H., Kuan C., Wu H., Tsai J., Chen T., Hsieh D., Wang T. Tailor Design Electrospun Composite Nanofibers with Staged Release of Multiple Angiogenic Growth Factors for Chronic Wound Healing. Acta Biomaterialia Available online May 9, 2014. http://dx.doi.org.proxy.library.emory.edu/10.1016/j.actbio.2014.05.001 [Epub ahead of print]

Species:

Human

Sample Type:

Nanomaterials (Nanomaterials incorporation of bFGF)

View Publication

Go, Dewi P., et al. "Multilayered microspheres for the controlled release of growth factors in tissue engineering." Biomacromolecules 12.5 (2011): 1494-1503.

Species:

Human

Sample Type:

Conditioned Media (release from PLGA engineered tissue)

View Publication

Nazarov I, Lee JW, Soupene E, et al. Multipotent Stromal Stem Cells from Human Placenta Demonstrate High Therapeutic Potential. Stem Cells Translational Medicine. 2012;1(5):359-372. doi:10.5966/sctm.2011-0021.

Species:

Human

Sample Type:

Conditioned Media ( human chorionic mesenchymal stem cell derived from placenta MSCs derived from human bone marrow)

View Publication

Guan P., Guo J., Yu X., et al. The role of cyclooxygenase-2, interleukin-1? and fibroblast growth factor-2 in the activation of matrix metalloproteinase-1 in sheared-chondrocytes and articular cartilage. Sci Rep. 2015 May 20;5:10412. doi: 10.1038/srep10412.

Species:

Human

Sample Type:

Conditioned Media (Human Chondrocytes)

View Publication

Kumar A, Jovel J, Lopez-Orozco J, Limonta D, Airo AM, Hou S, Stryapunina I, Fibke C, Moore RB, Hobman TC. Human Sertoli cells support high levels of Zika virus replication and persistence. Sci Rep. 2018 Apr 3;8(1):5477. doi: 10.1038/s41598-018-23899-x. PMID: 29615760; PMCID: PMC5883016.

Species:

Human

Sample Type:

Conditioned Media

View Publication

Brown K., Lau J., Dom A., et al. MG624, an ?7-nAChR antagonist, inhibits angiogenesis via the Egr-1/FGF2 pathway. Angiogenesis. 2012 Mar;15(1):99-114. doi: 10.1007/s10456-011-9246-9.

Species:

Human

Sample Type:

Conditioned Media (Human Microvascular Endothelial Cells of the Lugn Culture Sup)

View Publication

Wei L, Wu S, Kuss M, Jiang X, Sun R, Reid P, Qin X, Duan B. 3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering. Bioact Mater. 2019 Sep 25;4:256-260. doi: 10.1016/j.bioactmat.2019.09.001. PMID: 31667442; PMCID: PMC6812411.

Species:

Human

Sample Type:

Plasma (prp)

View Publication

Sowmya S., Chennazhi K., Arzate H., et al. Periodontal Specific Differentiation of Dental Follicle Stem Cells into Osteoblast, Fibroblast, and Cementoblast. Tissue Eng Part C Methods. 2015 Oct;21(10):1044-58. doi: 10.1089/ten.TEC.2014.0603.

Species:

Human

Sample Type:

Conditioned Media (Dental Follicle Stem Cells)

View Publication

Hironori Y., Yoshihisa H., Hisayuki K.

Species:

Human

Sample Type:

Conditioned Media (A549 and WA-hT cancer cell lines with THP-1 cells)

View Publication

View All Publications For This Product

Reviews

Write a customer review

Amazing ELISA kit

Rating
100%

I used the media which contained FGF2 release over a period of 7 days through controlled release using alginate microtubes injected with FGF2. Pretty detailed procedure and explanations to prepare solutions are easily understandable

Review by Dipayan,

from SUNY Polytechnic Institute, Albany,

on Jun 12, 2023
Great product

Rating
100%

Easy to use and allowed to run different conditions

Review by Carlos A. Barreto Gamarra,

from University of Puerto Rico,

on Sep 1, 2022
Great product

Rating
100%

Easy to follow protocol with good performance and reproducibility. Reagents must be stored and used following vendor recommendations better results.

Review by Said,

from University of Puerto Rico,

on Jul 14, 2022
Great product

Rating
100%

I used the cell culture supernatant, sometimes, you need to work on different dilution factor

Review by Samia,

from Florida A&M University,

on Dec 17, 2020
Great product

Rating
100%

Our lab utilized this ELISA kit to obtain release of bFGF from our biomaterial system over long periods. The detection range on this kit is great (50 pg/mL to 10000 pg/ml) for this application. The instruction manual is easy to follow. We chose this product because RayBiotech has always been a great company to us.

Review by J.Jameson,

from University of Florida,

on Feb 8, 2020
Great product

Rating
100%

The ELISA instructions are concise and easy to follow. The kit also has all reagents you need. It has great reproducibility and reliability.

Review by Julie Jameson,

from University of Florida,

on Sep 24, 2019

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